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Expressed sequence tags from the phytopathogenic fungus Botrytis cinerea

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Abstract

A large set of genes was identified in the phytopathogenic fungus Botrytis cinerea by using an expressed sequence tag approach. The fungus was grown in axenic culture and a cDNA library was produced. From this library, 6559 ESTs were obtained. The combined sequences represent 3026 unisequences that corresponds to approximately one-quarter of the estimated total number of genes in B. cinerea. Approximately 18% of the ESTs showed significant similarities with genes coding for proteins with known functions,~56% were similar to genes coding for proteins with unknown functions and ~26% were orphans. A substantial B. cinerea gene inventory including putative virulence factors was therefore obtained and is now available at the Génoplante-Info Database interface (http://urgi.infobiogen.fr///Projects/GPiDB/Interface/).

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Abbreviations

CWDEs:

cell wall degrading enzymes

ESTs:

expressed sequence tags

GPiDB:

Génoplante-Info DataBase.

References

  • SF Altschul TL Madden AA Schaffer Z Zhang W Miller DJ Lipman (1997) ArticleTitleGapped BLAST and PSI-BLAST: A new generation of protein database programs Nucleic Acids Research 25 3389–3402 Occurrence Handle9254694 Occurrence Handle1:CAS:528:DyaK2sXlvFyhu7w%3D

    PubMed  CAS  Google Scholar 

  • AM Calvo RA Wilson JW Bok NP Keller (2002) ArticleTitleRelationship between secondary metabolism and fungal development Microbiology and Molecular Biology Reviews 66 447–459 Occurrence Handle12208999 Occurrence Handle1:CAS:528:DC%2BD38XnsFOitro%3D

    PubMed  CAS  Google Scholar 

  • Z Caracuel MI Roncero EA Espeso CI Gonzalez-Verdejo FI Garcia-Maceira Pietro A Di (2003) ArticleTitleThe pH signalling transcription factor PacC controls virulence in the plant pathogen Fusarium oxysporum Molecular Microbiology 48 765–779 Occurrence Handle12694620 Occurrence Handle1:CAS:528:DC%2BD3sXjsFKqt7o%3D

    PubMed  CAS  Google Scholar 

  • MJ de Carvalho RS Amorim Jesuino BS Daher I Silva-Pereira SM de Freitas CM Soares MS Felipe (2003) ArticleTitleFunctional and genetic characterization of calmodulin from the dimorphic and pathogenic fungus Paracoccidioides brasiliensis Fungal Genetics and Biology 39 204–210 Occurrence Handle12892633 Occurrence Handle1:CAS:528:DC%2BD3sXlvVCru7w%3D

    PubMed  CAS  Google Scholar 

  • AE Desjardins TM Hohn (1997) ArticleTitleMycotoxins in plant pathogenesis Molecular Plant-Microbe Interactions 10 147–152 Occurrence Handle1:CAS:528:DyaK2sXhsFKiurg%3D

    CAS  Google Scholar 

  • Y Elad K Evenson (1995) ArticleTitlePhysiological aspects of resistance to Botrytis cinerea Phytopathology 85 637–643

    Google Scholar 

  • DS Fox J Heitman (2002) ArticleTitleGood fungi gone bad: the corruption of calcineurin BioEssays 24 894–903 Occurrence Handle12325122 Occurrence Handle1:CAS:528:DC%2BD38Xot1ajsb4%3D

    PubMed  CAS  Google Scholar 

  • FM Freimoser S Screen S Bagga G Hu RJ St Leger (2003) ArticleTitleExpressed sequence tag (EST) analysis of two subspecies of Metarhizium anisopliae reveals a plethora of secreted proteins with potential activity in insect hosts Microbiology 149 239–247 Occurrence Handle12576597 Occurrence Handle1:CAS:528:DC%2BD3sXnvV2ltg%3D%3D

    PubMed  CAS  Google Scholar 

  • JE Galagan C Nusbaum A Roy MG Endrizzi P Macdonald W FitzHugh S Calvo R Engels S Smirnov D Atnoor A Brown N Allen J Naylor N Stange-Thomann K DeArellano R Johnson L Linton P McEwan K McKernan J Talamas A Tirrell W Ye A Zimmer RD Barber I Cann DE Graham DA Grahame AM Guss R Hedderich C Ingram-Smith HC Kuettner JA Krzycki JA Leigh W Li J Liu B Mukhopadhyay JN Reeve K Smith TA Springer LA Umayam O White RH White E de Macario Conway JG Ferry KF Jarrell H Jing AJ Macario I Paulsen M Pritchett KR Sowers RV Swanson SH Zinder E Lander WW Metcalf B Birren (2003) ArticleTitleThe genome sequence of the filamentous fungus Neurospora crassa Nature 422 859–868 Occurrence Handle10.1038/nature01554 Occurrence Handle1:CAS:528:DC%2BD3sXjtFCitb4%3D Occurrence Handle12712197

    Article  CAS  PubMed  Google Scholar 

  • E Glémet JJ Codani (1997) ArticleTitleLASSAP, a Large Scale Sequence compArison Package Computer Applications in the Biosciences 13 137–143 Occurrence Handle9146960

    PubMed  Google Scholar 

  • M Gourgues P-H Clergeot C Veneault J Cots S Sibuet A Brunet-Simon C Levis T Langin M-H Lebrun (2002) ArticleTitleA new class of tetraspanins in fungi Biochemical and Biophysical Research Communications 297 1197–1204 Occurrence Handle12372414 Occurrence Handle1:CAS:528:DC%2BD38XnsFSnsLs%3D

    PubMed  CAS  Google Scholar 

  • M Gourgues A Brunet-Simon M-H Lebrun C Levis (2004) ArticleTitleThe tetraspanin BcPls1p is required for appressorium-mediated penetration of Botrytis cinerea into host plant leaves Molecular Microbiology 51 619–629 Occurrence Handle14731267 Occurrence Handle1:CAS:528:DC%2BD2cXhtlWitL8%3D

    PubMed  CAS  Google Scholar 

  • S Higuchi J Tamura PR Giri JW Polli RL Kincaid (1999) ArticleTitleCalmodulin-dependent protein phosphatase from Neurospora crassa Molecular cloning and expression of recombinant catalytic subunit. Journal of Biological Chemistry 266 18104–18112

    Google Scholar 

  • TM Hohn AE Desjardins SP McCormick (1995) ArticleTitleThe Tri4 gene of Fusarium sporotrichioides encodes a cytochrome P450 monooxygenase involved in trichothecene biosynthesis Molecular and General Genetics 248 95–102 Occurrence Handle7651333 Occurrence Handle1:CAS:528:DyaK2MXnvFKmtr8%3D

    PubMed  CAS  Google Scholar 

  • X Huang A Madan (1999) ArticleTitleCAP3: A DNA sequence assembly program Genome Research 9 868–877 Occurrence Handle10508846 Occurrence Handle1:CAS:528:DyaK1MXmslKgs7Y%3D

    PubMed  CAS  Google Scholar 

  • CS Hwang MA Flaishman PE Kolattukudy (1995) ArticleTitleCloning of a gene expressed during appressorium formation by Colletotrichum gloeosporioides and a marked decrease in virulence by disruption of this gene Plant Cell 7 183–193 Occurrence Handle7756829 Occurrence Handle1:CAS:528:DyaK2MXktVOktrk%3D

    PubMed  CAS  Google Scholar 

  • J Jurka (2000) ArticleTitleRepbase Update: A database and an electronic journal of repetitive elements Trends in Genetics 16 418–420 Occurrence Handle10973072 Occurrence Handle1:CAS:528:DC%2BD3cXmvFygtr0%3D

    PubMed  CAS  Google Scholar 

  • A Justesen S Somerville S Christiansen H Giese (1996) ArticleTitleIsolation and characterization of two novel genes expressed in germinating conidia of the obligate biotrophe Erysiphe graminis Gene 170 131–135 Occurrence Handle8621073 Occurrence Handle1:CAS:528:DyaK28XitFChsr4%3D

    PubMed  CAS  Google Scholar 

  • S Kamoun P Hraber B Sobral D Nuss F Govers (1999) ArticleTitleInitial assessment of gene diversity for the oomycete pathogen Phytophthora infestans based on expressed sequences Fungal Genetics and Biology 28 94–106 Occurrence Handle10587472 Occurrence Handle1:CAS:528:DyaK1MXnslWrur8%3D

    PubMed  CAS  Google Scholar 

  • J Keon A Bailey J Hargreaves (2000) ArticleTitleA group of expressed cDNA sequences from the wheat fungal leaf blotch pathogen Mycosphaerella graminicola (Septoria tritici) Fungal Genetics and Biology 29 118–133 Occurrence Handle10919380 Occurrence Handle1:CAS:528:DC%2BD3cXltFShsb0%3D

    PubMed  CAS  Google Scholar 

  • YK Kim D Li PE Kolattukudy (1998) ArticleTitleInduction of Ca2+calmodulin signaling by hard-surface contact primes Colletotrichum gloeosporioides conidia to germinate and form appressoria Journal of Bacteriology 180 5144–5150 Occurrence Handle9748448 Occurrence Handle1:CAS:528:DyaK1cXms1ehs7k%3D

    PubMed  CAS  Google Scholar 

  • S Kim IP Ahn YH Lee (2001) ArticleTitleAnalysis of genes expressed during rice-Magnaporthe grisea interactions Molecular Plant-Microbe Interactions 14 1340–1346 Occurrence Handle11763134 Occurrence Handle1:CAS:528:DC%2BD3MXotFGjtLk%3D

    PubMed  CAS  Google Scholar 

  • A Klimpel C Schulze Gronover B Williamson JA Stewart B Tudzinsky (2002) ArticleTitleThe adenylate cyclase (BAC) in Botrytis cinerea is required for full pathogenicity Molecular Plant-Microbe Interactions 3 439–450 Occurrence Handle1:CAS:528:DC%2BD38Xps1Knsrk%3D

    CAS  Google Scholar 

  • S Kroken NL Glass JW Taylor OC Yoder BG Turgeon (2003) ArticleTitlePhylogenomic analysis of type I polyketide synthase genes in pathogenic and saprobic ascomycetes Proceedings of the National Academy of Sciences USA 100 15670–15675 Occurrence Handle1:CAS:528:DC%2BD2cXhtVCgtw%3D%3D

    CAS  Google Scholar 

  • GO Kothe SJ Free (1998) ArticleTitleCalcineurin subunit B is required for normal vegetative growth in Neurospora crassa Fungal Genetics and Biology 23 248–258 Occurrence Handle9680955 Occurrence Handle1:CAS:528:DyaK1cXltl2hsrg%3D

    PubMed  CAS  Google Scholar 

  • J Kronstad A De Maria D Funnell RD Laidlaw N Lee MM de Sà Marilee Ramesh (1998) ArticleTitleSignaling via cAMP in fungi: Interconnections with mitogen-activated protein kinase pathways Archives of Microbiology 170 395–404 Occurrence Handle9799282 Occurrence Handle1:CAS:528:DyaK1cXnvFOmur4%3D

    PubMed  CAS  Google Scholar 

  • SC Lee YH Lee (1998) ArticleTitleCalcium/calmodulin-dependent signalling for appressorium formation in the plant pathogenic fungus Magnaporthe grisea Molecules and Cells 8 698–704 Occurrence Handle9895122 Occurrence Handle1:CAS:528:DyaK1MXjvVOruw%3D%3D

    PubMed  CAS  Google Scholar 

  • P Leroux R Fritz D Debieu C Albertini C Lanen J Bach M Gredt F Chapeland (2002) ArticleTitleMechanisms of resistance to fungicides in field strains of Botrytis cinerea Pesticide Management Sciences 58 876–888 Occurrence Handle1:CAS:528:DC%2BD38XmvFGjs74%3D

    CAS  Google Scholar 

  • C Levis M Dutertre D Fortini Y Brygoo (1997) ArticleTitleTransformation of Botrytis cinerea with the structural nitrate reductase gene (niaD) shows a high frequency of homologous recombination Current Genetics 32 157–162 Occurrence Handle9294265 Occurrence Handle1:CAS:528:DyaK2sXlvFCnurk%3D

    PubMed  CAS  Google Scholar 

  • MC Lorenz (2002) ArticleTitleGenomics approaches to fungal pathogenicity Current Opinion in Microbiology 5 378–378

    Google Scholar 

  • MJ Neumann KF Dobinson (2003) ArticleTitleSequence tag analysis of gene expression during pathogenic growth and microsclerotia development in the vascular wilt pathogen Verticillium dahliae Fungal Genetics and Biology 38 54–62 Occurrence Handle12553936 Occurrence Handle1:CAS:528:DC%2BD3sXmt1Wgug%3D%3D

    PubMed  CAS  Google Scholar 

  • B Poinssot E Vandelle M Bentéjac M Adrian C Levis Y Brygoo J Garin F Sicilia P Coutos-Thevenot A Pugin (2003) ArticleTitleThe endopolygalacturonase 1 from Botrytis cinerea activates grapevine defence reactions unrelated to its enzymatic activity Molecular Plant-Microbe Interactions 16 553–564 Occurrence Handle12795381 Occurrence Handle1:CAS:528:DC%2BD3sXjvFOqsrk%3D

    PubMed  CAS  Google Scholar 

  • H Prokisch O Yarden M Dieminger M Tropschug IB Barthelmess (1997) ArticleTitleImpairment of calcineurin function in Neurospora crassa reveals its essential role in hyphal growth, morphology and maintenance of Ca2+ gradient Molecular and General Genetics 256 104–114 Occurrence Handle9349701 Occurrence Handle1:CAS:528:DyaK2sXntVWjsLk%3D

    PubMed  CAS  Google Scholar 

  • D Prusky N Yakoby (2003) ArticleTitlePathogenic fungi: Leading or led by ambient pH Molecular Plant Pathology 4 509–516 Occurrence Handle1:CAS:528:DC%2BD3sXpvVygsrY%3D

    CAS  Google Scholar 

  • C Rasmussen C Garen S Brining RL Kincaid RL Means AR Means (1994) ArticleTitleThe calmodulin-dependent protein phosphatase catalytic sub-unit (calcineurin A) is an essential gene in Aspergillus nidulans European Molecular Biology Organization Journal 13 2545–2552 Occurrence Handle1:CAS:528:DyaK2cXlvFOhs7o%3D

    CAS  Google Scholar 

  • P Rauyaree W Choi E Fang B Blackmon RA Dean (2001) ArticleTitleGenes expressed during early stages of rice infection with the rice blast fungus Magnaporthe grisea Molecular Plant Pathology 2 347–355 Occurrence Handle1:CAS:528:DC%2BD38XjvFGntr8%3D

    CAS  Google Scholar 

  • JA Rollins MB Dickman (2001) ArticleTitlepH signaling in Sclerotinia sclerotiorum: Identification of a pacC/RIM1 homolog Applied and Environmental Microbiology 67 75–81 Occurrence Handle11133430 Occurrence Handle1:CAS:528:DC%2BD3MXjtVWhsA%3D%3D

    PubMed  CAS  Google Scholar 

  • J Sambrook EF Fritsch T Maniatis (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Cold Spring Harbor Laboratory Press NY, USA

    Google Scholar 

  • D Samson F Legeai E Karsenty S Reboux JB Veyrieras J Just E Barillot (2003) ArticleTitleGenoPlante-Info (GPI): A collection of databases and bioinformatics resources for plant genomics Nucleic Acids Research 31 179–182 Occurrence Handle12519976 Occurrence Handle1:CAS:528:DC%2BD3sXhvFSntL4%3D

    PubMed  CAS  Google Scholar 

  • HJ Schoonbeek JM Raaijmakers MA De Waard (2002) ArticleTitleFungal ABC transporters and microbial interactions in natural environments Molecular Plant-Microbe Interactions 15 1165–1172 Occurrence Handle12423022 Occurrence Handle1:CAS:528:DC%2BD38XosVGqsr4%3D

    PubMed  CAS  Google Scholar 

  • C Schulze Gronover D Kasulke P Tudzynski B Tudzynski (2001) ArticleTitleThe role of G protein alpha subunits in the infection process of the grey mold Botrytis cinerea Molecular Plant-Microbe Interactions 14 1293–1302

    Google Scholar 

  • W Skinner J Keon J Hargreaves (2001) ArticleTitleGene information for fungal plant pathogens from expressed sequences Current Opinion in Microbiology 4 381–386 Occurrence Handle11495798 Occurrence Handle1:CAS:528:DC%2BD3MXlslGlur4%3D

    PubMed  CAS  Google Scholar 

  • DM Soanes W Skinner J Keon J Hargreaves NJ Talbot (2002) ArticleTitleGenomics of phytopathogenic fungi and the development of bioinformatic resources Molecular Plant-Microbe Interactions 15 421–427 Occurrence Handle12036272 Occurrence Handle1:CAS:528:DC%2BD38XjslSit70%3D

    PubMed  CAS  Google Scholar 

  • JA Sweigard DJ Ebbole (2001) ArticleTitleFunctional analysis of pathogenicity genes in a genomics world Current Opinion in Microbiology 4 387–392 Occurrence Handle11495799 Occurrence Handle1:CAS:528:DC%2BD3MXlslGlur8%3D

    PubMed  CAS  Google Scholar 

  • A ten Have W Mulder J Visser Kan JAL van (1998) ArticleTitleThe endopolygalacturonase gene Bcpg1 is required for full virulence of Botrytis cinerea Molecular Plant-Microbe Interactions 11 1009–1016 Occurrence Handle9768518 Occurrence Handle1:CAS:528:DyaK1cXmtlCqsLs%3D

    PubMed  CAS  Google Scholar 

  • A ten Have W Oude Breuil JP Wubben J Visser JAL van Kan (2001) Botrytis cinerea endopolygalacturonase genes are differentially expressed in various plant tissues Fungal Genetics and Biology 33 97–105 Occurrence Handle1:CAS:528:DC%2BD3MXltFGlt7s%3D

    CAS  Google Scholar 

  • A ten Have KB Tenberge JAE Benen P Tudzynski J Visser JAL van Kan (2002) The contribution of cell wall degrading enzymes to pathogenesis of fungal pathogens Kempken (Eds) The Mycota XIAgricultural applications Springer-Verlag Berlin, Heidelberg, Germany 341–358

    Google Scholar 

  • SW Thomas SW Rasmussen MA Glaring JA Rouster SK Christiansen RP Oliver (2001) ArticleTitleGene identification in the obligate fungal pathogen Blumeria graminis by expressed sequence tag analysis Fungal Genetics and Biology 33 195–211 Occurrence Handle11495576 Occurrence Handle1:CAS:528:DC%2BD3MXlslyjsL0%3D

    PubMed  CAS  Google Scholar 

  • F Trail J-R Xu P San Miguel RG Halgren HC Kistler (2003) ArticleTitleAnalysis of expressed sequence tags from Giberella zeae (anamorph Fusarium graminearum) Fungal Genetics and Biology 38 187–197 Occurrence Handle12620255

    PubMed  Google Scholar 

  • A Tunlid NJ Talbot (2002) ArticleTitleGenomics of parasitic and symbiotic fungi Current Opinion in Microbiology 5 513–519 Occurrence Handle12354560 Occurrence Handle1:CAS:528:DC%2BD38Xnt1Ggu70%3D

    PubMed  CAS  Google Scholar 

  • O Valette-Collet A Cimerman P Reignault C Levis M Boccara (2003) ArticleTitleDisruption of Botrytis cinerea pectin methylesterase gene bcpme1 reduces virulence on several host plants Molecular Plant-Microbe Interactions 16 360–367 Occurrence Handle12744465 Occurrence Handle1:CAS:528:DC%2BD3sXisVehu7k%3D

    PubMed  CAS  Google Scholar 

  • JAL van Kan A Goverse CJB van der Vlugt-Bergmans (1993) ArticleTitleElectrophoretic karyotype analysis of Botrytis cinerea Netherlands Journal of Plant Pathology 99 119–128 Occurrence Handle10.1007/BF03041402 Occurrence Handle1:CAS:528:DyaK2cXmtVSlu7c%3D

    Article  CAS  Google Scholar 

  • T Vermeulen H Schoonbeek MA de Waard (2001) ArticleTitleThe ABC transporter BcatrB from Botrytis cinerea is a determinant of the activity of the phenylpyrrole fungicide fludioxonil Pesticide Management Sciences 57 393–402 Occurrence Handle1:CAS:528:DC%2BD3MXjsVektLY%3D

    CAS  Google Scholar 

  • MC Viaud PV Balhadere NJ Talbot (2002) ArticleTitleA Magnaporthe grisea cyclophilin acts as a virulence determinant during plant infection Plant Cell 14 917–930 Occurrence Handle11971145 Occurrence Handle1:CAS:528:DC%2BD38XjsFWktrg%3D

    PubMed  CAS  Google Scholar 

  • M Viaud A Brunet-Simon Y Brygoo J-M Pradier C Levis (2003) ArticleTitleIdentification of calcineurin and cyclophilin A-dependant genes by cDNA arrays analysis in the phytopathogenic fungus Botrytis cinerea Molecular Microbiology 50 1451–1465 Occurrence Handle14651630 Occurrence Handle1:CAS:528:DC%2BD3sXhtVShtbzJ

    PubMed  CAS  Google Scholar 

  • JD Walton (1996) ArticleTitleHost-selective toxins: Agents of compatibility Plant Cell 8 2139–2150

    Google Scholar 

  • JD Walton (2000) ArticleTitleHorizontal gene transfer and the evolution of secondary metabolite gene clusters in fungi: an hypothesis Fungal Genetics and Biology 30 167–171 Occurrence Handle11035938 Occurrence Handle1:CAS:528:DC%2BD3cXntlGhsrk%3D

    PubMed  CAS  Google Scholar 

  • J-R Xu (2000) ArticleTitleMAP kinases in fungal pathogens Fungal Genetics and Biology 31 137–152 Occurrence Handle11273677 Occurrence Handle1:CAS:528:DC%2BD3MXitFylt7o%3D

    PubMed  CAS  Google Scholar 

  • C Xue G Park W Choi L Zheng RA Dean J-R Xu (2002) ArticleTitleTwo novel fungal virulence genes specifically expressed in appressoria of the rice blast fungus Plant Cell 14 2107–2119 Occurrence Handle12215509 Occurrence Handle1:CAS:528:DC%2BD38XnsFemtbk%3D

    PubMed  CAS  Google Scholar 

  • G Yang MS Rose BG Turgeon OC Yoder (1996) ArticleTitleA polyketide synthase is required for fungal virulence and the production of the polyketide T-toxin Plant Cell 8 2139–2150 Occurrence Handle8953776 Occurrence Handle1:CAS:528:DyaK28XntlShs7g%3D

    PubMed  CAS  Google Scholar 

  • OC Yoder BG Turgeon (2001) ArticleTitleFungal genomics and pathogenicity Current Opinion in Plant Biology 4 315–321 Occurrence Handle11418341 Occurrence Handle1:CAS:528:DC%2BD3MXksVyktL8%3D

    PubMed  CAS  Google Scholar 

  • L Zheng M Campbell J Murphy S Lam J-R Xu (2000) ArticleTitleThe BMP1 gene is essential for pathogenicity in the gray mold fungus Botrytis cinerea Molecular Plant-Microbe Interactions 13 724–732 Occurrence Handle10875333 Occurrence Handle1:CAS:528:DC%2BD3cXkt1ahtLg%3D

    PubMed  CAS  Google Scholar 

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Viaud, M., Legeai, F., Pradier, JM. et al. Expressed sequence tags from the phytopathogenic fungus Botrytis cinerea . Eur J Plant Pathol 111, 139–146 (2005). https://doi.org/10.1007/s10658-004-1429-4

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