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Angiotensin-(1–7) Via the Mas Receptor Alleviates the Diabetes-Induced Decrease in GFAP and GAP-43 Immunoreactivity with Concomitant Reduction in the COX-2 in Hippocampal Formation: An Immunohistochemical Study

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Abstract

We have previously shown that chronic treatment with angiotensin-(1–7) [Ang-(1–7)] can prevent diabetes-induced cardiovascular dysfunction. However, effect of Ang-(1–7) treatment on diabetes-induced alterations in the CNS is unknown. The aim of this study was to test the hypothesis that treatment with Ang-(1–7) can produce protection against diabetes-induced CNS changes. We examined the effect of Ang-(1–7) on the number of cyclooxygenase-2 (COX-2) immunoreactive neurons and the glial fibrillary acidic protein (GFAP)-immunoreactive astrocytes and assessed the changes in the neuronal growth-associated protein-43 (GAP-43) of the hippocampal formation in streptozotocin-induced diabetes in rats. Animals were sacrificed 30 days after induction of diabetes and/or treatment with Ang-(1–7). Ang-(1–7) treatment significantly prevented diabetes-induced decrease in the number of GFAP immunoreactive astrocytes and GAP-43 positive neurons in all hippocampal regions. Co-administration of A779, a selective Ang-(1–7) receptor antagonist, inhibited Ang-(1–7)-mediated protective effects indicating that Ang-(1–7) produces its effects through activation of receptor Mas. Further, Ang-(1–7) treatment through activation of Mas significantly prevented diabetes-induced increase in the number of the COX-2 immunolabeled neurons in all sub-regions of the hippocampus examined. These results show that Ang-(1–7) has a protective role against diabetes-induced changes in the CNS.

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Acknowledgments

This work was financially supported by Department of Anatomy, Faculty of Medicine, Kuwait University, Kuwait.

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Authors and Affiliations

  1. Department of Anatomy, Faculty of Medicine, Kuwait University, P. O. Box 24923, Safat, 13110, Kuwait

    Waleed M. Renno, Preethi George & Asmaa A. Abu-Ghefreh

  2. Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Kuwait University, Safat, Kuwait

    Anwar G. Al-Banaw

  3. Department of Pharmacology & Toxicology, Faculty of Medicine, Kuwait University, Safat, Kuwait

    Saghir Akhtar & Ibrahim F. Benter

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  1. Waleed M. Renno
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  2. Anwar G. Al-Banaw
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Correspondence to Waleed M. Renno.

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Renno, W.M., Al-Banaw, A.G., George, P. et al. Angiotensin-(1–7) Via the Mas Receptor Alleviates the Diabetes-Induced Decrease in GFAP and GAP-43 Immunoreactivity with Concomitant Reduction in the COX-2 in Hippocampal Formation: An Immunohistochemical Study. Cell Mol Neurobiol 32, 1323–1336 (2012). https://doi.org/10.1007/s10571-012-9858-7

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