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Chromosome elimination by wide hybridization between Triticeae or oat plant and pearl millet: pearl millet chromosome dynamics in hybrid embryo cells

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Abstract

Wide crossing is one of a number of practical methods that can be used to expand genetic variation in common wheat (Triticum aestivum). However, in crosses between wheat and distantly related species such as maize (Zea mays) and pearl millet (Pennisetum glaucum), non-wheat chromosomes are often eliminated from the hybrid during embryogenesis. In this study, we used pearl millet pollen to pollinate the pistils of a range of plants in the tribe Triticeae, as well as oat. Seven days after pollination, the dynamics of the pearl millet chromosomes in the embryos were observed using in situ hybridization, probing both the pearl millet genomic DNA and its centromere-specific repeats. In embryos from the crosses with oat, all seven of the pearl millet chromosomes were retained. However, in hybrids with the Triticeae species, chromosome elimination occurred during embryogenesis. Pearl millet chromosome showed chromosome rearrangements and non-disjunction together with micronuclei. These rearranged chromosomes and micronuclei derived from the breakage of bridges and retention of acentric fragments in anaphase, respectively. The cause of the chromosome elimination of wheat–pearl millet hybrid is not malfunction of the kinetochores binding to the spindles but the malfunction of the sister chromatids segregation at anaphase especially of chromosome arm.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxy acetic acid

GISH:

Genomic in situ hybridization

FISH:

Fluorescence in situ hybridization

DAPI:

4′,6-Diamidino-2-phenylindole

References

  • Barclay IR (1975) High frequencies of haploid production in wheat (Triticum aestivum) by chromosome elimination. Nature 256:410–411

    Article  Google Scholar 

  • Bennett MD (1984) Nuclear architecture and its manipulation. In: Gustafson JP (ed) Gene manipulation in plant improvement. Plenum, New York, pp 469–502

    Google Scholar 

  • Elamein HMM, Ali AM, Garg M, Kikuchi S, Tanaka H, Tsujimoto H (2007) Evolution of chromosomes in genus Pennisetum. Chromosome Sci 10:55–63

    CAS  Google Scholar 

  • Gernand D, Rutten T, Varshney A, Rubtsova M, Prodanovic S, Brub C, Kumlehn J, Houben A (2005) Uniparental chromosome elimination at mitosis and interphase in wheat and pearl millet crosses involves micronucleus formation, progressive heterochromatinization, and DNA fragmentation. Plant Cell 17:2431–2438

    Article  CAS  PubMed  Google Scholar 

  • Gernand D, Rutten T, Pickering R, Houben A (2006) Elimination of chromosomes in Hordeum vulgare × H. bulbosum crosses at mitosis and interphase involves micronucleus formation and progressive heterochromatinization. Cytogenet Genome Res 114:169–174

    Article  CAS  PubMed  Google Scholar 

  • Inagaki MN, Mujeeb-Kazi A (1995) Comparison of polyhaploid production frequencies in crosses of hexaploid wheat with maize, pearl millet and sorghum. Breeding Sci 45:157–161

    Google Scholar 

  • Kamm A, Schmidt T, Heslop-Harrison JS (1994) Molecular and physical organization of highly repetitive, undermethylated DNA from Pennisetum glaucum. Mol Gen Genet 244:420–425

    Article  CAS  PubMed  Google Scholar 

  • Kasha KJ, Kao KN (1970) High frequency haploid production in barley (Hordeum vulgare L.). Nature 225:874–876

    Article  CAS  PubMed  Google Scholar 

  • Komeda N, Chaudhary HK, Suzuki G, Mukai Y (2007) Cytological evidence for chromosome elimination in wheat × Imperata cylindrica hybrids. Genes Genet Syst 82:241–248

    Article  PubMed  Google Scholar 

  • Laurie DA (1989) The frequency of fertilization in wheat × pearl millet crosses. Genome 32:1063–1067

    Google Scholar 

  • Laurie DA, Bennett MD (1986) Wheat × maize hybridization. Can J Genet Cytol 28:313–316

    Google Scholar 

  • Laurie DA, Bennett MD (1988) Cytological evidence for fertilization in hexaploid wheat × sorghum crosses. Plant Breed 100:73–82

    Article  Google Scholar 

  • Laurie DA, Bennett MD (1989) The timing of chromosome elimination in hexaploid wheat × maize crosses. Genome 32:953–961

    Google Scholar 

  • Leitch AR, Schwarzacher T, Mosgöller W, Bennett MD, Heslop-Harrison JS (1991) Parental genomes are separated throughout cell cycle in a plant hybrid. Chromosoma 101:206–213

    Article  CAS  Google Scholar 

  • Liu Z, Makaroff CA (2006) Arabidopsis separase AESP is essential for embryo development and the release of cohesin during meiosis. Plant Cell 18:1213–1225

    Article  CAS  PubMed  Google Scholar 

  • Mochida K, Tsujimoto H (2001) Production of wheat double haploids by pollination with Job’s Tears (Coix lacryma-jobi L.). Heredity 92:81–83

    Article  CAS  Google Scholar 

  • Mochida K, Tsujimoto H, Sasakuma T (2004) Confocal analysis of chromosome behavior in wheat × maize zygotes. Genome 47:199–205

    Article  PubMed  Google Scholar 

  • Okagaki RJ, Kynast RG, Livingston SM, Russell CD, Rines HW, Phillips RL (2001) Mapping maize sequences to chromosomes using oat–maize chromosome addition materials. Plant Physiol 125:1228–1235

    Article  CAS  PubMed  Google Scholar 

  • Riera-Lizarazu O, Rines HW, Phillips RL (1996) Cytological and molecular characterization of oat × maize partial hybrids. Theor Appl Genet 93:123–135

    Article  CAS  Google Scholar 

  • Sanei M, Pickering R, Fuchs J, Banaei Moghaddam AM, Dziurlikowska A, Houben A (2010) Interspecific hybrids of Hordeum marinum ssp. marinum × H. bulbosum are mitotically stable and reveal no gross alterations in chromatin properties. Cytogenet Genome Res 129:110–116

    Article  CAS  PubMed  Google Scholar 

  • Schwarzacher-Robinson T, Finch RA, Smith JB, Bennett MD (1987) Genotypic control of centromere positions of parental genomes in Hordeum × Secale hybrid metaphases. J Cell Sci 87:291–304

    Google Scholar 

  • Schwarzacher T, Leitch AR, Bennett MD, Heslop-Harrison JS (1989) In situ localization of parental genomes in a wide hybrid. Ann Bot 64:315–324

    Google Scholar 

  • Skibbens RV (2000) Holding your own: establishing sister chromatid cohesion. Genome Res 10:1664–1671

    Article  CAS  PubMed  Google Scholar 

  • Uhlmann F (2001) Secured cutting: controlling separase at the metaphase to anaphase transition. EMBO Rep 2:487–492

    CAS  PubMed  Google Scholar 

  • Uhlmann F (2003) Chromosome cohesion and separation: from men and molecules. Curr Biol 13:R104–R114

    Article  CAS  PubMed  Google Scholar 

  • Waizenegger IC, Hauf S, Meinke A, Peters JM (2000) Two distinct pathways remove mammalian cohesin from chromosome arms in prophase and from centromeres in anaphase. Cell 103:339–410

    Article  Google Scholar 

  • Zenkteler M, Straub J (1979) Cytoembryogical studies on the process of fertilization and the development of haploid embryos of Triticum aestivum L. (2n = 42) after crossing with Hordeum bulbosum (2n = 14). Z Pflanzenzüchtg 82:36–44

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Plant Genetics and Breeding Science, Graduate School of Agriculture, Tottori University, Tottori, Japan

    Takayoshi Ishii, Toshie Ueda, Hiroyuki Tanaka & Hisashi Tsujimoto

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  1. Takayoshi Ishii
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  2. Toshie Ueda
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  3. Hiroyuki Tanaka
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  4. Hisashi Tsujimoto
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Correspondence to Hisashi Tsujimoto.

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Responsible Editor: Pat Heslop-Harrison

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Ishii, T., Ueda, T., Tanaka, H. et al. Chromosome elimination by wide hybridization between Triticeae or oat plant and pearl millet: pearl millet chromosome dynamics in hybrid embryo cells. Chromosome Res 18, 821–831 (2010). https://doi.org/10.1007/s10577-010-9158-3

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  • DOI: https://doi.org/10.1007/s10577-010-9158-3

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