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A new phenotype of Polymyxa betae in Arabidopsis thaliana

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Abstract

The understanding of the molecular biology of Polymyxa betae, the protist vector of Beet necrotic yellow vein virus, remains limited because of the obligate nature of this root endoparasite and the limited data on the genome of Beta vulgaris, its most common host plant. The aim of this work was to assess the infection of P. betae in Arabidopsis thaliana in order to learn more about the P. betae genome and its interaction with the host. The susceptibility of a set of ecotypes of various origins to a monosporosorus and aviruliferous isolate of P. betae was analyzed in a series of bioassays conducted under controlled conditions. P. betae was detected in roots of A. thaliana using light microscopy and PCR. The infection severity was relatively low in this species compared with B. vulgaris, but the different stages of the life cycle were present. The phenotype of P. betae in A. thaliana root cells differed from the phenotype in B. vulgaris: the spore-forming phase was more prevalent in comparison with the sporangial phase, and the sporosori contained a lower number of spores. The compatible interaction between P. betae and A. thaliana obtained after the inoculation of zoospores and optimal conditions for the development of P. betae provide a new model system that can be used to improve the knowledge on the P. betae genome and on the mechanisms of the spore-forming phase of P. betae.

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Acknowledgements

We thank François Crutzen, Benjamin Dieryck, Yann Galein and Jeannine Weyns for their scientific advice and help. Nicolas Desoignies is a fellow of UCL Special Research Funds (FSR) and the Belgian FRIA fund.

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Authors and Affiliations

  1. Université catholique de Louvain, Earth and Life Institute, Phytopathology, Croix du sud 2 bte 3, 1348, Louvain-la-Neuve, Belgium

    Nicolas Desoignies, Celine Stocco, Claude Bragard & Anne Legrève

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  1. Nicolas Desoignies
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  2. Celine Stocco
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  3. Claude Bragard
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  4. Anne Legrève
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Correspondence to Anne Legrève.

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Desoignies, N., Stocco, C., Bragard, C. et al. A new phenotype of Polymyxa betae in Arabidopsis thaliana . Eur J Plant Pathol 131, 27–38 (2011). https://doi.org/10.1007/s10658-011-9783-5

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