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EGCG Ameliorates the Suppression of Long-Term Potentiation Induced by Ischemia at the Schaffer Collateral-CA1 Synapse in the Rat

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Abstract

The function of Epigallocatechin gallate (EGCG), a main component of green tea, has been widely investigated, amelioration of synaptic transmission and neuroprotective effects against ischemia-induced brain damage among others. However, the mechanism underlying is still unveiled. We investigated the effects of EGCG on high frequency stimulation-induced long-term potentiation (LTP) in the Schaffer collateral-CA1 synapse with or without cerebral ischemia injury induced by middle cerebral artery occlusion (MCAO) in vivo to examine the possible relations between EGCG and synaptic transmission. Application of EGCG modulated synaptic transmission and produced a dose-dependent improvement of the induction of LTP. However, relative high-dose EGCG can block the induction of LTP at the Schaffer collateral-CA1 synapse in normal rat in vivo. In addition, the effects of EGCG were observed on the infarct volume and neurological deficit in rats subjected to MCAO; furthermore, the cell viability of primary cultured rat hippocampal and cortical neurons suffered from oxygen–glucose deprivation were evaluated with MTT and LDH assay, which showed significant neuroprotective properties in vitro. Surprisingly, the contents of the glutamate (Glu), glycine (Gly), and gamma-aminobutyric acid amino acids were totally disequilibrated before and after cerebral ischemia injury and could be rebalanced to original level by application of EGCG. Our results suggest that EGCG is able to improve the efficiency of synaptic transmission in cerebral ischemia injury with attenuated effect related to the neuroprotection of EGCG through regulating excitatory and inhibitory amino acid balance.

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Acknowledgments

This study was supported by the National Foundation of Nature and Science of China (No. 81173038) and by the Foundation of Central Authorities of an Institution of Higher Learning of Scientific Research Special Fund (No. 2011TS073).

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Authors and Affiliations

  1. Department of Pharmacology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan, 430030, People’s Republic of China

    Jie Ding, Gang Fu, Yan Zhao, Zhenyong Cheng, Yang Chen, Bo Zhao, Wei He & Lian-Jun Guo

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  1. Jie Ding
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  2. Gang Fu
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  3. Yan Zhao
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  4. Zhenyong Cheng
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  5. Yang Chen
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  6. Bo Zhao
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  7. Wei He
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  8. Lian-Jun Guo
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Correspondence to Lian-Jun Guo.

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Ding, J., Fu, G., Zhao, Y. et al. EGCG Ameliorates the Suppression of Long-Term Potentiation Induced by Ischemia at the Schaffer Collateral-CA1 Synapse in the Rat. Cell Mol Neurobiol 32, 267–277 (2012). https://doi.org/10.1007/s10571-011-9758-2

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