Abstract
Silkworm (Bombyx mori) is an economic insect of the Lepidoptera. Chlorantraniliprole (CAP) exposure results in reduced growth and development of B. mori and failure in cocooning, seriously affecting the development of sericulture. To study the mechanisms underlying the damage to silkworm caused by sublethal doses of CAP, we examined the oxidative damage, the activities of digestive enzymes in midgut, and the expressions of midgut-related genes at the mRNA level. We found that CAP exposure inhibited the growth of silkworm, decreased the body mass and caused the accumulation of reactive oxygen species (ROS) [the levels of O2−, H2O2 and lipid peroxidation (MDA) were increased by 1.62-, 1.87- and 1.46-fold, respectively]. Moreover, we also found that the midgut cells were disintegrated, microvilli disappeared, the stroma became thinner, and the chromatin of nucleus became aggregated after CAP exposure by the analysis of transmission electron microscopy (TEM). In addition, the activities of digestive enzymes were dysregulated in midgut (the activities of α-amylase and trypsin were decreased 0.69- and 0.20-fold, respectively). Furthermore, digital gene expression (DGE) profiling analysis revealed that the expressions of oxidative phosphorylation pathway and antioxidant defense system related genes in midgut were decreased, indicating that it was the oxidative damage in midgut caused by CAP that mainly affected the growth of silkworm, rather than the toxicological effects of CAP. Collectively, this study provided valuable insights into the toxic effects of CAP on insects.
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Acknowledgements
This study was supported by the China Agriculture Research System (CARS-18-ZJ0106), the Science and Technology support Program of Huzhou (2018GZ09), the Science and Technology support Program of Suzhou (SNG2017048), the Natural Science Fund for Colleges and Universities in Jiangsu Province (No. 18KJB230004), the Fund of SuZhou University of Science and Technology (XKZ2017001), the QingLan Project of Jiangsu Province, and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.
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These authors contributed equally: Jiahuan Hu, Mengxue Li, Zhengting Lu
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Hu, J., Li, M., Lu, Z. et al. The mechanism of damage in the midgut of Bombyx mori after chlorantraniliprole exposure. Ecotoxicology 28, 903–912 (2019). https://doi.org/10.1007/s10646-019-02089-z
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DOI: https://doi.org/10.1007/s10646-019-02089-z