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Role of Yersinia enterocolitica heat-stable enterotoxin (Y-STa) on differential regulation of nuclear and cytosolic calcium signaling in rat intestinal epithelial cells

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Abstract

The heat-stable enterotoxin (Y-STa) produced by the pathogenic strains of Yersinia enterocolitica is a causative agent of secretory diarrhea. We have reported earlier that Y-STa-induced inositol trisphosphate-mediated cytosolic calcium rise occurs in rat intestinal epithelial cells. In the present communication, the involvement of a nuclear calcium store in the action mechanism of Y-STa in rat intestinal epithelial cells has been shown. Calcium imaging with time series confocal microscopy shows that Y-STa stimulates both the nuclear and cytosolic calcium levels in rat intestinal epithelial cells where a rise in nuclear calcium precedes the cytosolic events. Moreover, Y-STa stimulates both cytosolic and nuclear inositol trisphosphate (IP3) levels in a time-dependent manner. Western blot and immunocytochemical analysis reveal a higher density of IP3 receptor type II in the nuclear membrane compared to the cytosol, which may be the cause of an early rise of the nuclear calcium level. Therefore, it is suggested that Y-STa regulates the nuclear and cytosolic calcium signals in a distinct temporal manner in rat intestinal epithelial cells.

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Abbreviations

2-APB:

2-aminoethyl diphenylborinate

BAPTA-AM:

1,2-bis(2-aminophenoxy) ethane-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl ester)

Fluo-3/AM:

fluo-3 acetoxymethyl ester

IP3 :

inositol trisphosphate

U-73122:

1-[6-[{(17β)-3-methoxyestra-1, 3,5[10]-trien-17-yl} amino] hexyl]-1H-pyrrole-2, 5-dione

U-73343:

1-[6-[{(17β)-3-methoxyestra-1, 3,5[10]-trien-17-yl} amino] hexyl]-2,5-pyrrolidinedione

Y-STa:

Yersinia enterocolitica heat-stable enterotoxin

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Acknowledgments

We wish to thank Dr. G.B.Nair, Director, National Institute of Cholera and Enteric Diseases, Calcutta, for encouragement throughout the studies. The first author (SS) is a recipient of the Senior Research Fellowship of Indian Council of Medical Research. The second author (PC) is a recipient of the Junior Research Fellowship of University Grants Commission, Government of India.

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  1. Division of Pathophysiology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T.Road, Scheme-XM, Beliaghata, Calcutta, 700010, India

    Subhrajit Saha, Pinki Chowdhury, Arindam Mazumdar, Amit Pal, Pradeep Das & Manoj K. Chakrabarti

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  1. Subhrajit Saha
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  2. Pinki Chowdhury
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  3. Arindam Mazumdar
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  6. Manoj K. Chakrabarti
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Correspondence to Manoj K. Chakrabarti.

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Saha, S., Chowdhury, P., Mazumdar, A. et al. Role of Yersinia enterocolitica heat-stable enterotoxin (Y-STa) on differential regulation of nuclear and cytosolic calcium signaling in rat intestinal epithelial cells. Cell Biol Toxicol 25, 297–308 (2009). https://doi.org/10.1007/s10565-008-9084-8

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