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Optimization of flow assisted entrapment of pollen grains in a microfluidic platform for tip growth analysis

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Abstract

A biocompatible polydimethylsiloxane (PDMS) biomicrofluidic platform is designed, fabricated and tested to study protuberance growth of single plant cells in a micro-vitro environment. The design consists of an inlet to introduce the cell suspension into the chip, three outlets to conduct the medium or cells out of the chip, a main distribution chamber and eight microchannels connected to the main chamber to guide the growth of tip growing plant cells. The test cells used here were pollen grains which produce cylindrical protrusions called pollen tubes. The goal was to adjust the design of the microfluidic network with the aim to enhance the uniformly distributed positioning of pollen grains at the entrances of the microchannels and to provide identical fluid flow conditions for growing pollen tubes along each microchannel. Computational fluid analysis and experimental testing were carried out to estimate the trapping efficiencies of the different designs.

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Acknowledgments

The authors acknowledge research support from the Fonds de recherche du Québec - Nature et Technologies (FQRNT) and Concordia Research Chair.

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Authors and Affiliations

  1. Optical-Bio Microsystem Laboratory, Department of Mechanical and Industrial Engineering, Concordia University, Montreal, QC, Canada, H3G 1M8

    Amir Sanati Nezhad, Mahmood Ghanbari, Carlos G. Agudelo, Muthukumaran Packirisamy & Rama B. Bhat

  2. Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, University of de Montreal, Montreal, Canada, H1X 2B2

    Mahsa Naghavi & Anja Geitmann

Authors
  1. Amir Sanati Nezhad
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  2. Mahmood Ghanbari
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  3. Carlos G. Agudelo
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  4. Mahsa Naghavi
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  5. Muthukumaran Packirisamy
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  6. Rama B. Bhat
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  7. Anja Geitmann
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Correspondence to Muthukumaran Packirisamy.

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Sanati Nezhad, A., Ghanbari, M., Agudelo, C.G. et al. Optimization of flow assisted entrapment of pollen grains in a microfluidic platform for tip growth analysis. Biomed Microdevices 16, 23–33 (2014). https://doi.org/10.1007/s10544-013-9802-8

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  • DOI: https://doi.org/10.1007/s10544-013-9802-8

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