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First report of Monilia polystroma on apple in Hungary

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Abstract

A Monilinia fructigena-like isolate (UFT) was collected from apple shoots in northeastern Hungary (Újfehértó). Brownish dieback and buff-coloured stromata were observed on shoots and small fruits of cv. ‘Ashton Bitter’. On potato dextrose agar (PDA) the colonies were yellowish in colour and irregular black stromatal crusts occurred. Conidia (16.6 × 10.1 µm) were slightly smaller than the average of M. fructigena. The fungus caused brown rot on inoculated apple fruits, and produced numerous sporodochia. The sequences of the rDNA internal transcribed spacer regions of the UFT isolate were almost identical to that of a previously described Monilia polystroma isolate, containing all five nucleotides that distinguish it from M. fructigena. Comparison of a genomic sequence of unknown function revealed that repetitive sequence motifs occurred in different numbers as insertions in the genomes of M. fructigena, Monilia polystroma, and the UFT isolate. Classical and molecular characterisation indicated that the UFT isolate belonged to Monilia polystroma. To our knowledge this is the first report of Monilia polystroma in Europe.

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References

  • Batra, L. R. (1991). World species of Monilinia (Fungi): Their ecology, biosystematics and control. Mycologia Memoir No. 16. Berlin: Cramer.

    Google Scholar 

  • Bryde, R. J. W., & Willetts, H. J. (1977). The brown rot fungi of fruit. Their biology and control. Oxford: Pergamon.

    Google Scholar 

  • Côté, M. J., Tardif, M. C., & Meldrum, A. J. (2004). Identitification of Monilinia fructigena, M. fructicola, M. laxa, and Monilia polystroma on inoculated and naturally infected fruit using multiplex PCR. Plant Disease, 99, 1219–1225. doi:10.1094/PDIS.2004.88.11.1219.

    Article  Google Scholar 

  • Dellaporte, S. L., Wood, J., & Hicks, J. B. (1983). A plant DNA minipreparation: Version II. Plant Molecular Biology Reporter, 1, 19–23. doi:10.1007/BF02712670.

    Article  Google Scholar 

  • Fulton, C. E., & Brown, A. E. (1997). Use of SSU rDNA group-I intron to distinguish Monilinia fructicola from M. laxa and M. fructigena. FEMS Microbiology Letters, 157, 307–312.

    PubMed  CAS  Google Scholar 

  • Fulton, C. E., van Leeuwen, G. C. M., & Brown, A. E. (1999). Genetic variation among and within Monilinia species causing brown rot of stone and pome fruits. European Journal of Plant Pathology, 105, 495–500. doi:10.1023/A:1008711107347.

    Article  Google Scholar 

  • Gell, I., Cubero, J., & Melgarejo, P. (2007). Two different PCR approaches for universal diagnosis of brown rot and identification of Monilinia spp. in stone fruit trees. Journal of Applied Microbiology, 103, 2629–2637. doi:10.1111/j.1365-2672.2007.03495.x.

    Article  PubMed  CAS  Google Scholar 

  • Holb, I. J. (2004). Effect of acidity on growth rate and stroma formation of Monilia fructigena and M polystroma isolates. International Journal of Horticultural Science, 10, 63–67.

    Google Scholar 

  • Ioos, R., & Frey, P. (2000). Genomic variation within Monilinia laxa, M. fructigena and M. fructicola, and application to species identification by PCR. European Journal of Plant Pathology, 106, 373–378. doi:10.1023/A:1008798520882.

    Article  CAS  Google Scholar 

  • Maniatis, T., Sambrook, J., & Fritsch, E. F. (1989). Molecular cloning: A laboratory manual. New York: Cold Spring Harbor Laboratory.

    Google Scholar 

  • OEPP/EPPO. (2003). EPPO standards, diagnostic protocols for regulated pests. EPPO Bulletin, 33, 245–247. doi:10.1046/j.1365-2338.2003.00629.x.

    Article  Google Scholar 

  • Shima, Y. (1936). Studies on the young fruit rot of apple tree. Journal of the Faculty of Agriculture, Hokkaido University, 39, 143–149.

    Google Scholar 

  • van Leeuwen, G. C. M., Baayen, R. P., Holb, I. J., & Jeger, M. J. (2002). Distinction of the Asiatic brown rot fungus Monilia polystroma sp nov from Monilia fructigena. Mycological Research, 106, 444–451. doi:10.1017/S0953756202005695.

    Article  Google Scholar 

  • Willets, H. J., & Bullock, S. (1993). Cytology, histology and histochemistry of fruit infection by Monilinia species. In A. R. Biggs (Ed.), Handbook of cytology, histology and histochemistry of fruit tree diseases, pp. 113–136. Boca Raton: CRC.

    Google Scholar 

  • Wormald, H. (1954). The brown rot diseases of fruit trees. London: Her Majesty’s Stationery Office.

    Google Scholar 

Download references

Acknowledgements

The authors gratefully acknowledge the technical assistance of A. Szigethy. The work was supported by grants NKFP-A2-2006-0084, OM-00034/2007 (NKTH, Jedlik Ányos programme) and GVOP-3.2.1-2004-04- 0134/3.0.

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  1. Faculty of Horticultural Science, Department of Plant Pathology, Corvinus University of Budapest, 44 Ménesi Road, Budapest, H-1118, Hungary

    Marietta Petróczy & László Palkovics

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  1. Marietta Petróczy
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  2. László Palkovics
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Correspondence to László Palkovics.

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Petróczy, M., Palkovics, L. First report of Monilia polystroma on apple in Hungary. Eur J Plant Pathol 125, 343–347 (2009). https://doi.org/10.1007/s10658-009-9476-5

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