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Induction of the PAOX1-Promoter and Synthesis of GFP Protein in Wild X-33 Strain Recombinant Pichia pastoris Yeast Cells

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Abstract

Recombinant clones of X-33 strain Pichia pastoris containing the marker gene yEGFP were prepared. The optimal methanol concentration in the medium for induction of heterologous expression was determined in the recombinant clones.

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Authors and Affiliations

  1. S. Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Uzbekistan

    S. V. Polyarush & Sh. S. Azimova

  2. Institute of Biotechnology, Halle-Wittenberg University, Germany, 01620, Halle, Kurt-Mottestrasse, 3

    F. Hoffmann

Authors
  1. S. V. Polyarush
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  2. Sh. S. Azimova
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  3. F. Hoffmann
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Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 60–62, January–February, 2005.

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Polyarush, S.V., Azimova, S.S. & Hoffmann, F. Induction of the PAOX1-Promoter and Synthesis of GFP Protein in Wild X-33 Strain Recombinant Pichia pastoris Yeast Cells. Chem Nat Compd 41, 75–78 (2005). https://doi.org/10.1007/s10600-005-0078-y

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  • DOI: https://doi.org/10.1007/s10600-005-0078-y

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