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Antidiabetic effects of novel cell culture established from grapevine, Vitis vinifera cv. Koshu

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Abstract

Vitis vinifera cv. Koshu is an indigenous cultivar in Japan and has several characteristics that distinguish it from European V. vinifera. In Japan, Koshu is the most popular cultivar for wine making. We report herein a cell culture established from Koshu for use as a system for the production of resveratrol and its derivatives. Grape cell culture YU-1 was developed from the apex tissues of Koshu. YU-1 growth was favorably compared with BY-2 growth, a standard cell line in plant cell biology. Stilbene production and stilbene synthesis gene expression in YU-1 were upregulated by UV-C irradiation. YU-1 irradiated with UV-C decreased hemolymph sugar levels in model animals. Taken together, this study suggests that YU-1 may be used as a source of valuable medicinal components in plant cell bioreactor systems.

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Acknowledgements

We thank Dr. Hironori Kobayashi and Dr. Ryoji Takata (Kirin Company, Ltd.) for valuable discussion.

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Authors and Affiliations

  1. Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Hiroshima, Japan

    Keiko Fujita

  2. Laboratory of Fruit Genetic Engineering, The Institute of Enology and Viticulture, University of Yamanashi, Kofu, Yamanashi, 400-0005, Japan

    Yoshinao Aoki & Shunji Suzuki

Authors
  1. Keiko Fujita
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  2. Yoshinao Aoki
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  3. Shunji Suzuki
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Correspondence to Shunji Suzuki.

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Fujita, K., Aoki, Y. & Suzuki, S. Antidiabetic effects of novel cell culture established from grapevine, Vitis vinifera cv. Koshu. Cytotechnology 70, 993–999 (2018). https://doi.org/10.1007/s10616-018-0203-y

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  • DOI: https://doi.org/10.1007/s10616-018-0203-y

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