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Genetic variability of Plum pox virus isolates in the Czech Republic

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Abstract

Plum pox virus (PPV), the causal agent of Sharka disease, is an important pathogen of stone fruit trees. In this study, 24 new Czech PPV isolates from five different orchards were collected and characterized, molecularly. PPV-D isolates were identified in all orchards studied; whereas PPV-Rec isolates were identified in only two of them. A phylogenetic analysis on (Cter) NIb-(Nter) CP was performed. Three Czech PPV-D isolates BOH11CZ, BOH12CZ, and BOH13CZ diverged into a significantly separated cluster. PPV-Rec isolates formed a fairly homogenous group. However, the Bohutice and the Lipov PPV-Rec isolates clustered in two significantly separated branches.

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References

  • Candresse, T., Cambra, M., & Dallot, S. (1998). Comparison of monoclonal antibodies and polymerase chain reaction assays for the typing of isolates belonging to the M and D serotypes of plum pox potyvirus. Phytopathology, 88, 198–204.

    Article  CAS  PubMed  Google Scholar 

  • Candresse, T., Svanella-Dumas, L., Gentit, P., Caglayan, K., & Cevic, B. (2007). First report of the presence of Plum pox virus Rec strain in Turkey. Plant Disease, 91, 331–331.

    Article  Google Scholar 

  • Glasa, M., Palkovics, L., Komínek, P., Labonne, G., Pittnerová, S., Kudela, O., Candresse, T., & Šubr, Z. (2004). Geographically and temporally distant natural recombinant isolates of Plum pox virus (PPV) are genetically very similar and form a unique PPV subgroup. Journal of General Virology, 85, 2671–2681.

    Article  PubMed  CAS  Google Scholar 

  • Glasa, M., Paunovic, S., Jevremovic, D., Myrta, A., Pittnerova, S., & Candresse, T. (2005). Analysis of recombinant Plum pox virus (PPV) isolates from Serbia confirms genetic homogeneity and supports a regional origin for the PPV-Rec subgroup. Archives of Virology, 150, 2051–2060.

    Article  PubMed  CAS  Google Scholar 

  • Glasa, M., Veronique, M. J., Labonne, G., Šubr, Z., Kúdela, O., & Quiot, J. B. (2002). A natural population of recombinant Plum pox virus is viable and competitive under field conditions. European Journal of Plant Pathology, 108, 843–853.

    Article  CAS  Google Scholar 

  • Guindon, S., & Gascuel, O. (2003). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology, 52, 696–704.

    Article  PubMed  Google Scholar 

  • James, D., & Varga, A. (2005). Nucleotide sequence analysis of Plum pox virus isolate W3174: Evidence of a new strain. Virus Research, 110, 143–150.

    Article  PubMed  CAS  Google Scholar 

  • James, D., Varga, A., Thompson, D., & Hayes, S. (2003). Detection of new and unusual isolate of Plum pox virus in plum (Prunus domestica). Plant Disease, 87, 1119–1124.

    Article  CAS  Google Scholar 

  • Kerlan, C., & Dunez, J. (1979). Différenciation biologique et sérologique des souches du virus de la sharka. Annales de Phytophathologies, 11, 241–250.

    Google Scholar 

  • Kollerová, E., Novaková, S., Šubr, Z., & Glasa, M. (2006). Plum pox virus mixed infection detected in apricot in Pakistan. Plant Disease, 90, 1108.

    Article  Google Scholar 

  • Matić, S., Al-Rwahnih, M., & Myrta, A. (2006). Diversity of Plum pox virus isolates in Bosnia and Herzegovina. Plant Pathology, 55, 11–17.

    Article  CAS  Google Scholar 

  • Navrátil, M., Šimonová, V., Fialová, R., & Válová, P. (1998). Molecular variability of Czech Plum pox virus isolates. Acta Virologica, 42, 254–256.

    PubMed  Google Scholar 

  • Nemchinov, L., & Hadidi, A. (1996). Characterization of the sour cherry strain of Plum pox virus. Phytopathology, 86, 575–580.

    Article  Google Scholar 

  • Posada, D., & Crandall, K. A. (1998). Modeltest: testing the model of DNA substitution. Bioinformatics, 14, 817–818.

    Article  PubMed  CAS  Google Scholar 

  • Salamon, P., & Palkovics, L. (2002). Characterization of Plum pox virus PPV-BT-H isolated from naturally infected blackthorn (Prunus spinosa L.) in Hungary. European Journal of Plant Pathology, 108, 903–907.

    Article  CAS  Google Scholar 

  • Spiegel, S., Kovalenko, E. M., Varga, A., & James, D. (2004). Detection and partial molecular characterization of two Plum pox virus isolates from plum and wild apricot in Southern Kazakhstan. Plant Disease, 88, 973–979.

    Article  CAS  Google Scholar 

  • Šubr, Z., Pittnerová, S., & Glasa, M. (2004). A simplified RT-PCR-based detection of recombinant Plum pox virus isolates. Acta Virologica, 48, 173–176.

    PubMed  Google Scholar 

  • Swofford, D. L. (2002). PAUP*: Phylogenetic analysis using parsimony (* and other methods), version 4.0b10. Sunderland, MA: Sinauer.

    Google Scholar 

  • Varga, A., & James, D. (2005). Detection and differentiation of Plum pox virus using real-time multiplex PCR with SYBR Green and melting curve analysis: a rapid method for strain typing. Journal of Virological Methods, 123, 213–220.

    Article  PubMed  CAS  Google Scholar 

  • Wetzel, T., Candresse, T., Ravelonandro, M., Delbos, R. P., Mazyad, H., Aboul-Ata, A. E., et al. (1991). Nucleotide sequence of the 3′-terminal region of the RNA of the El Amar strain of Plum pox potyvirus. Journal of General Virology, 72, 1741–1746.

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

The work was supported by the Ministry of Education of the Czech Republic grant number MSM 6198959215. We thank Dr. T. Candresse for critical reading of the manuscript, Dr. L. Bocák and MSc. Z. Levkaničová for obvious discussion on phylogenetic analysis, and Peter Lemkin for manuscript editing.

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  1. Palacký University in Olomouc, Šlechtitelů 11, 783 71, Olomouc, Czech Republic

    Sébastien Gadiou, Dana Šafářová & Milan Navrátil

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  1. Sébastien Gadiou
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  2. Dana Šafářová
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  3. Milan Navrátil
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Correspondence to Sébastien Gadiou.

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Gadiou, S., Šafářová, D. & Navrátil, M. Genetic variability of Plum pox virus isolates in the Czech Republic. Eur J Plant Pathol 121, 513–517 (2008). https://doi.org/10.1007/s10658-008-9272-7

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  • DOI: https://doi.org/10.1007/s10658-008-9272-7

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