biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 61:333-341, 2017 | DOI: 10.1007/s10535-016-0694-3

Changes in antioxidant enzyme activities and gene expression in two muskmelon genotypes under progressive water stress

W. A. Ansari1,2, N. Atri2, B. Singh1, S. Pandey1,*
1 ICAR-Indian Institute of Vegetable Research, Jakhini, Varanasi, India
2 Department of Botany, M.M.V., Banaras Hindu University, Varanasi, India

Responses of two muskmelon (Cucumis melo L.) genotypes (drought tolerant SC-15 and drought susceptible EC-564755) were analyzed at 0, 7, 14, and 21 d of progressive water stress. Although water deficit caused a significant decline in relative water content, the magnitude of reduction was lower in SC-15. Electrolyte leakage, hydrogen peroxide, and malonydialdehyde generation were higher in EC-564755, whereas accumulation of proline was higher in SC-15. Higher activities of antioxidant enzymes, such as catalase, superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, and glutathione reductase, and higher expression of the respective genes were recorded in SC-15 than in EC-564755. Expressions of DREB2C and DREB3 in SC-15 revealed a fluctuating pattern with down-regulation on days 7 and 21 of water stress, whereas up-regulation was observed on day 14. Concurrently, both genes in EC-564755 showed continuous down-regulation on days 7, 14, and 21 of water stress. Expressions of RD22 and dehydrin recorded on days 7, 14, and 21 were lower in SC-15. The cluster analysis showed that, these two genotypes had a clear distinction in physiological and biochemical properties and gene expressions under water stress and the genotype SC-15 had more efficient osmoprotectant mechanism than genotype EC-564755 under water deficit conditions.

Keywords: ascorbate peroxidase; catalase; Cucumis melo; DREBs; glutathione reductase; guaiacol peroxidase; RD22; RWC; superoxide dismutase
Subjects: water stress; drought tolerance; ascorbate peroxidase; catalase; glatathione reductase; guaiacol peroxidase; superoxide dismutase; DREB; relative water content; proline; malondialdehyde; electrolyte leakage; muskmelon

Received: February 19, 2016; Revised: July 18, 2016; Accepted: July 20, 2016; Published: June 1, 2017  Show citation

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Ansari, W.A., Atri, N., Singh, B., & Pandey, S. (2017). Changes in antioxidant enzyme activities and gene expression in two muskmelon genotypes under progressive water stress. Biologia plantarum, 61(2), 333-341. doi: 10.1007/s10535-016-0694-3
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