biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 60:774-782, 2016 | DOI: 10.1007/s10535-016-0650-2

Effects of salt and alkali stress on growth, accumulation of oxalic acid, and activity of oxalic acid-metabolizing enzymes in Kochia sieversiana

Y. Ma1,2, X. P. Wang3, S. F. Zhang2, D. C. Shi3, L. X. Sheng1,*
1 School of Environment, Northeast Normal University, Changchun, P.R. China
2 Department of Basic Medicine, Changchun Medical College, Changchun, P.R. China
3 School of Life Sciences, Northeast Normal University, Changchun, P.R. China

Kochia sieversiana (Pall.) C.A. Mey. is a forage plant that can grow in extremely alkalinized grasslands at pH 10 or higher. Accumulation of a large amount of oxalic acid (OxA) is a primary characteristic of K. sieversiana. In our study, seedlings of K. sieversiana were exposed to the following conditions: non-stress, salinity (200 mM, a molar ratio of NaCl and Na2SO4 1:1), and alkali stress (200 mM, a molar ratio of NaHCO3 and Na2CO3 1:1). Growth, water content, content of organic acids (including OxA), Na+, and K+, and activities of some OxA metabolism-related enzymes were determined. Results show that glycolate oxidase was the key enzyme for OxA synthesis; however, the carboxylation of phosphoenolpyruvate (PEP) by PEP carboxylase (PEPC) probably played a minor role in the OxA-synthetic pathway. The pathway of L-ascorbic acid catabolism was not the main source of OxA accumulation, and the activity of oxalate oxidase (OxO) involved in OxA decomposition was not a limiting factor for inner OxA accumulation. Taken together, accumulation of a large amount of OxA are not related to the degradation and secretion function of OxO but largely depend upon its synthetic function.

Keywords: ascorbic acid; glycolate oxidase; oxalate oxidase; phosphoenolpyruvate carboxylase
Subjects: salinity; alkaline stress; oxalic acid; ascorbic acid; glycolate oxidase; oxalate oxidase; phosphoenolpyruvate carboxylase; water content; malic acid; sodium; potassium

Received: March 27, 2015; Revised: January 4, 2016; Accepted: March 17, 2016; Published: December 1, 2016  Show citation

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Ma, Y., Wang, X.P., Zhang, S.F., Shi, D.C., & Sheng, L.X. (2016). Effects of salt and alkali stress on growth, accumulation of oxalic acid, and activity of oxalic acid-metabolizing enzymes in Kochia sieversiana. Biologia plantarum, 60(4), 774-782. doi: 10.1007/s10535-016-0650-2
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