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The distribution characteristics of β-propeller phytase genes in rhizosphere sediment provide insight into species specialty from phytic mineralization in subtropical and tropical seagrass ecosystems

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Abstract

Seagrass meadows have seriously deteriorated in recent years. Seagrass associated phytate-mineralizing rhizobacteria potentially have functions related to seagrass nutrition, health and sustainable growth. The β-propeller phytases (BPPs) are the only phytase family in aquatic environments, but there are few studies on the BPP community structure of seagrass. In this study, clone libraries and quantitative PCR (qPCR) assays were used to compare the diversity and abundances of the BPP communities of Halodule endl, Halophila ovalis and Thalassia hemprichii in Xisha and Sanya, and to investigate the distribution characteristics of BPP genes in the rhizosphere sediment, which provedes insight into species specialty from phytic mineralization in subtropical and tropical seagrass ecosystems. The highest diversity of BPP genes was found for Thalassia hemprichii in Sanya Bay. Thalassia hemprichii in Sanya had higher abundances of BPPs, which were linked to Gammaproteobacteria. The BPP community diversity and OTUs of Thalassia hemprichii in Sanya were much higher than those of Thalassia hemprichii on Yongxing Island and Stone Island. The seagrass BPP communities had higher diversity and evenness from sampling sites with more human activity. The qPCR results showed that the abundance of phytate acid-degradating bacteria was approximately three times larger in Thalassia hemprichii rhizosphere sediment samples than in Halodule endl and Halophila ovalis rhizosphere sediment samples. This study highlighted that the diversity and abundances of bacteria genetically encoding BPP in the rhizosphere of Thalassia hemprichii were clearly higher than those of Halodule endl and Halophila ovalis. Further study of microbial phosphorus cycling will provide new insights into seagrass meadow ecosystems.

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Acknowledgements

The research was supported by Strategic Priority Research Program of the Chinese Academy of Sciences (XDA13020300), Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) (GML2019ZD0402), National Key Research and Development Program of China (2017YFC0506301, 2018YFC1406501, 2018FY100105), the National Natural Science Foundation of China (41676163, 41406191, 41676107, 41276114), Guangdong Province Public Welfare Research and Capacity Building Project (2015A020216016), Science and Technology Planning Project of Guangdong Province, China (2017B030314052), and Pearl River S&T Nova Program of Guangzhou (201806010017), Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences (ISEE2018ZD02), Guangdong Provincial Department of Education, China (2014KQNCX195). We thank all of the members of the tropical Marine Biological Research station and Research Station in Hainan and Daya Bay and Xisha/Nansha Ocean Observation for their work in the field sample collection.

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Authors and Affiliations

  1. CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 510301, Guangzhou, China

    Liyun Lin, Juan Ling, Qiuying Peng, Xiancheng Lin, Weiguo Zhou, Qingsong Yang, Manzoor Ahamad, Ying Zhang, Cong Wang, Youshao Wang & Junde Dong

  2. Southern Marine Science and Engineering Guangdong Laboratory, 511458, Guangzhou, China

    Liyun Lin, Juan Ling, Qiuying Peng, Xiancheng Lin, Weiguo Zhou, Qingsong Yang, Manzoor Ahamad, Ying Zhang, Cong Wang, Youshao Wang & Junde Dong

  3. University of Chinese Academy of Sciences, 100049, Beijing, China

    Liyun Lin, Qiuying Peng, Xiancheng Lin, Manzoor Ahamad, Ying Zhang, Cong Wang & Junde Dong

  4. School of Food Engineering and Biotechnology, Hanshan Normal University, 521041, Chaozhou, China

    Liyun Lin

  5. Tropical Marine Biological Research station in Hainan, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 572000, Sanya, China

    Juan Ling, Weiguo Zhou, Qingsong Yang, Youshao Wang & Junde Dong

  6. Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Science, 510301, Guangzhou, China

    Juan Ling, Qiuying Peng, Xiancheng Lin, Qingsong Yang, Ying Zhang & Junde Dong

  7. Ocean School, Yantai University, 264005, Yantai, China

    Yanying Zhang

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  1. Liyun Lin
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  2. Juan Ling
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Correspondence to Juan Ling or Junde Dong.

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Lin, L., Ling, J., Peng, Q. et al. The distribution characteristics of β-propeller phytase genes in rhizosphere sediment provide insight into species specialty from phytic mineralization in subtropical and tropical seagrass ecosystems. Ecotoxicology 30, 1781–1788 (2021). https://doi.org/10.1007/s10646-021-02425-2

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