Abstract
The quantitative and qualitative analysis of circulating tumor cells (CTCs) has the potential to improve the clinical management of several cancers, including prostate cancer. As such, there is much interest in the isolation of CTCs from the peripheral blood of cancer patients. We report the design, fabrication, and proof-of-principle testing of an integrated permalloy-based microfluidic chip for immunomagnetic isolation of blood-borne prostate cancer cells using an antibody targeting prostate surface membrane antigen (PSMA). The preliminary results using spiked blood samples indicate that the proposed device is consistently capable of isolating prostate cancer cells with high sensitivity (up to 98 %) at clinically relevant low concentrations (down to 20 cells/mL) and an acceptable throughput (100 μL/min).
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Acknowledgment
This work made use of the 4D LABS shared facilities supported by the Canada Foundation for Innovation (CFI), British Columbia Knowledge Development Fund (BCKDF), Western Economic Diversification Canada (WD), and Simon Fraser University (SFU). We would also like to acknowledge CMC Microsystems for the provision of products and services, including CAD and simulation tools, which facilitated this research. This work was supported in part by a Discovery Grant, RGPIN/298219-2012 (Park), from the Natural Science and Engineering Research Council of Canada (NSERC).
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All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted (Protocol#: 1056E-12, University Animal Care Committee, Simon Fraser University).
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Esmaeilsabzali, H., Beischlag, T.V., Cox, M.E. et al. An integrated microfluidic chip for immunomagnetic detection and isolation of rare prostate cancer cells from blood. Biomed Microdevices 18, 22 (2016). https://doi.org/10.1007/s10544-016-0041-7
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DOI: https://doi.org/10.1007/s10544-016-0041-7