Abstract
Magnetic field has been widely used in clinical diagnostics or for clinical treatment and is an important biomedical technology. Glioblastoma multiforme U87 and U251 are models of a fast growing malignant cancer. We focused on cellular level drafting of these cell lines as a time-dependent effect indicator of static magnetic fields (2000 ± 600 Gauss) by using their fast-growing properties. Cell viability showed a significant decrease (p < 0.01). The results coincided with the occurrence of apoptotic signals or protein expression of cyclin B1 and cyclin dependent kinase 1 in a non-apoptotic manner. Cdk1 was decreased in proportion to ankyrin G and cyclin B1 (Chi-square test, p = 0.0366). Our findings suggest that static magnetic stimulation creates a specific cyto-proliferative pattern, rather than producing randomized growth impairment.
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Acknowledgments
We thank Dr. Sung-hye Park in the Dept. of Pathology at Seoul National University Hospital for advice in cell morphology screening. This work was supported by National Research Foundation of Korea (NRF) grants funded by the Korean Government (MSIP) (NRF-20151009350, KOFAC-URP-2009). The authors declare no competing interests.
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Supplementary Fig. 1
Diagram of application of static magnetic fields in cultured human glioblastoma cell. The magnets were applied to the bottom of the wells with a distance of 0.1 - 0.3 cm to the cells. The cells were cultured on a plastic shelf (75-T, 24-well and 96-well respectively) 4.0 ± 0.2 cm above the metal shelf in the incubator, so the metal shelf does not influence the magnetic field within the wells. (JPEG 98 kb)
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Kim, S.C., Im, W., Shim, J.Y. et al. Static magnetic field controls cell cycle in cultured human glioblastoma cells. Cytotechnology 68, 2745–2751 (2016). https://doi.org/10.1007/s10616-016-9973-2
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DOI: https://doi.org/10.1007/s10616-016-9973-2