Abstract
The genetic classification for the N-genome chromosomes has been developed on the basis of C-banding analysis on the set of Triticum aestivum × Aegilops uniaristata single chromosome addition lines and examination of A. uniaristata (\( {\text{2n = 2}} \times { = 14} \), NN), Aegilops ventricosa (\( {\text{2n = 4}} \times { = 28} \), DDNN) and Aegilops recta (\( {\text{2n = 6}} \times { = 42} \), UUXnXnNN) accessions carrying intergenomic translocations using fluorescence in situ hybridisation with probes for three repetitive DNA sequences as well as the 5S and 45S rDNA families. The N-genome chromosomes of the tetraploid A. ventricosa show significant changes relative to the diploid progenitor species, while those of the hexaploid A. recta are similar to A. uniaristata with regard to the distribution of C-bands, 45S and 5S rDNA loci and hybridisation sites of all the three families of tandem repeats. The possible mechanisms of N-genome evolution are discussed.
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Abbreviations
- FISH:
-
Fluorescence in situ hybridisation
- NORs:
-
Nucleolar organising regions
- RFLP:
-
Restriction fragment length polymorphism
- PCR:
-
Polymerase chain reaction
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Acknowledgements
The authors thank Dr. N.N. Chikida (N.I. Vavilov Institute of Plant Industry VIR, St-Petersburg, Russia), Dr. H. Bockelman (Small Grain Collection, Aberdeen, Idaho, USA) and Dr. M. Feldman (Weizmann Institute of Science, Rehovot, Israel) for providing the materials for this study. This work was supported by a grant from the Russian State Foundation for Basic Research (project nos. 11-04-00126 and 11-04-00214).
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Badaeva, E.D., Dedkova, O.S., Zoshchuk, S.A. et al. Comparative analysis of the N-genome in diploid and polyploid Aegilops species. Chromosome Res 19, 541–548 (2011). https://doi.org/10.1007/s10577-011-9211-x
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DOI: https://doi.org/10.1007/s10577-011-9211-x