Skip to main content
SpringerLink
Log in

Microdissection and chromosome painting of X and B chromosomes in Locusta migratoria

  • Published:
Chromosome Research Aims and scope Submit manuscript

Abstract

Acquisition of knowledge of the nature and DNA content of B chromosomes has been triggered by a collection of molecular techniques, one of which, microdissection, has provided interesting results in a number of B chromosome systems. Here we provide the first data on the molecular composition of B chromosomes in Locusta migratoria, after microdissection of the B and X chromosomes, DNA amplification by one (B) or two (X) different methods, and chromosome painting. The results showed that B chromosomes share at least two types of repetitive DNA sequences with the A chromosomes, suggesting that Bs in this species most likely arose intraspecifically. One of these repetitive DNAs is located on the heterochromatic distal half of the B chromosome and in the pericentromeric regions of about half of the A chromosomes, including the X. The other type of repetitive DNA is located interspersedly over the non-centromeric euchromatic regions of all A chromosomes and in an interstitial part of the proximal euchromatic half of the B chromosome. Chromosome painting, however, did not provide results sufficiently reliable to determine, in this species, which A chromosome gave rise to the B; this might be done by detailed analysis of the microdissected DNA sequences

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Abbreviations

AFLP:

amplified fragment length polymorphism

CMA3:

chromomycin A3

DAPI:

4′,6-diamidino-2-phenylindole

DOP-PCR:

degenerate oligonucleotide primed PCR

References

  • Brinkman JN, Sessions SK, Houben A, Green DM (2000) Structure and evolution of supernumerary chromosomes in the Pacific giant salamander, Dicamptodon tenebrosus. Chromosome Res 8:477–485

    Article  PubMed  CAS  Google Scholar 

  • Bugrov AG, Karamysheva TV, Pyatkova MS et al (2003) B chromosomes of the Podisma sapporensis Shir. (Orthoptera, Acrididae) analysed by chromosome microdissection and FISH. Folia Biol Krakow 51:1–11

    PubMed  CAS  Google Scholar 

  • Bugrov AG, Karamysheva TV, Rubtsov DN, Andreenkova OV, Rubtsov NB (2004) Comparative FISH analysis of distribution of B chromosome repetitive DNA in A and B chromosomes in two subspecies of Podisma sapporensis (Orthoptera, Acrididae). Cytogenet Genome Res 106:284–288

    Article  PubMed  CAS  Google Scholar 

  • Bugrov AG, Karamysheva TV, Perepelov EA et al (2007) DNA content of the B chromosomes in grasshopper Podisma kanoi Storozh. (Orthoptera, Acrididae). Chromosome Res 15:315–325

    PubMed  CAS  Google Scholar 

  • Cabrero J, Viseras E, Camacho JPM (1984) The B chromosomes of Locusta migratoria.1. Detection of negative correlation between mean chiasma frequency and the rate of accumulation of the Bs. A reanalysis of the available data about the transmission of these B chromosomes. Genetica 64:155–164

    Article  Google Scholar 

  • Cabrero J, Bakkali M, Bugrov A et al (2003) Multiregional origin of B chromosomes in the grasshopper Eyprepocnemis plorans. Chromosoma 112:207–211

    Article  PubMed  CAS  Google Scholar 

  • Cabrero J, Manrique-Poyato MI, Camacho JPM (2006) Detection of B chromosomes in interphase hemolymph nuclei from living specimens of the grasshopper Eyprepocnemis plorans. Cytogenet Genome Res 114:66–69

    Article  PubMed  CAS  Google Scholar 

  • Camacho JPM (2004) B Chromosomes in the Eukaryote Genome. Karger, Basel

    Google Scholar 

  • Camacho JPM (2005) B chromosomes. In: Gregory TR (ed) The evolution of the genome. Elsevier, San Diego, pp 223–286

    Chapter  Google Scholar 

  • Camacho JPM, Cabrero J, Viseras E, López-León MD, Navas-Castillo J, Alché JD (1991) G-Banding in two species of grasshopper and its relationship to C, N, and fluorescence banding techniques. Genome 34:638–643

    Google Scholar 

  • Cheng YM, Lin BY (2003) Cloning and characterization of maize B chromosome sequences derived from microdissection. Genetics 164:299–310

    PubMed  CAS  Google Scholar 

  • Dearn JM (1974) Phase transformation and chiasma frequency variation in locusts. II. Locusta migratoria. Chromosoma 45:339–352

    Article  PubMed  CAS  Google Scholar 

  • Dugan LC, Pattee MS, Williams J et al (2005) Polymerase chain reaction-based suppression of repetitive sequences in whole chromosome painting probes for FISH. Chromosome Res 13:27–32

    Article  PubMed  CAS  Google Scholar 

  • Francki MG (2001) Identification of Bilby, a diverged centromeric Ty1-copia retrotransposon family from cereal rye (Secale cereale L.). Genome 44:266–274

    Article  PubMed  CAS  Google Scholar 

  • Gribble S, Ng BL, Prigmore E, Burford DC, Carter NP (2004) Chromosome paints from single copies of chromosomes. Chromosome Res 12:143–151

    Article  PubMed  CAS  Google Scholar 

  • Hewitt GM (1979) Grasshoppers and crickets. In: Animal Cytogenetics, Vol. 3, Insecta 1. Gebrüder Borntrager, Berlin-Stuttgart

  • Houben A, Kynast RG, Heim U, Hermann H, Jones RN, Forster JW (1996) Molecular cytogenetic characterisation of the terminal heterochromatic segment of the B-chromosome of rye (Secale cereale). Chromosoma 105:97–103

    Article  PubMed  CAS  Google Scholar 

  • Hsiang W (1958) Cytological studies on migratory locust hybrid, Locusta migratoria migratoria L. X Locusta migratoria manilensis Meyen. Acta Zoologica Sinica 10:53–59

    Google Scholar 

  • Itoh H (1934) Chromosomal variation in the spermatogenesis of a grasshopper, Locusta danica L. Jpn J Genet 10:115–134

    Article  Google Scholar 

  • Jamilena M, Garrido-Ramos M, Ruiz-Rejón M, Ruiz-Rejón C, Parker JS (1995) Characterization of repeated sequences from microdissected B chromosomes of Crepis capillaris. Chromosoma 104:113–120

    Article  PubMed  CAS  Google Scholar 

  • Jones RN, Viegas W, Houben A (2008) A century of B chromosomes in plants: So what? Ann Bot 101:767–775

    Article  PubMed  Google Scholar 

  • Karamysheva TV, Andreenkova OV, Bochkaerev MN et al (2002) B chromosomes of Korean field mouse Apodemus peninsulae (Rodentia, Murinae) analysed by microdissection and FISH. Cytogenet Genome Res 96:154–160

    Article  PubMed  CAS  Google Scholar 

  • Kayano H (1971) Accumulation of B chromosomes in the germ line of Locusta migratoria. Heredity 27:119–123

    Article  Google Scholar 

  • King M, John B (1980) Regularities and restrictions governing C-band variation in Acridoid grasshoppers. Chromosoma 76:123–150

    Article  Google Scholar 

  • Lamb J, Kato A, Birchler J (2005) Sequences associated with A chromosome centromeres are present throughout the maize B chromosome. Chromosoma 113:337–349

    Article  PubMed  CAS  Google Scholar 

  • Long H, Qi ZX, Sun XM et al (2008) Characters of DNA constitution in the rye B chromosome. J Integr Plant Biol 50:183–189

    Article  PubMed  CAS  Google Scholar 

  • Lucas JN, Wu X, Guo E, Chi LE, Chen Z (2006) An efficient chemical method to generate repetitive sequences depleted DNA probes. Am J Med Genet Part A 140A:2115–2120

    Article  CAS  Google Scholar 

  • Marchal JA, Acosta MJ, Nietzel H et al (2004) X chromosome painting in Microtus: origin and evolution of the giant sex chromosomes. Chromosome Res 12:767–776

    Article  PubMed  CAS  Google Scholar 

  • McQuade L, Hill R, Francis D (1994) B chromosome systems in the greater glider, Petauroides volans (Marsupialia, Pseudocheiridae). 2. Investigation of B chromosome DNA sequences isolated by micromanipulation and PCR. Cytogenet Cell Genet 66:155–161

    Article  PubMed  CAS  Google Scholar 

  • Nur U (1969) Mitotic instability leading to an accumulation of B chromosomes in grasshoppers. Chromosoma 27:1–19

    Article  PubMed  CAS  Google Scholar 

  • Nur U, Werren J, Eickbush D, Burke W, Eickbush T (1988) A selfish B chromosome that enhances its transmission by eliminating the paternal genome. Science 240:512–514

    Article  PubMed  CAS  Google Scholar 

  • Pardo MC, López-León MD, Cabrero J, Camacho JPM (1994) Transmission analysis of mitotically unstable B chromosomes in Locusta migratoria. Genome 37:1027–1034

    Article  PubMed  CAS  Google Scholar 

  • Pardo MC, López-León MD, Cabrero J, Camacho JPM (1995a) Cytological and developmental analysis of tychoparthenogenesis in Locusta migratoria. Heredity 75:485–494

    Article  Google Scholar 

  • Pardo MC, López-León MD, Viseras E, Cabrero J, Camacho JPM (1995b) Mitotic instability of B chromosomes during embryo development in Locusta migratoria. Heredity 74:164–169

    Article  Google Scholar 

  • Peng SF, Lin YP, Lin BY (2005) Characterization of AFLP sequences from regions of maize B chromosome defined by 12 B-10L translocations. Genetics 169:375–388

    Article  PubMed  CAS  Google Scholar 

  • Rubtsov NB, Karamysheva TV, Andreenkova OV et al (2004) Comparative analysis of micro and macro B chromosomes in the Korean field mouse Apodemus peninsulae (Rodentia, Murinae) performed by chromosome microdissection and FISH. Cytogenet Genome Res 106:289–294

    Article  PubMed  CAS  Google Scholar 

  • Sandery M, Forster J, Macadam S, Blunden R, Jones R, Brown S (1991) Isolation of a sequence common to A-and B-chromosomes of rye (Secale cereale) by microcloning. Plant Mol Biol Rep 9:21–30

    Article  CAS  Google Scholar 

  • Santos JL (1980) Variación de la heterocromatina constitutiva en el cariotipo de los Acridoidea y su efecto en el comportamiento cromosómico en meiosis. Ph.D. thesis, Universidad Complutense de Madrid, Spain

  • Sharbel TF, Green DM, Houben A (1998) B-chromosome origin in the endemic New Zealand frog Leiopelma hochstetteri through sex chromosome devolution. Genome 41:14–22

    Article  PubMed  CAS  Google Scholar 

  • Trifonov VA, Perelman PL, Kawada SI, Iwasa MA, Oda SI, Graphodatsky AS (2002) Complex structure of B-chromosomes in two mammalian species: Apodemus peninsulae (Rodentia) and Nyctereutes procyonoides (Carnivora). Chromosome Res 10:109–116

    Article  PubMed  CAS  Google Scholar 

  • Viseras E, Camacho JPM, Cano MI, Santos JL (1990) Relationship between mitotic instability and accumulation of B chromosomes in males and females of Locusta migratoria. Genome 33:23–29

    Google Scholar 

  • Wilkes TM, Francki MG, Langridge P, Karp A, Jones RN, Forster JW (1995) Analysis of rye B-chromosome structure using fluorescence in situ hybridization (FISH). Chromosome Res 3:466–472

    Article  PubMed  CAS  Google Scholar 

  • Yang F, O’Brien PCM, Milne BS et al (1999) A complete comparative chromosome map for the dog, red fox, and human and its integration with canine genetic maps. Genomics 62:189–202

    Article  PubMed  CAS  Google Scholar 

  • Ziegler CG, Lamatsch DK, Steinlein C, Engel W, Schartl M, Schmid M (2003) The giant B chromosome of the cyprinid fish Alburnus alburnus harbours a retrotransposon-derived repetitive DNA sequence. Chromosome Res 11:23–35

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

We thank David Martinez for language corrections, Tatiana López for technical assistance, and W. Traut and two anonymous reviewers for their perceptive criticism. This study was supported by grants from the Spanish Ministerio de Ciencia y Tecnología (CGL2006-06307 and CGL2006-05308/BOS, cofunded by the European Regional Development Fund) and Plan Andaluz de Investigación (P06-CVI-1664, BIO 165 and BIO 220).

Author information

Authors and Affiliations

  1. Departamento de Genética, Universidad de Granada, 18071, Granada, Spain

    María Teruel, Josefa Cabrero, Eugenia E. Montiel & Juan Pedro M. Camacho

  2. Departamento de Biología Experimental, Universidad de Jaén, Andalusia, Spain

    Manuel J. Acosta & Antonio Sánchez

Authors
  1. María Teruel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Josefa Cabrero
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Eugenia E. Montiel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Manuel J. Acosta
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Antonio Sánchez
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Juan Pedro M. Camacho
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Juan Pedro M. Camacho.

Additional information

Responsible Editor: Walther Traut.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Teruel, M., Cabrero, J., Montiel, E.E. et al. Microdissection and chromosome painting of X and B chromosomes in Locusta migratoria . Chromosome Res 17, 11–18 (2009). https://doi.org/10.1007/s10577-008-9001-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10577-008-9001-2

Keywords

Search

Navigation