Abstract
It would be very useful to be able to classify brain tumor stem cells (BTSCs) by certain criteria to afford the design of specific or individualized treatment. Here, we studied two BTSC lines with differing biological and molecular features and whose respective features were well preserved after cryopreservation as single cells in SFM or 90% serum with 10% DMSO, a method not previously reported. The resuscitated BTSCs shared properties indistinguishable from their respective parental cells, including tumor sphere forming potentials, growth and differentiation properties, and tumorigenesis in vivo. The two cell lines also had differing molecule profiles, which can be well preserved after cryopreservation, similar to that of their respective primary tumors. Therefore, BTSCs from different patients, that have their own properties, were well retained by the present cryopreservation method, which might be a useful and reliable method for preserving BTSCs for long-term studies, such as classification and specific therapy design.
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Abbreviations
- bFGF:
-
Basic fibroblast growth factor
- BTSCs:
-
Brain tumor stem cells
- CSCs:
-
Cancer stem cells
- DMSO:
-
Dimethyl sulfoxide
- EGF:
-
Epidermal growth factor
- NSCs:
-
Neural stem cells
- SFM:
-
Serum-free medium containing EGF, bFGF and B27 supplement
- SFM-CryoP-TS:
-
Tumor spheres formed by cells cryopreserved in SFM and 10% DMSO
- S-CryoP-TS:
-
Tumor spheres formed by cells cryopreserved in 90% serum and 10% DMSO
- TSs:
-
Tumor spheres
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Acknowledgments
We thank Ms. Junli Huo, Ms. Juan Li, Mrs. Yufen Shi, and Mrs. Xiaoyan Chen for their technical assistance in cell culture, immunohistochemistry, and QPCR. We appreciate Dr. Fucheng Ma (Department of Pathology, Xijing Hospital, Fourth Military Medical University) for confirming the pathological diagnosis of tumor samples and xenografts. We also thank Dr. Yao Yang (Institute of Neurosciences, The Fourth Military Medical University) for helpful suggestions. This study is supported by funds from the Fourth Military Medical University.
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Xing-gang Mao, Geng Guo and Peng Wang contributed equally to this article.
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Mao, Xg., Guo, G., Wang, P. et al. Maintenance of Critical Properties of Brain Tumor Stem-like Cells After Cryopreservation. Cell Mol Neurobiol 30, 775–786 (2010). https://doi.org/10.1007/s10571-010-9505-0
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DOI: https://doi.org/10.1007/s10571-010-9505-0