Abstract
Noble crayfish (Astacus astacus) is listed as vulnerable by the IUCN Red List of Threatened Species in Europe. However, very little is known about genetic diversity and structuring of noble crayfish populations, mainly because of the lack of informative genetic markers. We describe the isolation and characterization of the first microsatellite markers for this species, which were obtained by screening 4,000 recombinant clones. Eight loci revealed polymorphisms in a panel of 172 individuals from seven populations in Northern Europe. Number of alleles per locus ranged from two to 10 (average 4.4) and heterozygosity levels among populations varied from 0 to 0.80 for H o and from 0 to 0.72 for H e.
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Aknowledgements
The study was financed by grants no. 6155 and 5729 from the Estonian Science Foundation. The authors express sincere thanks to Margo Hurt, Anna Alaranta and Trude Vrålstad for providing crayfish samples and to Bernhard Gum, Marin Laube and Jürgen Geist for their kind help in laboratory.
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Kõiv, K., Gross, R., Paaver, T. et al. Isolation and characterization of first microsatellite markers for the noble crayfish, Astacus astacus . Conserv Genet 9, 1703–1706 (2008). https://doi.org/10.1007/s10592-008-9539-8
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DOI: https://doi.org/10.1007/s10592-008-9539-8