Abstract
The present study reports the isolation and characterization of eight new polymorphic microsatellite loci from the sexually deceptive orchid Ophrys fusca. Microsatellites were isolated from two partially enriched genomic libraries using FIASCO (Fast Isolation by AFLP of Sequences COntaining repeats). Seventy-three loci were screened for primer design and primer pairs corresponding to eight different loci were selected for microsatellite characterization of two Portuguese populations. Total number of alleles per locus ranged from 10 to 32. All loci showed a high level of observed heterozygosity (H0) ranging from 0.33 to 1 and were possible to amplify in 16 other species of Ophrys using the same primers.
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Acknowledgements
This work was financed by the Portuguese Foundation of Science and Technology (FCT) with the project POCTI/BSE/48790/2002 and grants SFRH/BPD/5738/2001 and SFRH/BPD/22447/2005.
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H. C. Cotrim and F. A. Monteiro have contributed equally to this work.
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Cotrim, H.C., Monteiro, F.A., Sousa, E.S. et al. Isolation and characterization of novel polymorphic nuclear microsatellite markers from Ophrys fusca (Orchidaceae) and cross-species amplification. Conserv Genet 10, 739–742 (2009). https://doi.org/10.1007/s10592-008-9634-x
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DOI: https://doi.org/10.1007/s10592-008-9634-x