Abstract
A total of 13 polymorphic microsatellite markers were developed for Atlantic cod (Gadus morhua L.) from a repeat-enriched library. Polymorphism of each locus was assessed in 96 unrelated individuals from a natural population. The number of alleles per locus varied from 8 to 45. The ranges of observed and expected heterozygosity were 0.122–0.907 and 0.673–0.965, respectively. Four of the loci (Gmo-G24, Gmo-G40, Gmo-G46 and Gmo-G49) followed Hardy–Weinberg expectation. No evidence for linkage disequilibrium between pairs of loci was found in any combination of loci pairs, except between Gmo-G40 and Gmo-G43. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and for constructing linkage maps of Atlantic cod.
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Acknowledgements
The present study was supported by the National Programme for Research in Functional Genomics in Norway (FUGE) within the Research Council of Norway (no. 162486/S10), by the Research Council of Norway (no. 164981/S40) and by the EU project SEAFOODplus (no. 506359). We thank Dr. Marie Cooper for reading of the manuscript and making helpful suggestions.
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Jon-Ivar Westgaard and Tekle Tafese have contributed equally to the work.
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Westgaard, JI., Tafese, T., Wesmajervi, M.S. et al. Identification and characterisation of thirteen new microsatellites for Atlantic cod (Gadus morhua L.) from a repeat-enriched library. Conserv Genet 8, 749–751 (2007). https://doi.org/10.1007/s10592-006-9200-3
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DOI: https://doi.org/10.1007/s10592-006-9200-3