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Evaluation of the biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa on human umbilical cord CD146+ stem cells and stem cell-based decellularized matrix

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Abstract

This study aims to evaluate the CD146+ stem cells obtained from the human umbilical cord and their extracellular matrix proteins on in vitro Pseudomonas aeruginosa and Staphylococcus aureus biofilms to understand their possible antimicrobial activity. CD146+ stem cells were determined according to cell surface markers and differentiation capacity. Characterization of the decellularized matrix was done with DAPI, Masson’s Trichrome staining and proteome analysis. Cell viability/proliferation of cells in co-cultures was evaluated by WST-1 and crystal-violet staining. The effects of cells and decellularized matrix proteins on biofilms were investigated on a drip flow biofilm reactor and their effects on gene expression were determined by RT-qPCR. We observed that CD146/105+ stem cells could differentiate adipogenically and decellularized matrix showed negative DAPI and positive collagen staining with Masson’ s Trichrome. Proteome analysis of the decellularized matrix revealed some matrix components and growth factors. Although the decellularized matrix significantly reduced the cell counts of P. aeruginosa, no significant difference was observed for S. aureus cells in both groups. Supporting data was obtained from the gene expression results of P. aeruginosa with the significant down-regulation of rhlR and lasR. For S. aureus, icaADBC genes were significantly up-regulated when grown on the decellularized matrix.

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Acknowledgements

We would like to thank Koc University Research Center for Translational Medicine.

Funding

This work was supported by Hacettepe University, Scientific Research Project Coordination Unit, Grant Number: TYL-2019-17802.

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Authors and Affiliations

  1. Department of Stem Cell Sciences, Hacettepe University Graduate School of Health Sciences, 06100, Sihhiye, Ankara, Turkey

    Nur Kübra Çankirili & Betül Çelebi-Saltik

  2. Center for Stem Cell Research and Development, Hacettepe University, 06100, Sihhiye, Ankara, Turkey

    Nur Kübra Çankirili & Betül Çelebi-Saltik

  3. Department of Pharmaceutical Microbiology, Hacettepe University Faculty of Pharmacy, 06100, Sihhiye, Ankara, Turkey

    Didem Kart

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  1. Nur Kübra Çankirili
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Correspondence to Betül Çelebi-Saltik.

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In this study, umbilical cord samples were obtained immediately after delivery from non-complicated pregnant women in Hacettepe University Faculty of Medicine, Department of Obstetrics and Gynecology. Hacettepe University Local Non-Interventional Clinical Researches Ethics Committee approved human material use (GO18/446-22) for this in vitro study.

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Fig. Suppl. 1

Antibacterial activity of decellularized extracellular matrix. Decellularized extracellular matrix in cell culture flasks (A), Disk-shaped pieces of decellularized extracellular matrix cut by using a sterile punch (B) No inhibition zones were observed both for S. aureus (left side) and P. aeruginosa (right side) (C, n = 4) (TIFF 1145 kb)

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Çankirili, N.K., Kart, D. & Çelebi-Saltik, B. Evaluation of the biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa on human umbilical cord CD146+ stem cells and stem cell-based decellularized matrix. Cell Tissue Bank 21, 215–231 (2020). https://doi.org/10.1007/s10561-020-09815-6

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