Abstract
Some anticonvulsant drugs are associated with cognitive ability in patients; Topiramate (TPM) is well known as an effective anticonvulsant agent applied in clinical settings. However, the effect of TPM on the cognitive function is rarely studied. In this study, we aimed to observe the effects of TPM on cell proliferation and neuronal differentiation in the dentate gyrus (DG) of the d-galactose-induced aging mice by Ki-67 and doublecortin (DCX) immunohistochemistry. The study is divided into four groups including control, d-galactose-treated group, 25 and 50Â mg/kg TPM-treated plus d-galactose-treated groups. We found, 50Â mg/kg (not 25Â mg/kg) TPM treatment significantly increased the numbers of Ki-67+ cells and DCX immunoreactivity, and improved neuroblast injury induced by d-galactose treatment. In addition, we also found that decreased immunoreactivities and protein levels of antioxidants including superoxide dismutase and catalase induced by d-galactose treatment were significantly recovered by 50Â mg/kg TPM treatment in the mice hippocampal DG (PÂ <Â 0.05). In conclusion, our present results indicate that TPM can ameliorate neuroblast damage and promote cell proliferation and neuroblast differentiation in the hippocampal DG via increasing SODs and catalase levels in the d-galactose mice.
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Acknowledgments
This study was supported by the National Natural Science Foundation of China (81401005), The National Natural Science Foundation of Jiangsu Province of China (BK20140494), and the University Natural Science Research General Project of Jiangsu Province (14KJB310027).
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Hui Shen and Jie Wang have contributed equally to this article.
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Shen, H., Wang, J., Jiang, D. et al. Topiramate Improves Neuroblast Differentiation of Hippocampal Dentate Gyrus in the d-Galactose-Induced Aging Mice via Its Antioxidant Effects. Cell Mol Neurobiol 37, 869–877 (2017). https://doi.org/10.1007/s10571-016-0424-6
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DOI: https://doi.org/10.1007/s10571-016-0424-6