Abstract
Rumple is a serious peel collapse of Primofiori lemons in the southeast of Spain with an unresolved aetiology. Symptoms typically occur on fruits at ripening under wet conditions as dark sunken lesions producing premature fruit drop and damaged fruits unacceptable for fresh commercialization. A total of 16 Colletotrichum spp. isolates established from rumple-affected lemons collected during the autumn of 2007 from two different orchards were characterized by molecular and phenotypic assays and compared with reference isolates. Species-specific PCR reactions using β-tubulin 2 nucleotide sequences showed Colletotrichum gloeosporioides to predominate (81.5%) with limited occurrence of C. acutatum (18.75%). Among the C. gloeosporioides isolates, five (38.5%) showed benomyl resistance and eight (61.5%) were highly sensitive to the fungicide. The limited occurrence of C. acutatum could be related to factors such as the presence of both species on the same fruit, unfavourable meteorological conditions and low disease incidence. This work reveals an association of C. gloeosporioides and C. acutatum isolates with rumple disease of lemons and expands the range of C. acutatum on citrus.
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Acknowledgements
This study was financed by the Programa de Desarrollo y Diversificación Económica de Zonas Rurales (PRODER) through the Grupo de Acción Local Vega Baja Desarrollo Rural (VEBADER), Expedient 7.4.060. The authors are grateful to Enrique Galbis Biosca from FITOGAL, S.L for the supply of fungicide.
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Valero, M., García-Martínez, S., Giner, M.J. et al. Benomyl sensitivity assays and species-specific PCR reactions highlight association of two Colletotrichum gloeosporioides types and C. acutatum with rumple disease on Primofiori lemons. Eur J Plant Pathol 127, 399–405 (2010). https://doi.org/10.1007/s10658-010-9606-0
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DOI: https://doi.org/10.1007/s10658-010-9606-0