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Proline metabolism and molecular cloning of AmP5CS in the mangrove Avicennia marina under heat stress

  • S.I. : Marine Costal Ecosystem and Ecotoxicology
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Abstract

Proline is one of the most important compatible osmolyte in cells, which accumulates in response to various stresses, including salt, water deficit, heavy metal, pathogen infection and extreme temperature. In this study, a growth chamber was employed to simulate heat environment for Avicennia marina seedlings. We detected some physiological indices in the leaves of A. marina at 40 °C, including the activity of delta-1-pyrroline-5-carboxylate synthase (P5CS), the content of free proline and soluble protein, transpiration rate and membrane permeability, and discussed the relationship between these five indices and heat resistant ability. And then a P5CS gene was cloned from A. marina using homologous cloning and rapid amplification of cDNA ends methods. It was designated as AmP5CS, encoding a protein that contained a feedback inhibition site of proline, proA, proB, conserved Leu zipper, GSA-DH domain and other functional domains of P5CS protein in high plants. Expression analysis of AmP5CS gene indicated it was involved in heat stress response. It is the first time that P5CS from A. marina has been cloned and the findings laid the foundation of figuring out heat resistant mechanisms and relieving heat damage, which is significant during global warming.

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Acknowledgements

This research was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDA23050200 and No. XDA13010500), the National Natural Science Foundation of China (No.U1901211, No. 41430966 and No. 41876126), the National Key Research and Development Plan (No. 2017FY100700) and the International Partnership Program of Chinese Academy of Sciences (No. 133244KYSB20180012).

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  1. State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China

    Jin Liu & You-Shao Wang

  2. Daya Bay Marine Biology Research Station, Chinese Academy of Sciences, Shenzhen, 518121, China

    Jin Liu & You-Shao Wang

  3. Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou, 510301, China

    You-Shao Wang

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Liu, J., Wang, YS. Proline metabolism and molecular cloning of AmP5CS in the mangrove Avicennia marina under heat stress. Ecotoxicology 29, 698–706 (2020). https://doi.org/10.1007/s10646-020-02198-0

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