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Effect of Induction Time on the Proliferation and Differentiation of Induced Schwann-Like Cells from Adipose-Derived Stem Cells

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A Correction to this article was published on 17 November 2022

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Abstract

To compare how different induction time takes effect on the proliferation and secretion ability of adipose-derived stem cell (ADSC)–induced Schwann-like cells (iSCs), ADSCs were isolated from healthy adult female rats. Flow cytometry (FCM) was performed to detect the ADSC-positive markers CD29, CD44, and CD90 and the negative marker CD45. iSC induction medium was used to culture the ADSCs. S-100, GFAP, MBP, and P75 were detected by immunofluorescence staining to identify iSC differentiation. Cell morphological changes were observed by an inverted microscope after induction. An MTS assay was used to evaluate the cell proliferation ability. Western blot analyses of caspase-3/cleaved caspase-3 and FCM were applied to assess cell apoptosis. Co-culture system of PC12 and ADSCs or iSCs was established to analyse the biological function of iSCs. Among the examined proteins, S-100, GFAP, MBP, and P75 were expressed in iSCs. After day 7, the cell proliferation rate was significantly lower than that before induction, and on day 19, the proliferation rate of iSCs was lower than 50% of the proliferation rate before induction (OD value = 0.016 ± 0.003 vs. 0.400 ± 0.004, p < 0.01). Starting from day 19, P21, P53, Apoj, S100, Gdnf, and Mbp all consistently showed a trend toward increased expression. Secretion of NGF, MBP, and BDNF was more enhanced at 19 days than that at 7 days. In co-culture system, the induction effect of iSCs was more pronounced at 19 days than that at 7 days, and the difference was statistically significant (55.40 ± 4.50 μm vs 37.15 ± 3.75 μm, p < 0.01). In conclusion, the proliferation ability of ADSC-derived iSCs was negatively correlated with the induction time, while the expression of SC marker proteins was positively correlated. Therefore, iSCs are suitable for use at 19 days after induction.

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Funding

This study was supported by the Natural Science Foundation of Guangdong Province (2018A0303130217); a Doctoral Start-up Project of the Natural Science Foundation of Guangdong Province (2017A030310302); the National Natural Science Foundation of China (81201546, 81901024); and the Undergraduate Teaching Quality Engineering Program of the First Affiliated Hospital of Sun Yat-sen University (18832601-0905).

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Author notes

  1. Chau Wei Wong and Yangbin Xu contributed equally as co-first authors.

Authors and Affiliations

  1. Department of Plastic Surgery, The First Affiliated Hospital of Sun Yat-Sen University, No. 58 Zhongshan Road 2, Guangzhou, 510080, China

    Chau Wei Wong, Yangbin Xu, Xiangxia Liu, Shuqia Xu, Yi Zhang & Zhaowei Zhu

  2. Department of Orthopedics, The Third Affiliated Hospital of Sun Yat-Sen University, No. 600 Tianhe Road, Tianhe District, Guangzhou, 510000, China

    Bo He

Authors
  1. Chau Wei Wong
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  4. Shuqia Xu
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  5. Yi Zhang
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Contributions

CWW cultured the cells and collected the specimens. XL and BH analysed and interpreted the experimental data. SX and YZ were responsible for the statistical analyses. BH, ZZ and YX designed the research, analysed the data and edited the manuscript. BH revised the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Yangbin Xu, Zhaowei Zhu or Bo He.

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Conflict of interest

All authors have no conflicts of interest to declare.

Ethical Approval

This study was approved by the Ethics Committee of the First Affiliated Hospital of Sun Yat-sen University, China (Approval number: [2013] A-055).

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The original online version of this article was revised: the reference Huang et al., 2019 is included in the article and it is cited in the caption of Fig. 5.

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Wong, C.W., Xu, Y., Liu, X. et al. Effect of Induction Time on the Proliferation and Differentiation of Induced Schwann-Like Cells from Adipose-Derived Stem Cells. Cell Mol Neurobiol 40, 1105–1116 (2020). https://doi.org/10.1007/s10571-020-00795-5

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