Synopsis
To assess the genetic variation and population structure of wild chum salmon in China, we analyzed microsatellite loci for populations in the Amur, Wusuli, Suifen Current and the Tumen rivers. We evaluated expected heterozygosity with two estimators of genetic differentiation (F ST and G ST ) and Nei’s standard genetic distance. The average expected heterozygosity across the 10 loci was 0.65 in the Wusuli River and the Suifen Current River, 0.64 in the Amur River and 0.66 in the Tumen River, The results of this study show that the recent declines in chum salmon have not led to low levels of genetic variability in China. The proportion of inter-population subdivision among chum salmon was between 5.7 and 6.8%. According to the estimator used, the NJ tree based on Nei’s standard genetic distance indicated that there were two different branches (the Sea of Okhotsk branch and the Sea of Japan branch), the Amur River and the Wusuli River populations were closer, while the Suifen Current River and the Tumen River clustered together. The genetic test for population bottlenecks provided no evidence for a significant genetic signature of population decline, which is consistent with the record of the four populations we have in the last few years.
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References
A.O. Ball R.W. Chapman (2003) ArticleTitlePopulation genetic analysis of white shrimp, Litopenaeus setiferus, using microsatellite genetic markers Mol. Ecol. 12 2319–2330 Occurrence Handle12919471
M.A. Banks M.S. Blouin B.A. Baldwin V.K. Rashbrook H.A. Fitzgerald S.M. Blankenship D. Hedgecock (1999) ArticleTitleIsolation and inheritance of novel microsatellites in Chinook (Oncorhynchus tshawytscha) J. Hered. 90 281–288
Buchholz W., Miller S.J., Spearman W.J. (1999). Summary of PCR primers for salmonid genetic studies. Alaska Fisheries Progress Report Number 99–1
W.G. Buchholz S.J. Miller W.J. Spearman (2001) ArticleTitleIsolation and characterization of chum salmon microsatellite loci and use across species Anim. Gene. 3 162–165
V.A. Brykov O.N. Kirillova A.D. Kukhlevskii N.E. Poliakova L.A. Skurikhina (2000) ArticleTitleMitochondrial DNA variation in populations of the chum salmon Oncorhynchus keta (Walbaum) from rivers of the prymorye and Sakhalin Genetika 10 1388–1393
V.A. Brykov N.E. Poliakova A.V. Prokhorova (2003) ArticleTitlePhylogenic and geographic analysis of chum salmon Oncorhynchus keta (Walbaum) in Asian populations based on mitochondrial DNA variation Genetika 1 75–82
J.M. Cornuet G. Luikart (1996) ArticleTitleDescription and power analysis of two tests for detecting recent population bottlenecks from allele frequency data Genetics 144 2001–2014 Occurrence Handle1:STN:280:ByiC3s3mtlU%3D Occurrence Handle8978083
C.Z. Dong G.G. Li (1989) ArticleTitleA preliminary study on the population structure of salmon migration population in Suifen Current J. Fish. Chin. 2 124–132
C.Z. Dong J.M. Zhang (1993) ArticleTitleA preliminary study on the population ecology of salmon migration population in Suifen Current Marine Sci. Chin. 3 59–63
C.Z. Dong J. Wang (1999) ArticleTitleMigration population structure of chum salmon (Oncorhynchus ketaWalbaum) in Wusuli River J. Fish. Sci. Chin. 3 5–9
V.V. Efremov (2001) ArticleTitleGenetic variability and differentiation of populations of chum salmon Oncorhynchus keta (Walbaum) from the southern Russian Far East Genetika 3 365–372
J. Goudet (1995) ArticleTitleFSTAT, version 1.2: A computer program to calculate F-statistics J. Hered. 86 485–486
S.W. Guo E.A. Thompson (1992) ArticleTitlePerforming the exact test of Hardy–Weinberg proportions for multiple alleles Biometrics 48 361–372 Occurrence Handle1:STN:280:By2A2crltlY%3D Occurrence Handle1637966
Y. Han Y.S. Wang Z.T. Fan M. Liu (2002) ArticleTitleA survey on the resources of chum salmon(Oncorhynchus keta Walbaum) in Heilongjiang waters Chin. J. Fish. 15 24–34
K.S. Kim J.S. Yeo C.B. Choi (2002) ArticleTitleGenetic diversity of north-east Asian cattle based on microsatellite data Anim. Genet. 33 201–204 Occurrence Handle12030923
G. Luikart J.M. Cornuet (1998) ArticleTitleEmpirical evaluation of a test for identifying recently bottlenecked populations from allele frequency data Conserv. Biol. 12 228–237
M. Nei (1973) ArticleTitleAnalysis of gene diversity in subdivided populations, Proc Nat. Acad. Sci. USA 70 3321–3323
M. Nei (1978) ArticleTitleEstimation of average heterozygosity and genetic distance from a small number of individuals Genetics 89 583–590
M. Nei (1983) Genetic polymorphism and the role of mutation in evolution M. Nei R. Koehn (Eds) Evolution of Genes and Proteins Sinauer Associates Sunderland, UK 165–190
M. Nei (1987) Molecular Evolution Genetics Columbia University press New York
J.B. Olsen W.J. Spearman G.K. Sage S.J. Miller B.G. Flannery J.K. Wenburg (2004) ArticleTitleVariation in the population structure of Yukon River chum and coho salmon: evaluating the potential impact of localized habitat degradation Trans. Am. Fish. Soc. 133 476–483
Ota T. (1993). DISPAN: Genetic Distance and Phylogenetic Analysis. Pennsylvania State University, University Park, Pennsylvania, USA, pp
L.K. Park M.A. Brainard D.A. Dightman G.A. Winans (1993) ArticleTitleLow levels of intraspecific variation in the mitochondrial DNA of chum salmon (Oncorhynchus keta) Mol. Marine Biol. Biotechnol. 6 362–370
M. Raymond F. Rousset (1995) ArticleTitleGENEPOP (version 1.2): Population genetics software for exact tests and ecumenicism J. Hered. 86 248–249
A. Reilly N.G. Elliott P.M. Grewe C. Clabby P. Powell R.D. Ward (1999) ArticleTitleGenetic differentiation between Tasmanian cultured Atlantic salmon (Salmo salar L.) and their ancestral Canadian population: Comparison of microsatellite DNA and allozyme and mitochondrial DNA variation Aquaculture 173 459–469
W.R. Rice (1989) ArticleTitleAnalyzing tables of statistical tests Evolution 43 223–225
N. Saitou M. Nei (1987) ArticleTitleThe neighbor-joining method: a new method for reconstructing phylogenetic trees Mol. Biol. Evol. 4 406–425 Occurrence Handle1:STN:280:BieC1cbgtVY%3D Occurrence Handle3447015
S. Sato H. Kojima J. Ando H. Ando R.L. Wilmot L.W. Seeb V. Efremov L. LeClair W. Buchholz Deuk-Hee Jin S. Urawa M. Kaeriyama A. Urano S. Abe (2004) ArticleTitleGenetic population structure of chum salmon in the Pacific Rim inferred from mitochondrial DNA sequence variation Environ. Biol. Fish. 69 37–50
K.T. Scribner P.A. Crane W.J. Spearman L.W. Seeb (1998) ArticleTitleDNA and allozyme markers provide concordant estimates of population differentiation: Analyses of U.S. and Canadian populations of Yukon River fall-run chum salmon (Oncorhynchus keta) Can. J. Fish. Aquat. Sci. 55 1748–1758
L.W. Seeb P.A. Crane C.M. Kondzela R.L. Wilmot S. Urawa N.V. Varnavskaya J.E. Seeb (2004) ArticleTitleMigration of Pacific Rim chum salmon on the high seas: insights from genetic data Environ. Biol. Fish. 69 21–36
C.T. Smith B.F. Koop R.J. Nelson (1998) ArticleTitleIsolation and characterization of coho salmon (Oncorhynchus kisutch) microsatellites and their use in other salmonids Mol. Ecol. 11 1614–1616
N. Takezaki M. Nei (1996) ArticleTitleGenetic distances and reconstruction of phylogenetic trees from microsatellite DNA Genetics 144 389–399 Occurrence Handle8878702
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Chen, JP., Sun, DJ., Dong, CZ. et al. Genetic analysis of four wild chum salmonOncorhynchus keta populations in China based on microsatellite markers. Environ Biol Fish 73, 181–188 (2005). https://doi.org/10.1007/s10641-004-6114-0
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DOI: https://doi.org/10.1007/s10641-004-6114-0