Abstract
Eight polymorphic microsatellite loci are described for Littorina littorea (Linnaeus, 1758). Data on allelic variation in Irish and Celtic Sea samples are reported. The average number of alleles per locus was 11 (range 4–29), and observed and expected heterozygosities ranged from 6.9 to 84.3% and from 9.4 to 95.2%, respectively. Loci did not deviate from Hardy–Weinberg equilibrium and no linkage disequilibrium between loci pairs was detected. Microsatellites were not highly conserved in the congeners, L. fabalis, L. saxatilis, L. compressa and L. obtusata as evidenced by a low rate of cross-amplification. These microsatellites should prove useful in population genetic studies.
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Acknowledgments
C. McInerney was supported by a North–South strand 1 grant from the Higher Education Authority, Ireland to M.P. Johnson, A.L. Allcock, and P.A. Prodöhl. The project was carried out in partnership with researchers from University College Dublin and University College Cork. We would like to thank Olwyen Mulholland, Mark Jessopp, Jenny Leal-Flórez, Maria Hughes and Julia Nunn for assistance with fieldwork and helpful discussions.
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McInerney, C.E., Allcock, A.L., Johnson, M.P. et al. Characterization of polymorphic microsatellites for the periwinkle gastropod, Littorina littorea (Linnaeus, 1758) and their cross-amplification in four congeners. Conserv Genet 10, 1417–1420 (2009). https://doi.org/10.1007/s10592-008-9750-7
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DOI: https://doi.org/10.1007/s10592-008-9750-7