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Glutamate Uptake Is Stimulated by Extracellular S100B in Hippocampal Astrocytes

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Summary

  1. 1.

    S100B is a calcium-binding protein expressed and secreted by astrocytes, which has been implicated in glial-neuronal communication. Extracellular S100B appears to protect hippocampal neurons against toxic concentrations of glutamate. Here we investigated a possible autocrine role of S100B in glutamate uptake activity.

  2. 2.

    Astrocyte cultures were prepared of hippocampi from neonate Wistar rats. [3H] Glutamate uptake was measured after addition of S100B protein, antibody anti-S100B or TRTK-12, a peptide that blocks S100B activity mediated by the C-terminal region.

  3. 3.

    Antibody anti-S100B addition decreased glutamate uptake measured 30 min after medium replacement, without affecting cell integrity or viability. Moreover, low levels of S100B (less than 0.1 ng/mL) stimulated glutamate uptake measured immediately after medium replacement.

  4. 4.

    This finding reinforces the importance of astrocytes in the glutamatergic transmission, particularly the role of S100B neuroprotection against excitotoxic damage.

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Correspondence to Carlos-Alberto Gonçalves.

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Tramontina, F., Tramontina, A.C., Souza, D.F. et al. Glutamate Uptake Is Stimulated by Extracellular S100B in Hippocampal Astrocytes. Cell Mol Neurobiol 26, 81–86 (2006). https://doi.org/10.1007/s10571-006-9099-8

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  • DOI: https://doi.org/10.1007/s10571-006-9099-8

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