Abstract
Although the Louisiana black bear (Ursus americanus luteolus) is currently listed as threatened under the Endangered Species Act, there have been no attempts to estimate range-wide abundance. This subspecies was thought to occupy a near contiguous range across southern Mississippi, Louisiana and east Texas but is now restricted to three isolated areas in Louisiana. In 1964, Louisiana initiated a restocking program in which black bears from Minnesota were introduced into two of these areas. It is not clear how the additions affected population structure or if substantial breeding occurred between native and introduced bears. Using baited sites to snare hair samples, and microsatellite DNA analysis to distinguish individuals, we estimated abundance of two geographically isolated bear populations in south central Louisiana: Inland and Coastal. Additionally, we examined genetic variation both within and between the two populations. Mark recapture analysis of the distribution of individual captures during two primary sampling periods resulted in population estimates of 77 ± 9 for Coastal and 41 ± 6 for Inland. Genetic analysis revealed significant population differentiation (F ST = 0.206) between the two populations. The apparently smaller Inland population exhibited more diversity than the Coastal, which suggests that the genetic structure of the Inland population has been influenced by the reintroduction. Both of these populations are isolated and face considerable demographic and genetic threats, thus conservation measures to protect both are warranted. However, the Coastal population is more representative of Louisiana black bears prior to reintroduction and special consideration should be given to insure its integrity.
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References
FW Allendorf (1986) ArticleTitleGenetic drift and the loss of alleles versus heterozygosity Zoo Bio. 5 181–190
Leary Allendorf FW RF Leary (1986) Heterozygosity and fitness in natural populations of animals. In: The Natural History of Inbreeding and Outbreeding (ed. Thornhill NW) The University of Chicago Press Chicago 57–76
M Boersen Clark JD King TL (2003) ArticleTitleEstimating black bear population density and genetic diversity at Tensas River Louisiana using microsatellite DNA markers. Wildl. Soc. Bull. 31 197–207
CM Clarke D Immell SK Wasser (2001) ArticleTitleTechnical considerations for hair genotyping methods in black bears West. Workshop Black Bear Res. Manage. 7 24–29
PL Clarkson IS Liepins (1994) ArticleTitleGrizzly bear population estimate and characteristics in the Anderson and Horton Rivers Area, Northwest Territories 1987–1989. Int. Conf. Bear Res. and Manage. 9 213–221
WG Cochran (1977) Sampling Techniques EditionNumber3rd edition. John Wiley and Sons Inc., New York
Cornuet J-M Luikart G (1996) ArticleTitleDescription and power analysis of two tests for detecting recent population bottlenecks from allele frequency data Genetics 144 2001–2014
J Craighead D Paetkau HV Reynolds ER Vyse C Strobeck (1995) ArticleTitleMicrosatellite analysis of paternity and reproduction in Arctic grizzly bears J. Heredity 86 255–261
M Cronin J Shideler J Hechtel C Strobeck D Paetkau (1999) ArticleTitleGenetic relationships of grizzly bears (Ursus arctos) in the Prudhoe Bay Region of Alaska: Inference from microsatellite DNA, mitochondrial DNA and field observations. J.␣Heredity 90 622–628
I Csiki Lam C Key A Coulter E Clark JD Pace III RM Smith KG Rhoads DD (2003) ArticleTitleGenetic variation in black bears in Arkansas and Louisiana using microsatellite DNA markers J. Mammal. 84 691–701
AE Derocher I Stirling (1995) ArticleTitleEstimation of polar bear population size and survival in western Hudson Bay J. Wildl. Manage. 59 215–221
P Donnelly (1995) ArticleTitleNonindependence of matches at different loci in DNA profiles: Quantifying the effect of close relatives on the match probability Heredity 75 26–34
Environmental Systems Research Institute (1996–1998) ArcView GIS (Version 3.1). Environmental Systems Research Institute, Redlands, California.
DJ Finney (1948) ArticleTitleThe Fisher-Yates test of significance in 2 × 2 contingency tables Biometrika 35 145–156
Foran S Minta C Heinemeyer KS (1997) ArticleTitleDNA-based analysis of hair to identify species and individuals for population research and monitoring Wildl. Soc. Bul. 25 840–847
R Frankham (1995) ArticleTitleInbreeding and extinction: A threshold effect Conserv. Biol. 99 792–799
P Gagneux C Boesch DS Woodruff (1997) ArticleTitleMicrosatellite scoring errors associated with noninvasive genotyping based on nuclear DNA amplified from shed hair Mol. Ecol. 6 861–868
DL Garshelis LG Visser (1997) ArticleTitleEnumerating megapopulations of wild bears with an ingested biomarker J. Wildl. Manage. 59 215–221
B Goossens LP Waits P Taberlet (1998) ArticleTitlePlucked hair samples as a source of DNA: Reliability of dinucleotide microsatellite genotyping Mol. Ecol. 7 1237–1241
Goudet J (2000) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.1). Updated from Goudet 1995.
SM Haig (1998) ArticleTitleMolecular contributions to conservation Ecology 79 413–426
S Hartley RM III Pace JB Johnston M Swan CP O’Neal L Handley L Smith (2000) A Gap analysis of Louisiana. p.␣558. US Geological Survey Lafayette LA
EC Hellgren MR Vaughn (1989) ArticleTitleDemographic analysis of a black bear population in the Great Dismal Swamp J. Wildl. Manage. 53 969–977
CR Hughes DC Queller (1993) ArticleTitleDetection of highly polymorphic microsatellite loci in a species with little allozyme polymorphism Mol. Ecol. 2 131–137
LF Keller DM Waller (2002) ArticleTitleInbreeding effects in wild populations Trends. Ecol. Evol. 17 230–241
E Kitahara Y Isagi Y Ishabashis T Saitoh (2000) ArticleTitlePolymorphic microsatellite DNA markers in the Asiatic black bear Ursus thibetanus Mol. Ecol. 9 1661–1686
M Kohn F Knauer A Stoffela W Schroder S Pääbo (1995) ArticleTitleConservation genetics of the European brown bear-a study using excremental PCR of nuclear and mitochondrial sequences Mol. Eco. 4 95–103
GH Lowery (1974) The mammals of Louisiana and its adjacent waters. Louisiana State University Press Baton Rouge Louisiana
Luikart G Cornuet J-M Allendorf FW (1999) ArticleTitleTemporal changes in allele frequencies provide estimates of population bottleneck size Cons. Biol. 13 523–530
NJ Lunn D Paetkau W Calvert S Atkinson M Taylor C Strobeck (2000) ArticleTitleCub adoption by polar bears (Ursus maritimus): Determining relatedness with microsatellite markers J. Zool. Lond. 251 23–30
RD Mace SC Minta TL Manley KE Aune (1994) ArticleTitleEstimating grizzly bear population size using camera sightings Wildl. Soc. Bull. 22 74–83
DS Maehr (1984) ArticleTitleDistribution of black bears in eastern North America East Workshop Black Bear Res. and Manage. 7 74–76
GW McDaniel KS McKelvey JR Squires LF Ruggiero (2000) ArticleTitleEfficacy of lures and hair snares to detect lynx Wildl. Soc. Bull. 28 119–123
CH Merriam (1893) ArticleTitleThe yellow bear of Louisiana Ursus luteolus Griffith. Proc. Biol. Soc.Washington. 8 147–152
DA Miller EM Hallerman MR Vaughn JW Kasbohm (1998) ArticleTitleGenetic variation in black bear populations from Louisiana and Arkansas: Examining the potential influence of reintroductions from Minnesota Ursus 10 335–341
LS Mills JJ Citta KP Lair MK Schwartz DA Tallmon (2000) ArticleTitleEstimating animal abundance using noninvasive DNA sampling: Promise and pitfalls. Ecol. Appl. 10 283–294
JB Mitton (1993) Theory and data pertinent to relationship between heterozygosity and fitness. In: Conservation Biology: the Science of Scarcity and Diversity (ed. Soule ME) Sinauer Associates Sunderland, Massachusetts 57–76
G Mowat C Strobeck (2000) ArticleTitleEstimating population size of␣grizzly bears using hair capture, DNA profiling and mark-recapture analysis. J. Wildl. Manage. 64 183–193
WA Neal (1992) ArticleTitleListing the Louisiana black bear as a threatened species and designation of other free-living bears of the species U americanus as threatened due to similarity of appearance. Federal Register 58 588–595
M Nei T Maruyama R Chakraborty (1975) ArticleTitleThe bottleneck effect and genetic variability in populations Evolution 29 1–10
RM Nowak (1986) Status of the Louisiana black bear. Office of␣Endangered Species US Fish and Wildlife Service Washington D.C
DL Otis KP Burnham GC White DR Anderson (1978) ArticleTitleStatistical inference from capture data on closed animal populations Wildl. Monogr. 62 1–135
RM III Pace DR Anderson S Shively (2000) ArticleTitleSources and patterns of mortality in Louisiana black bears Southeastern Assoc. Fish Wildl. Agencies. 54 365–373
D Paetkau (2003) ArticleTitleAn empirical exploration of data quality in␣DNA-based population inventories Mol. Ecol. 12 1375–1387
D Paetkau W Calvert I Stirling C Strobeck (1995) ArticleTitleMicrosatellite analysis of population structure in Canadian polar bears Mol. Ecol. 4 347–354
D Paetkau GF Shields C Strobeck (1998) ArticleTitleGene flow between insular coastal and interior populations of brown bears in Alaska. Mol. Ecol. 7 1283–1292
D Paetkau C Strobeck (1994) ArticleTitleMicrosatellite analysis of genetic variation in black bear populations Mol. Ecol. 3 489–495
D Paetkau C Strobeck (1995) ArticleTitleThe molecular basis and evolutionary history of a microsatellite null allele in bears Mol. Ecol. 4 519–520
D Paetkau LP Waits PL Clarkson L Craighead C Strobeck (1997) ArticleTitleAn empirical valuation of genetic distance statistics using microsatellite data from bear (Ursidae) populations Genetics 147 1943–1957
PJ Palsb\’\oll J Allen M Berube PJ Clapham TP Feddersen PS Hammond RR Hudson H Jorgensen S Katona AH Larsen F Larsen J Lien DK Mattila J Sigurjonsson R Sears T Smith R Sponer P Stevick N Oien (1997) ArticleTitleGenetic tagging of humpback whales Nature 388 767–769
PG Parker AA Snow MD Schug GC Booton PA Fuerst (1998) ArticleTitleWhat molecules can tell us about a population: Choosing and using a molecular marker Ecology 79 361–382
MR. Pelton, 1982, Black bear. In: Wild Mammals of North America eds. Chapman JA Feldhamer GA, pp. 504–514. The John Hopkins University Press, Baltimore, Maryland
MR Pelton (1989) The Louisiana Black Bear: Status and Future. Special report to the US Fish and Wildlife Service Jackson Mississippi
MR Pelton (1990) ArticleTitleBlack bears in the southeast: To list or not␣to list East Workshop Black Bear Res. Manage. 10 155–161
KH Pollock JD Nichols C Brownie JE Hines (1990) ArticleTitleStatistical-inference for capture-recapture experiments Wildl. Monogr. 107 1–97
JK Pritchard M Stephens P Donnelly (2000) ArticleTitleInference of population structure using multilocus genotype data Genetics 155 945–959
DC Queller JE Strassman CR Hughes (1993) ArticleTitleMicrosatellites and kinship Trends Ecol. Evol. 8 285–288
M Raymond F Rousset (1995) ArticleTitleGENEPOP (version 1.2): Population genetics software for exact tests and ecumenicism J. Heredity 86 248–249
SAS Institute Inc (1990) SAS User’s Guide: version 6.12. SAS Institute Inc. Cary North Carolina
A Schenk KM Kovacs (1996) ArticleTitleGenetic variation in a population of black bears as revealed by DNA fingerprinting J. Mammal. 77 942–950
A Schenk ME Obbard KM. Kovacs (1998) ArticleTitleGenetic relatedness and home-range overlap among female black bears (Ursus americanus) in northern Ontario, Canada Can. J. Zool. 76 1511–1519
GAF Seber (1982) The estimation of animal abundance and related parameters 2nd edition Charles Griffin London
TR Smith MR Pelton (1990) ArticleTitleHome ranges and movements of black bears in a bottomland hardwood forest in Arkansas Int. Conf. Bear Res. Manage. 8 213–218
P Taberlet J Bouvet (1992) Bear conservation genetics. Nature 358 197
P Taberlet JJ Camarra GE Uhres O Hannotte LP Waits C Dubois-Paganon CT Burke J Bouvet (1997) ArticleTitleNoninvasive genetic tracking of the endangered Pyrenean brown bear population Mol. Ecol. 6 869–876
P Taberlet S Griffin B Goossens S Questiau V Manceau N Escaravage LP Waits J Bouvet (1996) ArticleTitleReliable genotyping of samples with very low DNA quantities using PCR Nucl. Acids Res. 24 3189–3194
P Taberlet G Luikart (1999) ArticleTitleNon-invasive genetic sampling and individual identification Biol. J. Linn. Soc. 68 41–55
P Taberlet H Mattock C Dubois-Paganon J Bouvet (1993) ArticleTitleSexing free-ranging brown bears Ursus arctos using hairs found in the field Mol. Ecol. 2 399–403
DF Taylor (1971) A Radio-Telemetry Study of the Black Bear (Euarctos americanus) with Notes on its History and Present Status in Louisiana. MS Thesis Louisiana State University Baton Rouge, Louisiana
DA Triant (2001) Estimating Population Size and Genetic Diversity of Two Populations of Black Bears in South Central Louisiana. MS Thesis Louisiana State University Baton Rouge, Louisiana
US Fish Wildlife Service (1995) Louisiana Black Bear Recovery Plan Jackson Mississippi
AJ van Noordwijk (1994) The interaction of inbreeding depression and environmental stochasticity in the risk of extinction of small populations. V Loeschcke J Tomiuk SK Jain (Eds) Conservation Genetics Birkhauser Verlag Basel, Switzerland 131–146
HW Wagner KM Sefc (1999) Identity 1.0. Center for Applied Genetics University of Agricultural Sciences Vienna, Austria
RO Wagner (1995) Movement Patterns of Black Bears in South Central Louisiana. MS Thesis Louisiana State University Baton Rouge, Louisiana
JL Waits PL Leberg (2000) ArticleTitleBiases associated with population estimation using molecular tagging Anim. Conserv. 3 191–199
J Warrillow M Culver E Hallerman M Vaughn (2001) ArticleTitleSubspecific affinity of black bears in the White River National Wildlife Refuge J. Heredity 92 226–233
GC White DR Anderson KP Burnham DL Otis (1982) Capture-recapture and removal methods for sampling closed populations. Los Alamos National Laboratory LA-8787-NERP Los Alamos, New Mexico
JB Wooding JR Cox MR Pelton (1994) ArticleTitleDistribution of black bears in the southeastern coastal plain Proc. Annu. Conf. of the Southeast. Assoc. of Fish and Wildl. Agencies. 48 270–275
JG Woods D Paetkau D Lewis BN McLellan M Proctor C Strobeck (1999) ArticleTitleGenetic tagging of free-ranging black and brown bears Wildl. Soc. Bull. 27 616–627
S Wright (1943) ArticleTitleIsolation by distance Genetics 28 114–138
S Wright (1965) ArticleTitleThe interpretation of population structure by F-statistics with special regard to systems of mating Evolution 19 395–420
Wu R Zeng Z-B (2001) ArticleTitleJoint linkage and linkage disequilibrium mapping in natural populations Genetics 157 899–909
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Triant, D.A., Pace, R.M. & Stine, M. Abundance, genetic diversity and conservation of Louisiana black bears (Ursus americanus luteolus) as detected through noninvasive sampling. Conserv Genet 5, 647–659 (2004). https://doi.org/10.1007/s10592-004-1861-1
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DOI: https://doi.org/10.1007/s10592-004-1861-1