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Optimization of cellulase production by a novel endophytic fungus Pestalotiopsis microspora TKBRR isolated from Thalakona forest

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Abstract

The present work focused on cellulase production in solid-state fermentation by a fungal isolate of Thalakona forest in Andhra Pradesh identified as Pestalotiopsis microspora TKBRR. Under normal conditions, yields of Filter Paperase (FPase), Carboxymethyl Cellulase (CMCase) and β-glucosidase (BGL) produced by P. microspora TKBRR in SSF were 13, 12 and 11 U/g DS whereas the corresponding yields of FPase, CMCase and BGL after optimization of process conditions and medium components through one stage approach—Central Composite Design in RSM were 15, 16 and 13 U/g DS, respectively in the present study. There was a marginal improvement in yields of cellulolytic enzymes by 2–4 units with the least interactions between factors. Of the four factors tested in the present study, only temperature and pH influenced production of the cellulolytic enzymes and P. microspora TKBRR in SSF exhibited a higher ratio of BGL to FPase in comparison to other cellulolytic organisms reported.

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Abbreviations

CCD:

Central composite design

RSM:

Response surface methodology

FPase:

Filter paper cellulase

SSF:

Solid-state fermentation

CMC:

Carboxymethylcellulose

CMcase:

Carboxymethylcellulase

BGL:

β-Glucosidase

U/gDS:

Unit per gram dry weight substrate

ANOVA:

Analysis of variance

OFAT:

One factor at a time

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Authors and Affiliations

  1. Department of Microbiology, Sri Krishnadevaraya University, Anantapuramu, 515 003, India

    Praveen Kumar Reddy Goukanapalle, Dileep Kumar Kanderi, B. S. Shanthi Kumari & Rajasekhar Reddy Bontha

  2. Department of Biotechnology, Faculty of Agro-industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Girinath Rajoji

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  1. Praveen Kumar Reddy Goukanapalle
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  2. Dileep Kumar Kanderi
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  3. Girinath Rajoji
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  4. B. S. Shanthi Kumari
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  5. Rajasekhar Reddy Bontha
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Correspondence to Rajasekhar Reddy Bontha.

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Fig. S1 Screening of fungal cultures for cellulolytic activities (DOCX 113 kb)

10570_2020_3220_MOESM2_ESM.docx

Fig. S2 Three dimensional response surface plots showing (S2a) effect of temperature and pH (S2b) effect of temperature and moisture content (S2c) effect of temperature and urea concentration (S2d) effect of pH and moisture content (S2e) effect of pH and urea concentration (S2f) effect of moisture content and urea concentration on the production of FPase by P. microspora TKBRR under solid state fermentation (DOCX 731 kb)

10570_2020_3220_MOESM3_ESM.docx

Fig. S3 Three dimensional response surface plots showing (S3a) effect of temperature and pH (S3b) effect of temperature and moisture content (S3c) effect of temperature and urea concentration (S3d) effect of pH and moisture content (S3e) effect of pH and urea concentration (S3f) effect of moisture content and urea concentration on the production of CMCase by P. microspora TKBRR under solid state fermentation (DOCX 848 kb)

Table S1. Factors and their levels for CCD (DOCX 11 kb)

10570_2020_3220_MOESM5_ESM.docx

Table S2. Influence of incubation period on production of cellulolytic enzymes by P. microspora TKBRR in SSF (DOCX 12 kb)

Table S3. Influence of temperature on production of cellulolytic enzymes by P. microspora TKBRR in SSF (DOCX 12 kb)

Table S4. Influence of pH on production of cellulolytic enzymes by P. microspora TKBRR in SSF (DOCX 12 kb)

10570_2020_3220_MOESM8_ESM.docx

Table S5. Influence of Nitrogen sources on production of cellulolytic enzymes by P. microspora TKBRR in SSF (DOCX 12 kb)

10570_2020_3220_MOESM9_ESM.docx

Table S6. Influence of various surfactants on production of cellulolytic enzymes by P. microspora TKBRR in SSF (DOCX 12 kb)

Table S7. Analysis of variance for FPase (Partial sum of squares) (DOCX 14 kb)

Table S8. Analysis of variance for CMCase (Partial sum of squares) (DOCX 13 kb)

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Goukanapalle, P.K.R., Kanderi, D.K., Rajoji, G. et al. Optimization of cellulase production by a novel endophytic fungus Pestalotiopsis microspora TKBRR isolated from Thalakona forest. Cellulose 27, 6299–6316 (2020). https://doi.org/10.1007/s10570-020-03220-8

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