Skip to main content
SpringerLink
Log in

Selective fraction of Atractylodes lancea (Thunb.) DC. and its growth inhibitory effect on human gastric cancer cells

  • Brief Report
  • Published:
Cytotechnology Aims and scope Submit manuscript

Abstract

The purpose of this study was to investigate the pharmacological effect of fraction of Atractylodes lancea (Thunb.) DC. (A. lancea) extract. In this study, we isolated different polarity fractions, including petroleum ether (PE), ethyl acetate, n-butanol, and the remaining H2O fractions from the water extract of A. lancea. The antigastric cancer properties of the different fractions in BGC-823 and SGC-7901 cells were evaluated. Apoptotic cells were treated with PE fraction and stained with Hoechst 33342 and 5,5,6,6-tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide. The cell cycle was analyzed via flow cytometry. The main compounds of PE fraction were determined by HPLC–ESI–MS. Results of this study showed that the PE fraction of A. lancea inhibited the growth of BGC-823 and SGC-7901 cells in a dose- and time-dependent manner. The morphological and mitochondrial transmembrane potential changes suggested that the cells showed preliminary apoptosis characteristics after treatment with the three different polarities. The main compounds of PE fraction include two sesquiterpene compounds: eudesm-4(15),7-diene-9α,11-diol and eudesm-4(15)-ene-7a,11-diol; three sesquiterpene lactone compounds: atractylenolid I, atractylenolid III and 3-β-acetyl-atractylenolid III and one polyacetylenic compound: 4,6,12-tetradecatriene-8,10-diyne-1,3,14-triol.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

References

  • Adaramoye OA, Sarkar J, Singh N (2011) Antiproliferative action of Xylopia aethiopica fruit extract on human cervical cancer cells. Phytother Res 25:1558–1563

    Article  Google Scholar 

  • Chen ZL, Cao WY, Zhou G (1997) A sesquiterpene lactam from Atractylodes macrocephala. Phytochemistry 45:765–767

    Article  CAS  Google Scholar 

  • Correa P, Shiao YH (1994) Phenotypic and genotypic events in gastric carcinogenesis. Cancer Res 54:1941–1943

    Google Scholar 

  • Crew KD, Neugut AI (2006) Epidemiology of gastric cancer. World J Gastroenterol 12:354–362

    Google Scholar 

  • Ding H, Wu YC, Lin HC (2000) Phytochemical and pharmacological studies on Chinese Changzhu. J Chin Chem Soc 47:561–566

    CAS  Google Scholar 

  • Dobos GJ, Tan L, Cohen MH (2005) Are national quality standards for traditional Chinese herbalmedicine sufficient? Current governmental regulations for traditional Chinese herbal medicine in certain Western countries and China as the Eastern origin country. Complementary Ther Med 13:183–190

    Article  CAS  Google Scholar 

  • Endo K, Taguchi T, Taguchi F (1979) Antiinflammatory principles of Atractylodes rhizomes. Chem Pharm Bull 27:2954–2958

    Article  CAS  Google Scholar 

  • Ferlay J, Shin HR, Bray F (2010) Estimates of wordwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer 127:2893–2917

    Article  CAS  Google Scholar 

  • Gatti L, Perego P (2009) Cellular resistance to oxaliplatin and drug accumulation defects. Cancer Drug Discov Dev 2:115–124

    Google Scholar 

  • Kano Y, Komatsu KI, Saito KI (1989) A new polyacetylene compound from Atractylodes rhizome. Chem Pharm Bull 37:193–194

    Article  CAS  Google Scholar 

  • Kitajima J, Kamoshita A, Ishikawa T (2003) Glycosides of Atractylodes lancea. Chem Pharm Bull 51:673–678

    Article  CAS  Google Scholar 

  • Kubo M, Nogami M, Nishimura M (1983) Studies of crude drugs about its origin, process and quality. I. The preventive effects of Chinese crude drug ‘Zhu’ on experimental stomach ulcer and its pharmacological evaluation (1). Yakugaku Zasshi 103:442–448

    CAS  Google Scholar 

  • Li X, Wang JH, Li X (2002) Studies on the chemical consitutent of Atractylodes chinensis (DC.) Koidz. J Shengyang Pharm Univ 9:178

    Google Scholar 

  • Lin HP, Kuo LK, Chuu CP (2012) Combined treatment of curcumin and small molecule inhibitors suppresses prolif-eration of A549 and H1299 human non-small-cell lung cancer cells. Phytother Res 26:122–126

    Article  CAS  Google Scholar 

  • Luan JL, Duan HY, Liu Q (2010) Inhibitory effects of norcantharidin against human lung cancer cell growth and migration. Cytotechnology 62:349–355

    Article  CAS  Google Scholar 

  • Meng H, Li GY, Dai RH (2010) Chemical constituents of Atractylodes chinensis (DC.) Koidz. Biochem Syst Ecol 38:1220–1223

    Article  CAS  Google Scholar 

  • Nakai Y, Kido T, Hashimoto K, Kase Y, Sakakibara I, Higuchi M, Sasaki H (2003) Effect of the rhizomes of Atractylodes lancea and its constituents on the delay of gastric emptying. Ethnopharmacology 84:51–55

    Article  Google Scholar 

  • Naoyoshi N, Mari I, Mari K (2012) In vitro cytotoxic effect of ethanol extract prepared from Sporophyll of Undaria pinnatifida on human colorectal cancer cells. Phytother Res 26:191–196

    Article  Google Scholar 

  • Nogami M, Moriura T, Kubo M, Tani T (1986) Studies on the origin, processing and quality of crude drugs. II. Pharmacological evaluation of the Chinese crude drug ‘zhu’ in experimental stomach ulcer (2). Inhibitory effect of extract of Atractylodes lancea on gastric secretion. Chem Pharm Bull (Tokyo) 34:3854–3860

    Article  CAS  Google Scholar 

  • Parkin DM, Bray F, Ferlay J, Pisani P (2005) Global Cancer Statistics, 2002. Cancer J Clin 55:74–108

    Google Scholar 

  • Ruan WJ, Lai MD, Zhou JG (2006) Anticancer effects of Chinese herbal medicine, science or myth? J Zhejiang Univ SCIENCE B 12:1006–1014

    Article  Google Scholar 

  • Tang W, Eisenbrand G (1992) Chinese drugs of plant origin: chemistry, pharmacology, and use in traditional and modern medicine. Springer, Berlin and New York

    Book  Google Scholar 

  • Wang CC, Chen LG, Yang LL (2002) Cytotoxic activity of sesquiterpenoids from Atractylodes ovata on leukemia cell lines. Planta Med 68:204–208

    Article  CAS  Google Scholar 

  • Wang CC, Lin SY, Cheng HC (2006) Pro-oxidant and cytotoxic activities of atractylenolide I in human promyeloleukemic HL-60 cells. Food Chem Toxicol 8:1308–1315

    Article  Google Scholar 

  • Wang LY, Duan JA, Qian SH (2007) Studies on the chemical consitutent of Atractylodes lancea (Thunb.) DC. Chin Tradit Herbal Drugs 38:499–500

    Google Scholar 

  • Wang CH, Wang SC, Chen QH (2008a) A capillary gas chromatography-selected ion monitoring mass spectrometry method for the analysis of atractylenolide I in rat plasma and tissues, and application in a pharmacokineticstudy. Chromatogr B Analyt Technol Biomed Life 3:215–222

    Article  Google Scholar 

  • Wang HX, Liu CM, Liu Q (2008b) Three types of sesquiterpenes from rhizomes of Atractylodes lancea. Phytochemistry 69:2088–2094

    Article  CAS  Google Scholar 

  • Wang LP, Duan HY, Wang YS (2010) Inhibitory effects of Lang-du extract on the in vitro and in vivo growth of melanoma cells and its molecular mechanisms of action. Cytotechnology 62:357–366

    Article  Google Scholar 

  • Wu X, Han RB, Luo HS (2004) Studies on the chemical constituents of Actractycodes japonica Koidz ex Kitam. J Yanbian Univ (Nat Sci) 30:29–34

    CAS  Google Scholar 

  • Yahara S, Higashi T, Iwaki K (1989) Studies on the constituents of Atractylodes lancea. Chem Pharm Bull 37:2995–3000

    Article  CAS  Google Scholar 

  • Yuan ZH, Huang MH, Chen Yuan Y (2011) Kind of treatment of liver, stomach, lung, and cancer of the blood and preparation methods that combination of Chinese herbal medicine, CN102205059A (in Chinese)

  • Zhang LL, Zhang SL (2011) Modulating Bcl-2 family proteins and caspase-3 in induction of apoptosis by paeoniflo-rin in human cervical cancer cells. Phytother Res 25:1551–1557

    Article  CAS  Google Scholar 

  • Zhang YQ, Xu SB, Lin C (1999) Gastrointestinal inhibitory effects of sesquiterpene lactones from Atractylodes macrocephala. Zhong Yao Cai 12:636–640

    Google Scholar 

Download references

Acknowledgments

This work is supported by Bureau of traditional Chinese medicine of Jiangsu Province (No. LZ09086; HZ07005).

Conflict of interest

The authors have declared that there is no conflict of interest.

Author information

Authors and Affiliations

  1. School of Pharmaceutical Science, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu province, People’s Republic of China

    Ming Zhao, Qingqing Wang, Zhen Ouyang, Bangxing Han, Wenbing Wang, Yuan Wei, Yan Wu & Bing Yang

Authors
  1. Ming Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qingqing Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhen Ouyang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bangxing Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wenbing Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yuan Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yan Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Bing Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhen Ouyang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhao, M., Wang, Q., Ouyang, Z. et al. Selective fraction of Atractylodes lancea (Thunb.) DC. and its growth inhibitory effect on human gastric cancer cells. Cytotechnology 66, 201–208 (2014). https://doi.org/10.1007/s10616-013-9559-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10616-013-9559-1

Keywords

Search

Navigation