Abstract
Nine microsatellite loci were isolated and characterized from the clonal salt-marsh sedge Carex scabrifolia, and genetic diversities within four populations were analyzed. The number of alleles per locus ranged from 2 to 7, with an average of 4.7. The observed and expected heterozygosities ranged from 0.000 to 1.000 and 0.000 to 0.679, respectively. In two populations, almost all polymorphic loci showed a significant excess of heterozygotes due to their high clonal reproduction and low number of genotypes (genets) composing the populations. Thus, the number of identical genets varied greatly among populations and ranged from 1 to 28, irrespective of population size (i.e., population area and ramet number). When attempting to conserve and restore clonal plants such as C. scabrifolia, it is important to preserve sufficient genetic diversity within a population, which can be assessed using genetic markers such as the simple sequence repeat markers described here.
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References
Frey JE, Frey B, Sauer C, Kellerhals M (2004) Efficient low-cost DNA extraction and multiplex fluorescent PCR method for marker-assisted selection in breeding. Plant Breed 123:554–557. doi:10.1111/j.1439-0523.2004.01033.x
Ishikawa SI, Kachi N (1998) Shoot population dynamics of Carex kobomugi on a coastal sand dune in relation to its zonal distribution. Aust J Bot 46:111–121. doi:10.1071/BT96105
Kalinowski ST, Taper ML, Marshall TC (2007) Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol 16:1099–1106. doi:10.1111/j.1365-294X.2007.03089.x
Katsuyama T (2005) Carex of Japan (in Japanese). Bun-Ichi Sogo Syuppann, Tokyo
Kyoto Prefecture (2002) Red data book of Kyoto Prefecture. Vol. 1. Wildlife (plants and animals). Kyoto Prefecture, Kyoto
Lian C, Hogetsu T (2002) Development of microsatellite markers in black locust (Robinia pseudoacacia) using dual-suppression-PCR technique. Mol Ecol Notes 2:211–213. doi:10.1046/j.1471-8286.2002.00213.x
Lian CL, Wadud MA, Geng QF, Shimatani K, Hogetsu T (2006) An improved technique for isolating codominant compound microsatellite markers. J Plant Res 119:415–417. doi:10.1007/s10265-006-0274-2
Ohbayashi K, Hodoki Y, Nakayama S, Shimada M, Kunii H (in press) Development of new microsatellite markers from a salt-marsh sedge Carex rugulosa by compound simple sequence repeat–polymerase chain reaction. Mol Ecol Res. doi:10.1111/j.1755-0998.2008.02212.x
Palop-Esteban M, Segarra-Moragues JG, Gonzalez-Candelas F (2007) Historical and biological determinants of genetic diversity in the highly endemic triploid sea lavender Limonium dufourii (Plumbaginaceae). Mol Ecol 16:3814–3827
Rasmussen KK, Kollmann J (2008) Low genetic diversity in small peripheral populations of a rare European tree (Sorbus torminalis) dominated by clonal reproduction. Conserv Genet. doi:10.1007/s10592-007-9492-y
Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225
Rousset F (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Eol Res 8:103–106
Acknowledgments
This study was supported by grants from the Japan Society for the Promotion of Science (Grant-in-Aid for Scientific Research (A) (19201017)) and Foundation for Riverfront Improvement and Restoration.
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Hodoki, Y., Ohbayashi, K. & Kunii, H. Genetic analysis of salt-marsh sedge Carex scabrifolia Steud. populations using newly developed microsatellite markers. Conserv Genet 10, 1361–1364 (2009). https://doi.org/10.1007/s10592-008-9678-y
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DOI: https://doi.org/10.1007/s10592-008-9678-y