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Isolation and characterization of 11 microsatellite markers for the large yellow croaker, Pseudosciaena crocea

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Abstract

A new set of 11 microsatellite markers was isolated and characterized in the large yellow croaker (Pseudosciaena crocea Richardson) from a CA-enriched genomic library. Of 35 microsatellite markers screened, 11 microsatellite markers are highly polymorphic in one hatchery stock which contained 58 individuals. The high genetic variability was represented mainly by the number of alleles, which ranged from 7 to 16, and the observed heterozygosity, which ranged from 0.43 to 0.79. These newly isolated markers increase the available molecular resources that can be used to analyze genetic diversity and population structure of large yellow croaker.

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Acknowledgements

This study was supported by the National High Technology Research and Development Program of China under contract No. 2006AA10A405.

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Authors and Affiliations

  1. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Harbin, 150070, China

    Y. M. Chang & L. Q. Liang

  2. Life Science of Technology Institute, Dalian Fisheries University, Dalian, 116023, China

    L. Ding

  3. Department of Life Science and Engineering, Harbin Institute of Technology, Harbin, 150001, China

    W. W. Wang

  4. College of Life Sciences, Zhongshan University, Guangzhou, 510275, China

    J. G. He

  5. College of Electrical & Electronic Engineering, Harbin University of Science and Technology, Harbin, 150080, China

    Q. Q. Lei

Authors
  1. Y. M. Chang
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  2. L. Ding
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  3. W. W. Wang
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  4. J. G. He
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  5. L. Q. Liang
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  6. Q. Q. Lei
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Correspondence to L. Q. Liang.

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Chang, Y.M., Ding, L., Wang, W.W. et al. Isolation and characterization of 11 microsatellite markers for the large yellow croaker, Pseudosciaena crocea . Conserv Genet 10, 1405–1408 (2009). https://doi.org/10.1007/s10592-008-9708-9

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  • DOI: https://doi.org/10.1007/s10592-008-9708-9

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