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Flavonoids from Heliotropium subulatum exudate and their evaluation for antioxidant, antineoplastic and cytotoxic activities II

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Abstract

The flavonoids are the largest group of phenolic compounds isolated from a wide range of higher plants. These compounds work as antimicrobials, anti-insect agents and protect plants from other types of biotic and abiotic stresses. Various researchers have suggested that flavonoids possessed antioxidant, antineoplastic and cytotoxic activities. The main objective of this study was to test dichloromethane fraction of resinous exudate of Heliotropium subulatum for their antioxidant, antineoplastic and cytotoxic activities, as well as to search new antioxidant and antineoplastic agents for pharmaceutical formulations. Five flavonoids were isolated from resinous exudate of this plant species and screened for their in vitro and in vivo antioxidant models (DPPH radical scavenging, reducing power, superoxide anion scavenging, metal chelating scavenging systems, catalase and lipid peroxidation), antineoplastic (Sarcoma 180), and cytotoxic (Chinese hamster V79 cells) activities. Tricetin demonstrated maximum antioxidant activity against both in vitro and in vivo experimental systems while galangin exhibited maximum inhibition (78.35%) at a dose of 10 µg/kg/day against Sarcoma 180. Similarly, it was found that galangin also showed highest activity (21.1 ± 0.15%) at a concentration of 70 µg/ml to Chinese hamster V79 cells. The observed results suggest that tricetin has a potential to scavenge free radicals in both in vitro and in vivo models while the galangin could be considered as antitumor and cytotoxic agent.

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Authors’ contribution

BS and PMS designed and perform the experimental work and analyzed the data. RAS reviewed the manuscript and facilitated the help in equipments and chemicals. We are grateful to Professor P. Singh, Department of Chemistry, University of Rajasthan, Jaipur for providing the standard compounds and Professor Dr. H. Itokawa, College of Pharmacy, Tokyo, for his kind help in the screening process of antineoplastic and cytotoxic activities as well as Dr. H. Singh, Pacific University for helping in study of in vivo antioxidant activities.

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Authors and Affiliations

  1. Institute of Biotechnology, Amity University Rajasthan, Jaipur, 303002, India

    Bharat Singh

  2. Medicinal Plants Research Laboratory, Department of Botany, University of Rajasthan, Jaipur, 302004, India

    Pooran M. Sahu & Ram A. Sharma

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  1. Bharat Singh
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  2. Pooran M. Sahu
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Correspondence to Bharat Singh.

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Singh, B., Sahu, P.M. & Sharma, R.A. Flavonoids from Heliotropium subulatum exudate and their evaluation for antioxidant, antineoplastic and cytotoxic activities II. Cytotechnology 69, 103–115 (2017). https://doi.org/10.1007/s10616-016-0041-8

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