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Utility of ankyrin 3 as a prognostic marker in androgen-receptor-positive breast cancer

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Abstract

Purpose

Androgen receptor (AR) and AR signaling pathways are thought to play a role in breast cancer (BC) and are potentially related to treatment responses and outcomes. Ankyrin 3 (ANK3) is associated with AR stability in cancer cells. In the present study, we investigated the clinicopathological utility of ANK3 expression with emphasis on AR and its associated signalling pathway at transcriptomic and proteomic phases.

Patients and methods

The Molecular Taxonomy of Breast Cancer International Consortium (METABRIC) cohort (n = 1980) and The Cancer Genome Atlas (TCGA) dataset (n = 1039) were used to assess the expression and significance of ANK3 mRNA and other AR signalling pathway-associated gene signature. Using immunohistochemistry, ANK3 protein expression was evaluated in large (n = 982) cohort of early-stage BC with long-term follow-up and compared with clinicopathological characteristics and its prognostic value in the whole cohort and the subgroups stratified by AR protein expression.

Results

An AR-related gene signature was developed, comprising 20 genes, which included ANK3. This AR-related gene signature was significantly associated with AR mRNA expression, oestrogen receptor, human epidermal growth factor receptor 2 (HER2) status and the patients’ outcomes. In tumours with high AR protein expression (n = 614), high ANK3 protein expression was significantly associated with progesterone receptor positivity and it was independently associated with the good outcomes (p = 0.025).

Conclusions

This study indicates that ANK3 is related to AR signalling pathway and is associated with BC prognosis.

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Acknowledgements

We thank the Nottingham Health Science Biobank and Breast Cancer Now Tissue Bank for the provision of tissue samples.

Funding

This study was funded by the University of Nottingham (Nottingham Life Cycle 6).

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Authors and Affiliations

  1. Division of Cancer and Stem Cells, Nottingham Breast Cancer Research Centre, School of Medicine, University of Nottingham, Nottingham, UK

    Sasagu Kurozumi, Chitra Joseph, Sara Raafat, Sultan Sonbul, Yousif Kariri, Sami Alsaeed, Marian Pigera, Mansour Alsaleem, Christopher C. Nolan, Simon J. Johnston, Mohammed A. Aleskandarany, Angela Ogden, Stewart G. Martin, Ian O. Ellis, Andrew R. Green & Emad A. Rakha

  2. Department of General Surgical Science, Gunma University Graduate School of Medicine, Gunma, Japan

    Sasagu Kurozumi, Takaaki Fujii & Ken Shirabe

  3. Department of Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt

    Sara Raafat

  4. Faculty of Medicine, Menoufyia University, Shebin El Kom, Egypt

    Mohammed A. Aleskandarany & Emad A. Rakha

  5. Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia

    Ibraheem Alshankyty

  6. Cancer Biology and Translational Research, Faculty of Medicine and Health Sciences, University of Nottingham, Nottingham, UK

    Nigel P. Mongan

  7. Department of Pharmacology, Weill Cornell Medicine, New York, USA

    Nigel P. Mongan

  8. Division of Cancer and Stem Cells, Department of Histopathology, School of Medicine, The University of Nottingham and Nottingham University Hospitals NHS Trust, Nottingham City Hospital, Nottingham, NG5 1PB, UK

    Emad A. Rakha

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  1. Sasagu Kurozumi
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  2. Chitra Joseph
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  3. Sara Raafat
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  4. Sultan Sonbul
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  7. Marian Pigera
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  8. Mansour Alsaleem
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  9. Christopher C. Nolan
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  11. Mohammed A. Aleskandarany
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  12. Angela Ogden
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  13. Takaaki Fujii
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  14. Ken Shirabe
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  15. Stewart G. Martin
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  16. Ibraheem Alshankyty
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  17. Nigel P. Mongan
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  18. Ian O. Ellis
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  19. Andrew R. Green
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  20. Emad A. Rakha
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Corresponding author

Correspondence to Emad A. Rakha.

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Conflict of interest

Ibraheem Alshankyty is a consultant/advisory board in Molecular Diagnostics Lab, College of Applied Med. Sci., KAU. All authors of this work declare that they have no conflict of interest.

Ethical approval

This study was approved by the Nottingham Research Ethics Committee 2 (Reference title: Development of a molecular genetic classification of breast cancer). All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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Kurozumi, S., Joseph, C., Raafat, S. et al. Utility of ankyrin 3 as a prognostic marker in androgen-receptor-positive breast cancer. Breast Cancer Res Treat 176, 63–73 (2019). https://doi.org/10.1007/s10549-019-05216-w

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