Abstract
Actinidia chinensis and A. deliciosa plants from China, showing a range of symptoms, including vein clearing, interveinal mottling, mosaics and chlorotic ring spots, were found to contain ~300 nm rod-shaped virus particles. The virus was mechanically transmitted to several herbaceous indicators causing systemic infections in Nicotiana benthamiana, N. clevelandii, and N. occidentalis, and local lesions in Chenopodium quinoa. Systemically- infected leaves reacted with a Tobacco mosaic virus polyclonal antibody in indirect ELISA. PCR using generic and specific Tobamovirus primers produced a 1,526 bp sequence spanning the coat protein (CP), movement protein (MP), and partial RNA replicase genes which showed a maximum nucleotide identity (88%) with Turnip vein clearing virus and Penstemon ringspot virus. However, when the CP sequence alone was considered the highest CP sequence identity (96% nt and 98% aa) was to Ribgrass mosaic virus strain Kons 1105. The morphological, transmission, serological and molecular properties indicate that the virus is a member of subgroup 3 of the genus Tobamovirus.
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Acknowledgements
The authors wish to thank Zespri Innovation and Hort Research for funding this work, Jane Lancaster and Peter Berry (Zespri Innovation) for their management of the quarantine plants and associated testing programme, and Liz Hardiman, Raja Thangavel and Anton Russell (University of Auckland) for technical assistance.
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Chavan, R.R., Pearson, M.N. & Cohen, D. Partial characterisation of a novel Tobamovirus infecting Actinidia chinensis and A. deliciosa (Actinidiaceae) from China. Eur J Plant Pathol 124, 247–259 (2009). https://doi.org/10.1007/s10658-008-9409-8
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DOI: https://doi.org/10.1007/s10658-008-9409-8