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Restriction Fragment Length Polymorphism of Urease C and Urease B Genes of Helicobacter pylori Strains Isolated from Brazilian Patients with Peptic Ulcer and Chronic Gastritis

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Abstract

The aim of the present work is to identify the presence of Helicobacter pylori bacterium in samples of gastric mucosa fragments, obtained by gastric biopsy, from Brazilian patients with peptic ulcer and chronic gastritis and also to determine differences among the prevalent strains in these two diseases by urease C and urease B genes amplification utilizing nested polymerase chain reaction (PCR) and PCR. We encountered 17 genotyping patterns for urease C and 7 for urease B and, although no significant differences were found among the patterns encountered for both diseases, we found predominant groups for each disease. Typing methods of the products obtained by nested PCR and PCR show a functional scheme and are of great importance for epidemiologic studies and H. pylori strain characterization, in addition to allowing correlation among the several strains and their role in the diseases caused by this microorganism.

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Acknowledgments

This work was supported by CAPES – Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.

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Authors and Affiliations

  1. Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas - UNICAMP, 13083-970, Campinas, SP, Brazil

    Bruna Maria Röesler & Telma Barbosa de Oliveira

  2. Department of Clinical Medicine, Faculty of Medical Sciences, State University of Campinas - UNICAMP, 13083-970, Campinas, SP, Brazil

    Sandra Helena Alves Bonon & Sandra Cecília Botelho Costa

  3. Center of Diagnosis of Digestive Diseases – Gastrocenter, Faculty of Medical Sciences, State University of Campinas - UNICAMP, 13083-970, Campinas, SP, Brazil

    José Murilo Robilotta Zeitune

  4. Section of Digestive Endoscopy – Gastrocenter, Faculty of Medical Sciences, State University of Campinas - UNICAMP, 13083-970, Campinas, SP, Brazil

    Leonardo Trevisan Monici

Authors
  1. Bruna Maria Röesler
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  2. Telma Barbosa de Oliveira
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  3. Sandra Helena Alves Bonon
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  4. Leonardo Trevisan Monici
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  5. José Murilo Robilotta Zeitune
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  6. Sandra Cecília Botelho Costa
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Correspondence to Sandra Cecília Botelho Costa.

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Röesler, B.M., de Oliveira, T.B., Bonon, S.H.A. et al. Restriction Fragment Length Polymorphism of Urease C and Urease B Genes of Helicobacter pylori Strains Isolated from Brazilian Patients with Peptic Ulcer and Chronic Gastritis. Dig Dis Sci 54, 1487–1493 (2009). https://doi.org/10.1007/s10620-008-0525-2

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  • DOI: https://doi.org/10.1007/s10620-008-0525-2

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