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LncRNA SNHG7 Promotes the HCC Progression Through miR-122-5p/FOXK2 Axis

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Abstract

Hepatocellular carcinoma (HCC) is a malignant tumor with high mortality and severe complication in China. Numerous studies have shown that long noncoding RNAs (lncRNAs) are involved in the regulation of various processes in cancer cells. Our research aimed to investigate the underlying mechanism of the lncRNA small nucleolar RNA host gene 7 (SNHG7) in HCC development. The expression of SNHG7, microRNA-122-5p (miR-122-5p), and Forkhead box K2 (FOXK2) was assessed via quantitative real-time polymerase chain reaction. 3-(4,5) -dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) and transwell assays were performed to measure cell viability, migration, and invasion, respectively. The relative protein levels were detected by Western blot. The relationships between miR-122-5p and SNHG7 or FOXK2 were predicted by online software and then confirmed by dual-luciferase reporter assay. Animal experiments were conducted to clarify the effects of SNHG7 on proliferation in vivo. To begin with, SNHG7 was upregulated, while miR-122-5p was downregulated in HCC tissues and cells. Downregulation of SNHG7 inhibited cell growth and metastasis. Interestingly, SNHG7 could abolish the effects of miR-122-5p on HCC cells. Furthermore, miR-122-5p targeted FOXK2 and miR-122-5p recovered the effects of FOXK2 downregulation on cell growth and metastasis in HCC cells. Besides, SNHG7 facilitated HCC tumor growth in vivo through the miR-122-5p/FOXK2 axis. The lncRNA SNHG7 boosted the development of HCC by regulating FOXK2 through sponging miR-122-5p.

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Authors and Affiliations

  1. Department of Infectious Diseases, The First Hospital of Lanzhou University, No. 1 Donggang West Road, Chengguan District, Lanzhou City, Gansu, China

    Zhengbin Zhao

  2. Department of Laboratory, Hospital of Northwest Minzu University, Lanzhou, 730030, Gansu, China

    Jing Gao

  3. Medical College of Northwest Minzu University, Lanzhou, 730030, Gansu, China

    Shuangsheng Huang

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  1. Zhengbin Zhao
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  2. Jing Gao
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  3. Shuangsheng Huang
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Correspondence to Zhengbin Zhao.

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Zhao, Z., Gao, J. & Huang, S. LncRNA SNHG7 Promotes the HCC Progression Through miR-122-5p/FOXK2 Axis. Dig Dis Sci 67, 925–935 (2022). https://doi.org/10.1007/s10620-021-06918-2

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  • DOI: https://doi.org/10.1007/s10620-021-06918-2

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