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BAK1 and BKK1 in Arabidopsis thaliana confer reduced susceptibility to turnip crinkle virus

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Abstract

BAK1 and BKK1 are receptor-like protein kinases (RLKs) involved in brassinosteroid signal transduction and plant resistance to bacteria and fungi. Here we report that loss-of-function mutants of BAK1 or BKK1 in Col-0 showed enhanced susceptibility to Turnip crinkle virus (TCV) infection. Cell death and chlorosis occurred much earlier in bak1-4 and bkk1-1 mutants than in wild-type plants, suggesting an important role of BAK1 and BKK1 in controlling cell death associated with TCV infection. The bak1-4 and bkk1-1 mutants showed the elevated transcription levels of pathogenesis genes, such as PR1, PR2, PR5, PDF1.2. Enhanced expression of these genes, however, failed to resist the transport of TCV virus. At 9 days postinoculation, TCV levels were the highest in the systemic leaves of bak1-4, but were undetected in BAK1 or BKK1 overexpressing plants. These results signify that both BAK1 and BKK1 are important components in controlling TCV infection in Arabidopsis plants.

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Acknowledgments

This work was supported by the National Key Basic Research“973” Program of China (2009CB118500), National Natural Science Foundation of China (30970214, 30670166) and Project of Chinese Ministry of Education (108110). We thank Anne Simon for providing TCV antibody.

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Authors and Affiliations

  1. School of Life Sciences, Sichuan University, Chengdu, 610064, People’s Republic of China

    Hui Yang, Dehui Xi, Junbo Du & Honghui Lin

  2. Department of Botany and Microbiology, University of Oklahoma, Norman, OK, 73019, USA

    Hui Yang, Xiaoping Gou, Kai He & Jia Li

  3. School of Life Sciences, Lanzhou University, Lanzhou, 730000, People’s Republic of China

    Xiaoping Gou & Jia Li

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  1. Hui Yang
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  2. Xiaoping Gou
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  3. Kai He
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  4. Dehui Xi
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  5. Junbo Du
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  6. Honghui Lin
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  7. Jia Li
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Correspondence to Honghui Lin or Jia Li.

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Yang, H., Gou, X., He, K. et al. BAK1 and BKK1 in Arabidopsis thaliana confer reduced susceptibility to turnip crinkle virus . Eur J Plant Pathol 127, 149–156 (2010). https://doi.org/10.1007/s10658-010-9581-5

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