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An on-chip whole blood/plasma separator with bead-packed microchannel on COC polymer

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Abstract

A disposable on-chip whole blood/plasma separator, which is able to separate plasma from whole human blood by capillary force through a bead-packed microchannel, has been designed, fabricated and characterized in this work. Various sizes of silica beads were slurry-packed through a microchannel using a bump structure which held beads in a defined region. The bead-packed microchannel induces a capillary force which allows plasma to move forward through the bead-packed column more rapidly than red blood cells (RBCs). The blood/plasma separator with bead-packed microchannel has successfully separated plasma from the whole blood without haemolysis of RBCs. The separation method developed in this work can be applied to various on-chip stationary filtrations of RBC for point-of-care clinical diagnostics.

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Acknowledgement

The authors would like to acknowledge Mr. Matthew Sperling in University of Cincinnati Department of Emergency Medicine for procuring human blood samples.

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Authors and Affiliations

  1. Microsystems and BioMEMS Laboratory, Department of Electrical and Computer Engineering, University of Cincinnati, Cincinnati, OH, 45221, USA

    Joon S. Shim, Andrew W. Browne & Chong H. Ahn

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  1. Joon S. Shim
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  2. Andrew W. Browne
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  3. Chong H. Ahn
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Correspondence to Chong H. Ahn.

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Shim, J.S., Browne, A.W. & Ahn, C.H. An on-chip whole blood/plasma separator with bead-packed microchannel on COC polymer. Biomed Microdevices 12, 949–957 (2010). https://doi.org/10.1007/s10544-010-9449-7

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  • DOI: https://doi.org/10.1007/s10544-010-9449-7

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