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Cell biology of deep-sea multicellular organisms

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Abstract

Establishing tissue cultures derived from deep-sea multicellular organisms has been extremely difficult because of the serious damage they sustain upon decompression and exposure to the high temperature of surface seawater. We developed a novel pressure-stat aquarium system for the study of living deep-sea multicellular organisms under pressure. Using this system, we have succeeded in maintaining a variety of deep-sea multicellular organisms under pressure and atmospheric conditions after gradual, slow decompression. Furthermore, we successfully cultivated and freeze-stocked pectoral fin cells of the deep-sea eel Simenchelys parasiticus collected at a depth of 1,162 m under atmospheric pressure conditions. This review describes novel capture and maintenance devices for deep-sea organisms and cell culture studies of the organisms under atmospheric and pressure conditions.

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References

  • Cecil JT (1969) Mitosis in cell cultures from cardiac tissue of the surf clam Spisula solidissima. J Invest Pathol 14:407–410

    Article  CAS  Google Scholar 

  • Fujiwara Y, Tsukahara J, Hashimoto J et al (1998) In situ spawning of a deep-sea vesicomyid clam: evidence for an environmental cue. Deep-Sea Res I 45:1881–1889

    Article  Google Scholar 

  • Gibbs AG (1997) Biochemistry at depth. In: Randall DJ, Farrell AP (eds) Deep-sea fishes. Academic Press, San Diego, pp 239–277

    Google Scholar 

  • Giese AC, Kanatani H (1987) Maturation and spawning. In: Giese AC, Pearse JS, Pearse VB (eds) General aspects: seeking unity in diversity. Reproduction of marine invertebrates, vol. 9. Blackwell Scientific Publications and Boxwood Press, Palo Alto and Pacific Grove, California, pp 251–329

    Google Scholar 

  • Hashimoto J, Fujikura K, Aoki T et al (1992) Development of a suction sampler (slurp gun) for deep sea organisms. Proc JAMSTEC Symp Deep Sea Res 8:367–372

    Google Scholar 

  • Horikoshi K, Tsujii K (1999) Extremophiles in deep-sea environments. Springer, Tokyo

    Google Scholar 

  • Koyama S (2003) Acclimatization for deep-sea multicellular organisms under atmospheric pressure. Biosci Ind 61:36–37

    Google Scholar 

  • Koyama S, Aizawa M (2000) Tissue culture of the deep-sea bivalve Calyptogena soyoae. Extremophiles 4:385–389

    Article  CAS  Google Scholar 

  • Koyama S, Miwa T, Sato T et al (2001) Optical chamber system designed for microscopic observation of living cells under extremely high hydrostatic pressure. Extremophiles 5:409–415

    Article  CAS  Google Scholar 

  • Koyama S, Miwa T, Horii M et al (2002) Pressure-stat aquarium system designed for capturing and maintaining deep-sea organisms. Deep-Sea Res I 49:2095–2102

    Article  Google Scholar 

  • Koyama S, Horii M, Miwa T et al (2003a) Tissue culture of the deep-sea eel Simenchelys parasiticus collected at 1,162 m. Extremophiles 7:245–248

    Article  CAS  Google Scholar 

  • Koyama S, Horii M, Miwa T et al (2003b) Erratum. Extremophiles 7:340

  • Koyama S, Nagahama T, Ootsu N et al (2005a) Survival of deep-sea shrimp (Alvinocaris sp.) during decompression and larval hatching at atmospheric pressure. Mar Biotechnol 7:272–278

    Article  CAS  Google Scholar 

  • Koyama S, Kobayashi H, Inoue A et al (2005b) Effects of the piezo-tolerance of cultured deep-sea eel cells on survival rates, cell proliferation, and cytoskeletal structures. Extremophiles 9:449–460

    Article  Google Scholar 

  • Li MF, Stewart JE (1966) In vitro cultivation of cells of the oyster, Crassostrea virginica. J Fish Res BD Canada 23:595–599

    Google Scholar 

  • Macdonald AG, Gilchrist I (1978) Further studies on the pressure tolerance of deep-sea crustacea, with observations using a new high-pressure trap. Mar Biol 45:9–21

    Article  Google Scholar 

  • Momma H, Mitsuzawa K, Kaiho Y et al (1995) Long-term deep sea floor observation off Hatsushima Island in Sagami Bay—one year in the Calyptogena soyoae clam colony. JAMSTEC J Deep Sea Res 11:249–268

    Google Scholar 

  • Nakabo T (2000) Fishes of Japan with pictorial keys to the species, 2nd edn. Tokai University Press, Tokyo

    Google Scholar 

  • Odintsova NA, Khomenko AV (1991) Primary cell culture from embryos of the Japanese scallop Mizuchopecten yessoensis (bivalvia). Cytotechnology 6:49–54

    Article  CAS  Google Scholar 

  • Siebenaller JF, Somero GN (1979) Pressure-adaptive differences in the binding and catalytic properties of muscle-type (M4) lactate dehydrogenases of shallow- and deep-living marine fishes. J Comp Physiol B 129:295–300

    Article  CAS  Google Scholar 

  • Smith KL Jr, Baldwin RJ (1997) Laboratory and in situ methods for studying deep-sea fishes. In: Randall DJ, Farrell AP (eds) Deep-sea fishes. Academic Press, San Diego, pp 351–378

    Google Scholar 

  • Takeuchi Y, Inoue K, Miki D et al (1999) Cultured mussel foot cells expressing abyssal protein genes. J Exp Zool 283:131–136

    Article  CAS  Google Scholar 

  • Van Dover CL (1994) In situ spawning of hydrothermal vent tubeworms (Riftia pachyptila). Biol Bull 186:134–135

    Article  Google Scholar 

  • Van Dover CL (2000) The ecology of deep-sea hydrothermal vents. Princeton University Press, Princeton, New Jersey

    Google Scholar 

  • Wilson RR Jr, Smith KL Jr (1985) Live capture, maintenance and partial decompression of a deep-sea grenadier fish (Coryphaenoides acrolepis) in a hyperbaric trap-aquarium. Deep-Sea Res 32:1571–1582

    Article  Google Scholar 

  • Yayanos AA (1978) Recovery and maintenance of live amphipods at a pressure of 580 bars from an ocean depth of 5,700 m. Science 200:1056–1059

    Article  Google Scholar 

Download references

Acknowledgments

The author is grateful to Dr. Masuo Aizawa (Tokyo Institute of Technology) and Dr. Koki Horikoshi (JAMSTEC) for helpful support and advice. The author also thanks Dr. Tetsuya Miwa for the gift of a deep-sea fish photograph.

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Authors and Affiliations

  1. Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, 237-0061, Japan

    Sumihiro Koyama

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  1. Sumihiro Koyama
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Correspondence to Sumihiro Koyama.

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Koyama, S. Cell biology of deep-sea multicellular organisms. Cytotechnology 55, 125–133 (2007). https://doi.org/10.1007/s10616-007-9110-3

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  • DOI: https://doi.org/10.1007/s10616-007-9110-3

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