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Tobacco cultivars vary in induction of systemic resistance against Cucumber mosaic virus and growth promotion by Pseudomonas chlororaphis O6 and its gacS mutant

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Abstract

The colonization of plant roots with certain rhizosphere bacteria promotes plant growth and induces long lasting systemic protection against a broad spectrum of plant pathogens. The role of the global regulator, GacS, in the rhizosphere colonist Pseudomonas chlororaphis O6 in stimulating growth promotion and induced resistance against Cucumber mosaic virus was examined in tobacco. Responses were compared in tobacco cvs Samsun and GX3. Root colonization of Samsun with wild-type O6 and the gacS complemented mutant-elicited reduced viral symptoms and viral titre. On GX3, there was little affect on symptoms when roots were colonized by the wild-type, gacS mutant or complemented mutant but colonization by both the wild-type and the gacS mutant lowered viral titre. Wild-type O6 and the gacS mutant caused plant growth to be maintained in both tobacco cultivars after viral infection, although the affect was stronger with GX3 than Samsun. In contrast, although a chemical inducer, benzothiadiazole, reduced symptoms and viral titre in both cultivars, plant growth was suppressed. Our results indicate rhizobacteria-elicited induced viral resistance without a negative impact on growth but there was a differential response between cultivars. Detailed knowledge regarding the mechanisms inherent to these differences between cultivars requires further investigation.

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Acknowledgements

This work was supported by a grant from BioGreen 21 programme (code#20050401034716), Rural Development Administration, Republic of Korea, by a grant from the Technology Development Programme for Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea, and by the Utah State Agricultural Experiment Station, USA.

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Authors and Affiliations

  1. Laboratory of Microbial Genomics, Systems Microbiology Research Center, KRIBB, Daejeon, 305-600, South Korea

    Choong-Min Ryu

  2. Environmental-Friendly Agriculture Research Center, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, 500-757, South Korea

    Beom Ryong Kang, Song Hee Han, Song Mi Cho & Young Cheol Kim

  3. Department of Entomology and Plant Pathology, Auburn University, Auburn, AL, 36849, USA

    Choong-Min Ryu & Joseph W. Kloepper

  4. Department of Biology, Utah State University, Logan, UT, 84322-5305, USA

    Anne J. Anderson

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  1. Choong-Min Ryu
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  2. Beom Ryong Kang
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  3. Song Hee Han
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  4. Song Mi Cho
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  5. Joseph W. Kloepper
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  6. Anne J. Anderson
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  7. Young Cheol Kim
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Correspondence to Young Cheol Kim.

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Ryu, CM., Kang, B.R., Han, S.H. et al. Tobacco cultivars vary in induction of systemic resistance against Cucumber mosaic virus and growth promotion by Pseudomonas chlororaphis O6 and its gacS mutant. Eur J Plant Pathol 119, 383–390 (2007). https://doi.org/10.1007/s10658-007-9168-y

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  • DOI: https://doi.org/10.1007/s10658-007-9168-y

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