Skip to main content
SpringerLink
Log in

The Effect of α-pinene from Pinus densiflora S. and a Polysaccharide from Angelica gigas Nakai on Differentiation and Proliferation of Human Embryonic Stem Cells

  • Published:
Cytotechnology Aims and scope Submit manuscript

Abstract

The leukemia inhibitory factor (LIF), which is a very expensive reagent, can be used to efficiently control the differentiation of human embryonic stem (ES) cells at concentrations >1000 units/ml for 6–7 days. However, in supplement <500 units/ml, most ES cells differentiate within 3–4 days in in vitro cultures. α-Pinene from Pinus densiflora S. and a polysaccharide (MW 25 kDa) from A. gigas Nakai showed promising results as a substitute for LIF in cultivating ES cells. By adding both 0.5 (μg/ml) of α-pinene and the polysaccharide, most of the ES cells could be maintained under undifferentiated conditions after adding only 100 units/ml of LIF. It was found that α-pinene can play a role in preventing the ES cells from differentiating and the polysaccharide can be used to grow the ES cells. The results suggest that human ES cells can be maintained under undifferentiated conditions by supplementing both plant extracts, which can result in a reduction in the amount of LIF needed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • K.S. Ahn W.S. Sim I.H. Kim (1996) ArticleTitleDecursin a cytotoxic agent and protein kinase C activator from the root of Angelica gigas Planta Med. 62 7–9 Occurrence Handle8720379 Occurrence Handle1:STN:280:DyaK28zlt1Cmuw%3D%3D

    PubMed  CAS  Google Scholar 

  • K.S. Ahn W.S. Sim H.M. Kim S.B. Han H.H. Kim (1998) ArticleTitleImmuno- stimulating polysaccharide from cell culture of A. gigas Nakai Biotech Lett. 20 5–7 Occurrence Handle1:CAS:528:DyaK1cXhtVagur8%3D Occurrence Handle10.1023/A:1005310609619

    Article  CAS  Google Scholar 

  • R.D. Allsopp H. Vaziri C. Patterson S. Goldstein E.V. Younglai A.B. Futcher C.B. Harley (1992) ArticleTitleTelomere length predicts replicative capacity of human fibroblasts Proc. Natl. Acad. USA 89 10114–10118 Occurrence Handle1:CAS:528:DyaK3sXhvF2luw%3D%3D Occurrence Handle10.1073/pnas.89.21.10114

    Article  CAS  Google Scholar 

  • P. Altman (1989) ArticleTitleAustralian tea tree oil Aust. J. Biotechnol 3 217–248

    Google Scholar 

  • P.W. Andrews (1981) ArticleTitlePluripotent cell line from early mouse embryos cultured in medium conditioned by tetracarcinoma stem cells Proc. Natl. Acad. Sci. USA 78 7634–7638 Occurrence Handle10.1073/pnas.78.12.7634

    Article  Google Scholar 

  • R.O. Becker (2002) ArticleTitleInduced dedifferentiation: a possible alternative to embryonic stem cell transplants NeuroRehabilitation 17 23–31 Occurrence Handle12016344

    PubMed  Google Scholar 

  • A. Belldegrun A. Kasid M. Uppenkamp S. Topalian S. Rosenberg (2001) ArticleTitle In vitro cultivation of human ES cells J. Immunol 142 4520–4526

    Google Scholar 

  • V. Brower (2001) ArticleTitleTechnologies for regenerative medicine Genet. Eng. News 21 8–12

    Google Scholar 

  • M.J. Evans M. Kaufman (1981) ArticleTitleEstablishment in culture of pluripotential stem cells from mouse embryos Nature 292 151–156

    Google Scholar 

  • C.Y. Fong A. Bongso (1999) ArticleTitleComparison of human blastulation rates and total cell number in sequential culture media with and without co-culture Hum. Reprod. 14 774–781 Occurrence Handle10221713 Occurrence Handle1:STN:280:DyaK1M3js12jsA%3D%3D Occurrence Handle10.1093/humrep/14.3.774

    Article  PubMed  CAS  Google Scholar 

  • E. Gluckman J.K. Bullock (1997) ArticleTitleOutcomes of cord-blood transplantation from related and unrelated donors N. Eng. J. Med. 337 373–379 Occurrence Handle1:STN:280:DyaK2sznslOjsA%3D%3D Occurrence Handle10.1056/NEJM199708073370602

    Article  CAS  Google Scholar 

  • M.S. Ham S.S. Kim J.S. Hong J.H. Lee E.K. Chung H.Y. Lee (1996) ArticleTitleScreening and comparison of active substances of A. gigas Nakai produced in Kangwon and A. acutiloba produced in Japan Kor. J. Appl. Microbiol. Biotechnol. 24 624–629 Occurrence Handle1:CAS:528:DyaK2sXotl2ntA%3D%3D

    CAS  Google Scholar 

  • C.B. Harely A.B. Futcher C.W. Greider (1990) ArticleTitleTelomere shorten during ageing of human fibroblasts Nature 345 458–460 Occurrence Handle10.1038/345458a0

    Article  Google Scholar 

  • C.B. Harley B. Villeponteau (1995) ArticleTitleTelomeres and telomerase in aging and cancer Curr. Opin. Genet. Develop. 5 249–255 Occurrence Handle1:CAS:528:DyaK2MXkvFWisL0%3D Occurrence Handle10.1016/0959-437X(95)80016-6

    Article  CAS  Google Scholar 

  • B. Hogan R. Beddington E. Constantini E. Lacy (1994) Isolation, culture and manipulation of embryonic stem cells Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press Plainview, NY 254–290

    Google Scholar 

  • J.S. Hong J.N. Lee S.H. Kim K.Y. Park J.S. Cho H.Y. Lee (1999) ArticleTitleThe changes of growth patterns and production of BDNF in perfusioin cultivation of human neuroblastoma cells J. Microbiol. Biotechnol. 9 323–327 Occurrence Handle1:CAS:528:DyaK1MXkvFGjs74%3D

    CAS  Google Scholar 

  • M.S. Kallos B.A. Reynolds A.L. Vescovi L.A. Behie (1998) High Cell Density Growth of Mammalian Neural Stem Cells as Aggregates in Bioreactors Fluidization IX, Public Engineering Foundation New York 1–45

    Google Scholar 

  • K.Y. Kim P.M. Davidson H.J. Chung (2000) ArticleTitleAntimicrobial effectiveness of pine needles on foodborne illness bacteria J. Microbiol. Biotechnol. 10 227–232 Occurrence Handle10.1159/000054823

    Article  Google Scholar 

  • M.H. Kim W.T. Chung Y.K. Kim J.H. Lee H.Y. Lee (2001) ArticleTitleThe effect of the oil of A. rugosa Kuntze and three its components on human cancer cell lines J. Essent. Oil Res. 13 214–218 Occurrence Handle1:CAS:528:DC%2BD3MXksFantL4%3D

    CAS  Google Scholar 

  • N.W. Kim M.A. Piatyszek K.R. Prowse C.B. Harley M.D. West P.L.C. Ho G.M. Coviello W.E. Wright J.W. Shay (1994) ArticleTitleSpecific association of human telomerase activity with immortal cells and cancer Science 266 2011–2015 Occurrence Handle7605428 Occurrence Handle1:CAS:528:DyaK2MXisl2lu7g%3D

    PubMed  CAS  Google Scholar 

  • Y. Kumazawa K. Mizunoe Y. Otsuka (1982) ArticleTitleImmunostimulating polysaccharide separated from hot water extract of A. acutiloba. Immunology 47 75–83 Occurrence Handle6981588 Occurrence Handle1:CAS:528:DyaL38XlvFKlsL4%3D

    PubMed  CAS  Google Scholar 

  • J. Kurtzberg (1996) ArticleTitlePlacental blood as a source of hematopoitic stem cells for transplantation into unrelated recipients New. Engl. J. Med. 335 157–162 Occurrence Handle8657213 Occurrence Handle1:STN:280:DyaK283ot1Clug%3D%3D Occurrence Handle10.1056/NEJM199607183350303

    Article  PubMed  CAS  Google Scholar 

  • J.H. Li Y. Wei T.E. Wagner (1999) ArticleTitle In vitro endothelial differentiation of long-term cultured murine embryonic cells induced by matrigel Stem Cells 17 72–81 Occurrence Handle10195567 Occurrence Handle1:STN:280:DyaK1M3gvF2nsA%3D%3D Occurrence Handle10.1002/stem.170072

    Article  PubMed  CAS  Google Scholar 

  • V. Ling S. Neben (1997) ArticleTitleIn vitro differentiation of embryonic stem cells: Immunophenotypic analysis of cultured embryoid bodies J. Cell Physiol. 171 104–115 Occurrence Handle9119886 Occurrence Handle1:CAS:528:DyaK2sXisVOjsbw%3D Occurrence Handle10.1002/(SICI)1097-4652(199704)171:1<104::AID-JCP12>3.0.CO;2-G

    Article  PubMed  CAS  Google Scholar 

  • E. Marshall (1998) ArticleTitleA versatile cell line raises scientific hopes, legal questions Science 282 1014–1015 Occurrence Handle9841438 Occurrence Handle1:CAS:528:DyaK1cXntlant78%3D Occurrence Handle10.1126/science.282.5391.1014

    Article  PubMed  CAS  Google Scholar 

  • B.E. Reubinoff M.F. Pera C.Y. Fong A. Trounson A. Bongso (2000) ArticleTitleEmbryonic stem cell lines from human blastocysts: somatic differentiation in vitro Nature/Biotech. 18 399–404 Occurrence Handle1:CAS:528:DC%2BD3cXis1Gmt74%3D

    CAS  Google Scholar 

  • T.M. Rose D.M. Weiford N.L. Gunderson A.G. Bruce (1994) ArticleTitleOncostatin M inhibits the differentiation of pluropotent embryonic stem cells in vitro Cytokine 6 48–54 Occurrence Handle8003633 Occurrence Handle1:CAS:528:DyaK2cXitFGjsLw%3D Occurrence Handle10.1016/1043-4666(94)90007-8

    Article  PubMed  CAS  Google Scholar 

  • M. Schuldiner O. Yanuka J.E. Itskovitz D.A. Melton N. Benvenisty (2000) ArticleTitleEffect of eight growth factors on the differentiation of cells derived from human embryonic stem cells Proc. Natl. Acad. Sci. USA 97 11307–11312 Occurrence Handle11027332 Occurrence Handle1:CAS:528:DC%2BD3cXnsF2ruro%3D Occurrence Handle10.1073/pnas.97.21.11307

    Article  PubMed  CAS  Google Scholar 

  • M.J. Shamblott J. Axelman J.W. Littlefield P.D. Blumenthal G.R. Huggins Y., Cui L. Cheng J.D. Gearhart (2001) ArticleTitleHuman embryonic germ cell derivatives express a broad range of developmentally distinct markers and porliferate extensively in vitro Proc. Natl. Acad. Sci. USA 98 113–118 Occurrence Handle11134532 Occurrence Handle1:CAS:528:DC%2BD3MXkslKktw%3D%3D Occurrence Handle10.1073/pnas.021537998

    Article  PubMed  CAS  Google Scholar 

  • J.A. Thomson E.J. Itskovitz S.S. Shapiro M.A. Watknitz J.J. Swiergiel J.M. Jones (1998) ArticleTitleEmbryonic stem cell lines derived from human blastocysts Science 282 1145–1147 Occurrence Handle9804556 Occurrence Handle1:CAS:528:DyaK1cXntleisLg%3D Occurrence Handle10.1126/science.282.5391.1145

    Article  PubMed  CAS  Google Scholar 

  • J.A. Thomson J. Kalishman T.G. Golos M. Durning C.P. Harris J.P. Hearn (1995) ArticleTitleIsolation of a primate embryonic stem cell line Proc. Natl. Acad. Sci. USA 92 7844–7848 Occurrence Handle7544005 Occurrence Handle1:CAS:528:DyaK2MXnsFOnt7w%3D Occurrence Handle10.1073/pnas.92.17.7844

    Article  PubMed  CAS  Google Scholar 

  • N.L. Wang H. Kiyohara T. Matsumoto H. Ostuka H. Yamada (1994) ArticleTitlePoloyclonal antibody against a complement-activating pectin from the roots of A. acutiloba Planta Med. 60 425–429 Occurrence Handle7997470

    PubMed  Google Scholar 

  • J.P. Whish (1996) ArticleTitleA flexible distillation system for the isolation of essential oils J. Essent. Oil Res. 8 405–410 Occurrence Handle1:CAS:528:DyaK28XkvVWrtbo%3D

    CAS  Google Scholar 

  • K. Zhiming L. Zhengtao D. Bangyu (1995) ArticleTitleStudy on the antimutagenic effect of pine needle extract Mutat. Res. 347 103–109

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Biotechnology and Bioengineering, Kangwon National University, Chunchon, 200-701, Korea

    Seo H. Lee, Hyun Soo Lee, Hyung Cheol Moon, Dae Ho Kim, Young Shik Park & Hyeon Y. Lee

  2. Department of Biology, Chonnam National University, Gwangju, 500-271, Korea

    Baek Hwang

Authors
  1. Seo H. Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hyun Soo Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hyung Cheol Moon
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dae Ho Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Young Shik Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Baek Hwang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hyeon Y. Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hyeon Y. Lee.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, S.H., Lee, H.S., Moon, H.C. et al. The Effect of α-pinene from Pinus densiflora S. and a Polysaccharide from Angelica gigas Nakai on Differentiation and Proliferation of Human Embryonic Stem Cells. Cytotechnology 49, 87–94 (2005). https://doi.org/10.1007/s10616-004-0646-1

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10616-004-0646-1

Keywords

Search

Navigation