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Identification and diversity of functional centromere satellites in the wild rice species Oryza brachyantha

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Abstract

The centromere is a key chromosomal component for sister chromatid cohesion and is the site for kinetochore assembly and spindle fiber attachment, allowing each sister chromatid to faithfully segregate to each daughter cell during cell division. It is not clear what types of sequences act as functional centromeres and how centromere sequences are organized in Oryza brachyantha, an FF genome species. In this study, we found that the three classes of centromere-specific CentO-F satellites (CentO-F1, CentO-F2, and CentO-F3) in O. brachyantha share no homology with the CentO satellites in Oryza sativa. The three classes of CentO-F satellites are all located within the chromosomal regions to which the spindle fibers attach and are characterized by megabase tandem arrays that are flanked by centromere-specific retrotransposons, CRR-F, in the O. brachyantha centromeres. Although these CentO-F satellites are quantitatively variable among 12 O. brachyantha centromeres, immunostaining with an antibody specific to CENH3 indicates that they are colocated with CENH3 in functional centromere regions. Our results demonstrate that the three classes of CentO-F satellites may be the major components of functional centromeres in O. brachyantha.

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Abbreviations

BAC:

bacterial artificial chromosome

CentO-F:

centromere-specific satellite repeat of FF genome

CRR-F:

centromere-specific retrotransposon of FF genome

FISH:

fluorescence in-situ hybridization

CEN:

centromere of chromosome

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Acknowledgments

This work was supported by grants from the National Basic Research Program of China (“973” Program, 2010CB125904, 2013CBA01405), the National Natural Science Foundation of China (31071382, 30771210) and the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).

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Authors and Affiliations

  1. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of Ministry of Education, College of Agriculture, Yangzhou University, Yangzhou, 225009, China

    Chuandeng Yi, Xibin Dai, Xing Li, Zhiyun Gong, Yong Zhou, Guohua Liang & Minghong Gu

  2. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China

    Wenli Zhang

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  1. Chuandeng Yi
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  2. Wenli Zhang
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  3. Xibin Dai
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  4. Xing Li
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  5. Zhiyun Gong
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  6. Yong Zhou
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  7. Guohua Liang
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  8. Minghong Gu
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Correspondence to Chuandeng Yi or Minghong Gu.

Additional information

Responsible Editor: Hans de Jong.

Data deposition: The sequences reported in this paper have been deposited in the GenBank database (accession nos. KF293389-KF293391)

Chuandeng Yi and Wenli Zhang contributed equally to this work.

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Yi, C., Zhang, W., Dai, X. et al. Identification and diversity of functional centromere satellites in the wild rice species Oryza brachyantha. Chromosome Res 21, 725–737 (2013). https://doi.org/10.1007/s10577-013-9374-8

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