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A Low Molecular Weight Isoform of Hyaluronidase: Purification from Indian Cobra (Naja naja) Venom and Partial Characterization

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Abstract

A low molecular weight isoform of hyaluronidase (NNH2) has been isolated from Indian cobra (Naja naja) venom by successive chromatography on Sephadex G-75 and CM-Sephadex C-25 columns. The apparent molecular weight determined by SDS-PAGE is 52 kD, and the pI value is 9.7. NNH2 is an endoglycosidase and exhibits in vitro absolute specificity for hyaluronan; it also hydrolyzed hyaluronan in human skin sections. NNH2 is nontoxic, but it indirectly potentiates the hemorrhagic activity of hemorrhagic complex-I. Curcumin, indomethacin, and tannic acid inhibited dose dependently the degradation of hyaluronan by NNH2.

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Authors and Affiliations

  1. Department of Biochemistry, University of Mysore, Mysore, 570 006, India

    K. S. Girish & K. Kemparaju

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  1. K. S. Girish
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  2. K. Kemparaju
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Correspondence to K. Kemparaju.

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__________

Translated from Biokhimiya, Vol. 70, No. 6, 2005, pp. 855–860.

Original Russian Text Copyright © 2005 by Girish,, Kemparaju.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM04-224, October 24, 2004.

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Girish, K.S., Kemparaju, K. A Low Molecular Weight Isoform of Hyaluronidase: Purification from Indian Cobra (Naja naja) Venom and Partial Characterization. Biochemistry (Moscow) 70, 708–712 (2005). https://doi.org/10.1007/s10541-005-0172-6

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  • DOI: https://doi.org/10.1007/s10541-005-0172-6

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