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LFA-1 gene polymorphisms are associated with the sporadic infiltrative duct breast carcinoma in Chinese Han women of Heilongjiang Province

  • Epidemiology
  • Published:
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Abstract

The infiltrative duct carcinoma (IDC) is the most common malignant breast cancer in females and genetic factors appear to play a significant role in the susceptibility of IDC. The LFA-1 is a crucial co-stimulatory molecule in immune system and may affect the development of breast IDC. In order to clarify the association of LFA-1 polymorphisms with IDC, a case–control study was conducted in women from Heilongjiang Province, Northeast of China. We scrutinized four genetic polymorphisms in LFA-1 gene, which may influence the activity and function of LFA-1. Our research subjects consist of 537 cases with IDC and 577 age-matched healthy controls. Genotypes were determined by PCR-RFLP. Data were analyzed using the χ2 test by SPSS 13.0 and Haploview 4.1 softwares. The association between LFA-1 polymorphisms and the clinical features of IDC was analyzed. In rs2230433, the frequency of GG genotype and G allele was lower in cases than in controls (P = 0.0316 and 0.0480). And rs2230433, CG genotype was higher in cases (P = 0.0397). In rs8058823, the frequency of AA genotype and A allele was lower in cases than in controls (P = 0.00000418 and 0.00000267). And rs8058823, AG genotype was higher in cases (P = 0.00000747). The frequency of haplotype CCGA was lower in patients. Significant association was shown between the four SNPs of LFA-1 gene and estrogen receptor (ER), progesterone receptor (PR), C-erbB-2, and P53 statuses. In addition, no association was found between LFA-1 gene polymorphisms and tumor size, and neither was it between LFA-1 gene polymorphisms and lymph node metastasis. Our results primarily suggested that LFA-1 gene polymorphisms may predict the sporadic breast IDC risk and prognosis factors in Chinese Han women in Heilongjiang Province.

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Abbreviations

LFA-1:

Lymphocyte function-associated antigen-1

IDC:

Infiltrative duct carcinoma

OR:

Odds ratio

CI:

Confidence interval

HWE:

Hardy–Weinberg equilibrium

LN:

Lymph node

PCR-RFLP:

Polymerase chain reaction-restriction fragment length polymorphism

ER:

Estrogen receptor

PR:

Progesterone receptor

SNP:

Single nucleotide polymorphism

TZ:

Tumor size

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Acknowledgments

Zhenkun Fu performed the primer design and wrote the drafts. Mingli Jiao contributed statistical analysis. Mingyan Zhang collected the patient and control blood samples. Fengyan Xu and Weiguang Yuan performed the PCR-RFLP experiments. Da Pang and Dianjun Li conceived the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript. This study was supported by a grant from the National Natural Science Foundation of China (31070780), the Heilongjiang Province Foundation of Education Department (11522001) and the Major Project of Technology Department, Heilongjiang Province (GB05C402). We thank all patients and healthy volunteers for providing blood samples. We are grateful for the collaboration received from the participating hospitals and their staff.

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Authors and Affiliations

  1. Department of Immunology, Harbin Medical University, Harbin, 150081, China

    Zhenkun Fu, Mingyan Zhang, Fengyan Xu & Dianjun Li

  2. Institute of Cancer Prevention and Treatment, Harbin Medical University, Harbin, 150081, China

    Zhenkun Fu, Weiguang Yuan, Da Pang & Dianjun Li

  3. Department of Social Medicine, Health Management College of Harbin Medical University, Harbin, 150081, China

    Mingli Jiao

  4. Department of Surgery, The Third Affiliated Hospital of Harbin Medical University, Harbin, 150081, China

    Da Pang

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  1. Zhenkun Fu
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Correspondence to Da Pang or Dianjun Li.

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The first two authors have contributed equally to this work.

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Fu, Z., Jiao, M., Zhang, M. et al. LFA-1 gene polymorphisms are associated with the sporadic infiltrative duct breast carcinoma in Chinese Han women of Heilongjiang Province. Breast Cancer Res Treat 127, 265–271 (2011). https://doi.org/10.1007/s10549-010-1203-6

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  • DOI: https://doi.org/10.1007/s10549-010-1203-6

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