Abstract
A microfluidic preconcentration device comprising a microchannel and a surface-patterned nanoscale Nafion membrane is proposed. Given the application of an electric field across the chip, the nanopore within Nafion membrane becomes ion selective due to an overlapping of the electric double layer. The resulting difference in flux of the co- and counter-ions within the membrane nanopore prompts the formation of a concentration gradient and leads to a gradual accumulation of the co-ions at the micro-nano junction. It is shown experimentally that the rate of concentration and the preconcentration factor both increase with an increasing electrical field intensity. The preconcentration performance in a straight microchannel is compared with that in a convergent microchannel using fluorescein disodium salt dehydrate and Fluorescein isothiocyanate (FITC)-labeled bovine serum albumin samples. The results show that the reduced cross-sectional area of the convergent microchannel increases the preconcentration factor compared to that obtained in a straight microchannel and yields a significant reduction in the preconcentration time.
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References
N.L. Anderson, N.G. Anderson, Mol Cell Proteomics 1, 845 (2002)
J. Astorga-Wells, H. Swerdlow, Anal Chem 75, 5207 (2003)
N. Beyor, T.S. Seo, P. Liu, R.A. Mathies, Biomed Microdevices 10, 909 (2008)
D. Bottenus, T.Z. Jubery, P. Dutta, C.F. Ivory, Electrophoresis 32, 550 (2011)
D.S. Burgi, R.L. Chien, Anal Chem 63, 2042 (1991)
C.L. Chen, R.J. Yang, Electrophoresis 33, 751 (2012)
H. Chun, T.D. Chung, J.M. Ramsey, Anal Chem 82, 6287 (2010)
R. Dhopeshwarkar, R.M. Crooks, D. Hlushkou, U. Tallarek, Anal Chem 80, 1039 (2008)
J.R. Du, Y.J. Juang, J.T. Wu, H.H. Wei, Biomicrofluidics 2, 044103 (2008)
P. Gebauer, P. Bocek, Electrophoresis 23, 3858 (2002)
G. Goet, T. Baier, S. Hardt, A.K. Sen, Biomicrofluidics 7, 044103 (2013)
A.V. Hatch, A.E. Herr, D.J. Throckmorton, J.S. Brennan, A.K. Singh, Anal Chem 78, 4976 (2006)
D. Hlushkou, R. Dhopeshwarkar, R.M. Crooks, U. Tallarek, Lab Chip 8, 1153 (2008)
K.W. Hoeman, J.J. Lange, G.T. Roman, D.A. Higgins, C.T. Culbertson, Electrophoresis 30, 3160 (2009)
K.D. Huang, R.J. Yang, Microfluid Nanofluid 5, 631 (2008)
C. Huang, H. Liu, N.H. Bander, B.J. Kirby, Biomed Microdevices 15, 941 (2013)
C.P. Jen, H.H. Chang, Biomicrofluidics 5, 034101 (2011)
J. Khandurina, S.C. Jacobson, L.C. Waters, R.S. Foote, J.M. Ramsey, Anal Chem 71, 1815 (1999)
S.J. Kim, J. Han, Anal Chem 80, 3507 (2008)
S.M. Kim, M.A. Burns, E.F. Hasselbrink, Anal Chem 78, 4779 (2006)
S.J. Kim, Y.-C. Wang, J. Lee, H. Jang, J. Han, Phys Rev Lett 99, 044501 (2007)
S.J. Kim, L.D. Li, J. Han, Langmuir 25, 7759 (2009)
P. Kim, S.J. Kim, J. Han, K.Y. Suh, Nano Lett 10, 16 (2010a)
S.J. Kim, S.H. Ko, K.H. Kang, J. Han, Nat Nanotechnol 5, 297 (2010b)
S.J. Kim, Y.-A. Song, J. Han, Chem Soc Rev 39, 912 (2010c)
K.B. Kim, J.H. Han, H. Choi, H.C. Kim, T.D. Chung, Small 8, 378 (2012)
J.H. Lee, Y.-A.K. Song, J. Han, Lab Chip 8, 596 (2008)
K.F. Lei, K.-H. Chen, Y.-C. Chang, Biomed Microdevices 16, 325 (2014)
V. Liu, Y.-A. Song, J. Han, Lab Chip 10, 1485 (2010)
D. Mampallil, D. Tiwari, D. van den Ende, F. Mugele, Biomicrofluidics 7, 044102 (2013)
J.C. McDonald, D.C. Duffy, J.R. Anderson, D.T. Chiu, H. Wu, O.J.A. Schueller, G.M. Whitesides, Electrophoresis 21, 27 (2000)
R.J. Meagher, N. Thaitrong, Electrophoresis 33, 1236 (2012)
S. Mikkonen, M.K. Rokhas, J. Jacksen, A. Emmer, Electrophoresis 33, 3343 (2012)
P.N. Nge, W. Yang, J.V. Pagaduan, A.T. Woolley, Electrophoresis 32, 1133 (2011)
R.D. Oleschuk, L.L. Shultz-Lockyear, Y. Ning, D.J. Harrison, Anal Chem 72, 585 (2000)
Q. Pu, J. Yun, H. Temkin, S. Liu, Nano Lett 4, 1099 (2004)
D. Puchberger-Enengl, S. Podszun, H. Heinz, C. Hermann, P. Vulto, G.A. Urban, Biomicrofluidics 5, 044111 (2011)
J.P. Quirino, S. Terabe, Science 282, 465 (1998)
I. Rubinstein, L. Shtilman, J Chem Soc Farad T 2 75, 231 (1979)
R. Schoch, J. Han, P. Renaud, Rev Mod Phys 80, 839 (2008)
J.S. Shim, J. Geng, C.H. Ahn, P. Guo, Biomed Microdevices 14, 921 (2012)
S. Song, A.K. Singh, B.J. Kirby, Anal Chem 76, 4589 (2004)
Z. Svobodova, M.R. Mohamadi, B. Jankovicova, H. Esselmann, R. Verpillot, M. Otto, M. Taverna, J. Wiltfang, J.L. Viovy, Z. Bilkova, Biomicrofluidics 6, 024126 (2012)
V.M. Barraga’n, C.R. Bauza, J Colloid Interface Sci 205, 365 (1998)
Y.C. Wang, J. Han, Lab Chip 8, 392 (2008)
Y.-C. Wang, A.L. Stevens, J Han Anal Chem 77, 4293 (2005)
Y. Wang, K. Pant, Z. Chen, G. Wang, W.F. Diffey, P. Ashley, S. Sundaram, Microfluid Nanofluid 7, 683 (2009)
H. Xia, B. Mathew, T. John, H. Hegab, J. Feng, Biomed Microdevices 15, 519 (2013)
C. Yu, M.H. Davey, F. Svec, J.M.J. Frechet, Anal Chem 73, 5088 (2001)
H. Yu, Y. Lu, Y.G. Zhou, F.B. Wang, F.Y. He, X.H. Xia, Lab Chip 8, 1496 (2008)
H. Zhu, G. Wang, D. Xie, B. Cai, Y. Liu, X. Zhao, Biomed Microdevices 16, 479 (2014)
Acknowledgments
The authors gratefully acknowledge the financial support provided to this study by the Ministry of Science and Technology of Taiwan. Microfabrication facilities provided by the National Nano-Device Laboratory is also acknowledged.
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Chao, CC., Chiu, PH. & Yang, RJ. Preconcentration of diluted biochemical samples using microchannel with integrated nanoscale Nafion membrane. Biomed Microdevices 17, 25 (2015). https://doi.org/10.1007/s10544-015-9940-2
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DOI: https://doi.org/10.1007/s10544-015-9940-2